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Dilution for live vaccine of porcine reproductive and respiratory syndrome

A technology for respiratory syndrome and live vaccines, applied in the field of veterinary biological products, can solve problems such as low uniformity of immune titers, large vaccine differences, and allergic reactions, so as to improve the overall immune level, reduce batch-to-batch variability, The effect of enhancing the immune effect

Inactive Publication Date: 2017-05-10
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] PRRS live vaccine needs to be diluted with vaccine diluent when used. At present, water for injection or normal saline is usually used, but the immune titer of the vaccine diluted in this way is not high, and the difference between different batches of vaccines is large. There are stress reactions and allergic reactions after the diseased pigs, which greatly reduce the immune effect of PRRS live vaccine

Method used

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  • Dilution for live vaccine of porcine reproductive and respiratory syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Prepare mulberry leaf polysaccharide, comprising the following steps:

[0031] (1) After crushing the mulberry leaves, pass through a 70-mesh sieve to obtain mulberry leaf powder, which is stored in a dry environment for later use;

[0032] (2) Add the mulberry leaf powder obtained in step (1) into 75°C water for extraction for 120 minutes at a material-to-liquid ratio of 1:20 g / ml, and stir once every 20 minutes;

[0033] (3) Filter the feed liquid that has been leached in step (2), and collect the filtrate; carry out decompression concentration on the obtained filtrate to obtain a concentrated solution, and the decompression concentration conditions are: temperature 50°C, rotation speed 50r / min, vacuum degree 600mmHg ;

[0034] (4) Add absolute ethanol to the concentrated solution obtained in step (3), and after alcohol precipitation in a refrigerator at 4°C for 20 minutes, centrifuge at a speed of 5000 r / min for 20 minutes to collect insoluble matter;

[0035] (...

Embodiment 2

[0051] 1. Prepare mulberry leaf polysaccharide, comprising the following steps:

[0052] (1) After the mulberry leaves are crushed and passed through a 100-mesh sieve, the mulberry leaf powder is obtained and stored in a dry environment for later use;

[0053] (2) Add the mulberry leaf powder obtained in step (1) into water at 85°C for 100 min at a material-to-liquid ratio of 1:50 g / ml, and stir once every 30 min;

[0054](3) Filter the feed liquid that has been leached in step (2), and collect the filtrate; carry out decompression concentration on the obtained filtrate to obtain a concentrated solution, and the decompression concentration conditions are: temperature 60°C, speed 50r / min, vacuum degree 700mmHg ;

[0055] (4) Add absolute ethanol to the concentrated solution obtained in step (3), and after alcohol precipitation in a refrigerator at 4°C for 24 hours, centrifuge at a speed of 6000 r / min for 15 minutes to collect insoluble matter;

[0056] (5) Use anhydrous ether...

Embodiment 3

[0072] 1. Prepare mulberry leaf polysaccharide, comprising the following steps:

[0073] (1) After crushing the mulberry leaves, pass through an 85-mesh sieve to obtain mulberry leaf powder, which is stored in a dry environment for later use;

[0074] (2) Add the mulberry leaf powder obtained in step (1) into water at 80°C for 110 minutes at a material-to-liquid ratio of 1:35 g / ml, and stir once every 25 minutes;

[0075] (3) Filter the feed liquid that has been leached in step (2), and collect the filtrate; concentrate the obtained filtrate under reduced pressure to obtain a concentrated solution, and the conditions for concentration under reduced pressure are: temperature 55°C, rotation speed 50r / min, vacuum degree 650mmHg ;

[0076] (4) Add absolute ethanol to the concentrated solution obtained in step (3), and after alcohol precipitation in a refrigerator at 4°C for 22 hours, centrifuge at a speed of 550 r / min for 18 minutes to collect insoluble matter;

[0077] (5) Use ...

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Abstract

The invention belongs to the technical field of veterinary biological products, and in particular to a dilution for a live vaccine of porcine reproductive and respiratory syndrome. Every 1000ml of phosphate buffer with the pH being 7 contains the following Chinese medicine polysaccharide components: 1-8g of folium mori polysaccharide, 0.1-5g of coriolus versicolor polysaccharide, 1-5g of rchmannia glutnosa polysaccharide and 0.1-5g of thymopentin. The dilution disclosed by the invention has the following beneficial effects: on one hand, the difference between the vaccine batches can be reduced and the overall immune level can be improved; and on the other hand, the protection period of vaccine can be prolonged, and the immune effect of the live vaccine for porcine reproductive and respiratory syndrome can be greatly improved.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, in particular to a diluent for porcine reproductive and respiratory syndrome live vaccine. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious infectious disease of pigs caused by porcine reproductive and respiratory syndrome virus (PRRSV). Pigs of different ages, breeds and sexes can be infected , the disease is a highly pathogenic disease characterized by reproductive impairment in sows, respiratory symptoms in piglets, immunosuppression, high infection rate, and high mortality. The disease has caused severe economic losses to the swine industry worldwide. [0003] In order to effectively control porcine reproductive and respiratory syndrome, a variety of vaccines for preventing PRRS have been developed in China. In the development of live PRRS vaccines, vaccine strains, virus culture conditions and immune enhancers ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/12A61K47/36A61K47/18A61P31/14
CPCA61K39/39A61K39/12A61K47/183A61K47/36A61K2039/55516A61K2039/55583C12N2770/10034
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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