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Method for efficiently inducing pluripotent stem cells to be differentiated to retinal pigment epitheliums (RPE)

A technology for pluripotent stem cells and retinal pigment, which is applied in the field of high-efficiency induced pluripotent stem cells to differentiate into retinal pigment epithelial cells, can solve the problems of large batch differences, low cell yields, and cumbersome methods, so as to reduce differences and improve cell yields. The effect of high rate and long differentiation time

Active Publication Date: 2017-05-03
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods still have some disadvantages, such as time-consuming, cumbersome methods, large batch-to-batch variability and low cell yield, etc.

Method used

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  • Method for efficiently inducing pluripotent stem cells to be differentiated to retinal pigment epitheliums (RPE)
  • Method for efficiently inducing pluripotent stem cells to be differentiated to retinal pigment epitheliums (RPE)
  • Method for efficiently inducing pluripotent stem cells to be differentiated to retinal pigment epitheliums (RPE)

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Embodiment

[0033] This embodiment is a method for efficiently inducing pluripotent stem cells into retinal pigment epithelial cells.

[0034] In this embodiment, the pluripotent stem cells are human embryonic stem cells or induced pluripotent stem cells, both of which can use this method to achieve the purpose of the invention.

[0035] In this embodiment, the induction of differentiation includes the following steps:

[0036] a. Inoculate the pluripotent stem cells into the feeder layer cells, add the complete medium of stem cells, place the culture and change the medium regularly until the cells reach a fusion state;

[0037] The pluripotent stem cells described in this step are the 50th generation human embryonic stem cells; the feeder layer cells are X-ray treated mouse embryonic fibroblasts; the complete medium components of the stem cells are: DMEM / F12 basal medium, serum replacement substances, non-essential amino acids, penicillin, streptomycin, glutamine, β-mercaptoethanol and ...

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Abstract

The invention relates to a method for efficiently inducing pluripotent stem cells to be differentiated to retinal pigment epitheliums (RPE), and belongs to the biology technical field. By the adoption of the method, without adding any small molecule inducible stimulators, an in-vivo eyeball development process is simulated in vitro, and the pluripotent stem cells can be spontaneously differentiated into the RPEs. Compared with methods which have already been reported, no small molecule inducible stimulators are adopted, in-vivo eyeball development is strictly simulated, and thus a large number of RPEs differentiated from the pluripotent stem cells can be obtained simply, rapidly and efficiently.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for efficiently inducing the differentiation of pluripotent stem cells into retinal pigment epithelial cells. Background technique [0002] Degenerative retinal disease is the leading cause of blindness worldwide. In this disease, the damage and dysfunction of retinal pigmented epithelium (RPE) cells lead to degeneration of photoreceptor cells (photoreceptor), eventually affecting the patient's vision until blindness. Due to the non-regeneration of RPE and photoreceptor cells, there is currently no effective treatment for these diseases in clinical practice. Cell therapy holds more promise for the treatment of retinal degenerative diseases than any other treatment. [0003] Since pluripotent stem cells have the ability to proliferate indefinitely and differentiate into various cells, they can be used as an ideal source of transplanted cells. Reports have confirmed the fea...

Claims

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Application Information

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IPC IPC(8): C12N5/079C12N5/0735C12N5/071
Inventor 徐国彤李宗义高芙蓉吕立夏
Owner TONGJI UNIV