Diluent for porcine parvovirus disease live vaccine

A parvovirus and live vaccine technology, applied in the direction of virus, virus/phage, virus antigen components, etc., can solve the problems of reducing the immune effect of live vaccines against porcine parvovirus disease, the uniformity of immune titers, and the large differences in vaccines. , to achieve the effect of improving the immune effect, improving the overall immune level, and enhancing the immune performance

Active Publication Date: 2020-09-22
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Porcine parvovirus disease live vaccine needs to be diluted with vaccine diluent when used. At present, water for injection or normal saline is usually used, but the immune titer of the vaccine diluted in this way is not high, and the difference between different batches of vaccines is large. , and there are stress reactions and allergic reactions after inoculation of diseased pigs, which greatly reduces the immune effect of porcine parvovirus disease live vaccine

Method used

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  • Diluent for porcine parvovirus disease live vaccine
  • Diluent for porcine parvovirus disease live vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Prepare the extract of Forsythia biflora, comprising the following steps:

[0024] Weigh honeysuckle and forsythia according to the weight of 1:1, mix, add 10 times the amount of 60% ethanol, ultrasonically extract for 15 minutes, collect the extract, repeat the extraction 3 times, mix the obtained extract, evaporate and concentrate, freeze-dry, that is Forsythia biflora extract can be obtained.

[0025] 2. The preparation of Lycium barbarum polysaccharide comprises the following steps:

[0026] (1) Dry the wolfberry in a 60°C incubator until it reaches a constant weight, and crush it for later use;

[0027] (2) Pretreatment: Weigh the crushed wolfberry in step (1), reflux with 90% ethanol for 1 hour, discard the solvent, collect the residue, and air-dry the residue for later use;

[0028] (3) Microwave extraction: Weigh the Lycium barbarum residue prepared in step (2), add distilled water according to the material-to-liquid ratio of 1:8, extract by microwave at a ...

Embodiment 2

[0038] 1. Prepare the extract of Forsythia biflora, comprising the following steps:

[0039] Weigh honeysuckle and forsythia according to the weight of 1:1, mix, add 12 times the amount of 50% ethanol, ultrasonically extract for 10 minutes, collect the extract, repeat the extraction 3 times, mix the obtained extract, evaporate and concentrate, freeze-dry, that is Forsythia biflora extract can be obtained.

[0040] 2. The preparation of Lycium barbarum polysaccharide comprises the following steps:

[0041] (1) Dry the wolfberry in a 60°C incubator until it reaches a constant weight, and crush it for later use;

[0042] (2) Pretreatment: Weigh the crushed wolfberry in step (1), reflux with 90% ethanol for 1 hour, discard the solvent, collect the residue, and air-dry the residue for later use;

[0043] (3) Microwave extraction: Weigh the Lycium barbarum residue prepared in step (2), add distilled water according to the material-to-liquid ratio of 1:15, extract by microwave at a...

Embodiment 3

[0053]1. Prepare the extract of Forsythia biflora, comprising the following steps:

[0054] Weigh honeysuckle and forsythia according to the weight of 1:1, mix, add 11 times the amount of 55% ethanol, ultrasonically extract for 13 minutes, collect the extract, repeat the extraction 3 times, mix the obtained extract, evaporate and concentrate, freeze-dry, that is Forsythia biflora extract can be obtained.

[0055] 2. The preparation of Lycium barbarum polysaccharide comprises the following steps:

[0056] (1) Dry the wolfberry in a 60°C incubator until it reaches a constant weight, and crush it for later use;

[0057] (2) Pretreatment: Weigh the crushed wolfberry in step (1), reflux with 90% ethanol for 1 hour, discard the solvent, collect the residue, and air-dry the residue for later use;

[0058] (3) Microwave extraction: Weigh the Lycium barbarum residue prepared in step (2), add distilled water according to the material-to-liquid ratio of 1:10, extract by microwave at 33...

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Abstract

The invention belongs to the technical field of veterinary biological products, and concretely relates to a diluent used for an inactive porcine parvovirus vaccine. Every 1000 ml of a phosphate buffer solution with the pH value of 7 includes 1-5 g of honeysuckle flower and Weeping Forsythia extract, 5-10 g of wolfberry fruit polysaccharides, 20-80 mg of astragaloside II and 1-5 g of polyinosinic-polycytidylic acid. The diluent used for the inactive porcine parvovirus vaccine has the advantages of convenience in production, low cost, convenience and safety in use, reduction of the difference among different batches of vaccines, and improvement of the integral immunizing effect of the vaccine.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, in particular to a diluent for porcine parvovirus disease live vaccine. Background technique [0002] Porcine parvovirus (PPV) belongs to the Parvovirus family (Paruovirdae), and is one of the main pathogens that cause reproductive disorders in pigs, especially reproductive disorders in primiparous sows. The conception rate is reduced, the number of live litters is reduced and / or premature births, miscarriages, deformed fetuses, stillbirths, mummified fetuses, weak litters, etc. occur. The virus can survive for several months in the environment and has strong resistance to most disinfectants. At present, the virus has spread all over the world, bringing huge economic losses to the pig industry and seriously hindering the development of the pig industry. healthy growth. PPV has a single serotype, and immunization is the main measure to prevent this disease. [0003] Live ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/634A61K47/02A61P37/04A61K31/715A61K31/7048A61K31/713A61K39/23
CPCA61K31/7048A61K31/713A61K31/715A61K36/355A61K36/634A61K39/12A61K47/02A61K2236/333A61K2236/39C12N2750/14334A61K2300/00
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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