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A kind of thermophilic lactobacillus and corn soaking method artificially adding the thermophilic lactobacillus

A technology of lactic acid bacteria and corn soaking, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of poor quality of corn steep liquor, low lactic acid content, short soaking time, etc., to achieve convenient control and promote softening And expansion, enhance the effect of soaking effect

Active Publication Date: 2020-04-28
COFCO BIO CHEM ENERGY LONGJIANG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the deficiencies of the prior art. In order to solve the phenomenon that the corn soaking time is short and the lactic acid content is low in the current corn soaking method, resulting in poor corn steep liquor quality, a thermophilic lactobacillus and artificially added The corn soaking method of thermophilic Lactobacillus, which is simple to operate and convenient to control, can effectively solve the poor quality of corn steep liquor in the corn soaking process, effectively improve the acid production rate of glutamic acid, and reduce production costs.

Method used

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  • A kind of thermophilic lactobacillus and corn soaking method artificially adding the thermophilic lactobacillus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] There are two kinds of substratum used in the example in this embodiment:

[0042] (1) Thermophilic lactic acid bacteria seed medium: glucose 20g / L, yeast extract 5g / L, peptone 5g / L, corn steep liquor 5g / L, molasses 2g / L, K 2 HPO 4 1.0g / L, MnSO 4 0.00025g / L, V B1 0.0001g / L, Na 2 HPO 4 1.5g / L, the solvent is water, pure biotin 5μg / L, calcium carbonate 5g / L. pH is 6.5, sterilized at 121°C for 20 minutes;

[0043] (2) Thermosensitive glutamic acid fermentation medium: starch hydrolysis sugar 40g / L; corn steep liquor 50ml / L; protein hydrolyzate 10g / L; molasses 5ml / L; disodium hydrogen phosphate 7g / L; potassium chloride 2g / L; magnesium sulfate 1.4g / L; defoamer 0.1ml / L, solvent is water, pH is 6.9-7.1, sterilized at 121°C for 20 minutes.

[0044] Supplementary sugar: Supplementary sugar solution is a glucose solution with a mass concentration of glucose of 600g / L, and is sterilized at 115°C for 20 minutes.

[0045] A corn soaking method artificially adding thermophil...

Embodiment 2

[0057] There are two kinds of substratum used in the example in this embodiment:

[0058] (1) Thermophilic lactic acid bacteria seed medium: glucose 20g / L, yeast extract 10g / L, peptone 5g / L, corn steep liquor 5g / L, molasses 2g / L, K 2 HPO 4 1.0g / L, MnSO 4 0.00025g / L, V B1 0.0001g / L, Na 2 HPO 4 1.5g / L, the solvent is water, pure biotin 5μg / L, calcium carbonate 5g / L. pH is 6.5, sterilized at 115°C for 20 minutes;

[0059] (2) Thermosensitive glutamic acid fermentation medium: starch hydrolyzed sugar 50g / L; corn steep liquor 55ml / L; protein hydrolyzate 15g / L; molasses 10ml / L; disodium hydrogen phosphate 8g / L; potassium chloride 3g / L; magnesium sulfate 1.5g / L; defoamer 0.2ml / L, solvent is water, pH is 6.9-7.1, sterilized at 121°C for 20 minutes.

[0060] Supplementary sugar: Supplementary sugar solution is a glucose solution with a mass concentration of glucose of 600g / L, and is sterilized at 115°C for 20 minutes.

[0061] A corn soaking method artificially adding thermoph...

Embodiment 3

[0077] The culture medium used in this embodiment has two kinds:

[0078] (1) Thermophilic lactic acid bacteria seed medium: glucose 30g / L, yeast extract 10g / L, peptone 5g / L, corn steep liquor 5g / L, molasses 2g / L, K 2 HPO 4 1.0g / L, MnSO 4 0.00025g / L, V B1 0.0001g / L, Na 2 HPO 4 1.5g / L, the solvent is water, pure biotin 5μg / L, calcium carbonate 5g / L. pH is 6.5, sterilized at 115°C for 20 minutes;

[0079] (2) Thermosensitive glutamic acid fermentation medium: starch hydrolysis sugar 60g / L; corn steep liquor 60ml / L; protein hydrolyzate 20g / L; molasses 15ml / L; disodium hydrogen phosphate 9g / L; potassium chloride 4g / L; magnesium sulfate 1.6g / L; defoamer 0.3ml / L, solvent is water, pH is 6.9-7.1, sterilized at 121°C for 20 minutes.

[0080] Supplementary sugar: Supplementary sugar solution is a glucose solution with a mass concentration of glucose of 600g / L, and is sterilized at 115°C for 20 minutes.

[0081] A corn steeping method artificially added with thermophilic lactob...

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Abstract

The invention relates to a thermophilic lactic acid bacterium and a corn soaking method artificially adding the thermophilic lactic acid bacterium. The thermophilic lactic acid bacterium is named C15, the classification name is Bacillus sp., and the preservation number is: CGMCC No.13471, date of deposit: December 19, 2016, No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, deposit unit: General Microbiology Center of China Committee for the Collection of Microbial Cultures. The method of the present invention enhances the soaking effect of corn grains by adding a certain amount of thermophilic lactic acid bacteria to the corn soaking liquid, shortens the corn soaking time, improves the quality of the corn soaking liquid, and ensures the temperature-sensitive glutamine with high stability The acid production level and conversion rate of acid fermentation provide good production conditions for the production of monosodium glutamate and reduce the production cost of monosodium glutamate.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering and relates to the production of corn soaking, in particular to a thermophilic lactobacillus and a corn soaking method artificially adding the thermophilic lactobacillus. Background technique [0002] The preparation of cornstarch is divided into dry method and wet method. The so-called dry method refers to the method of grinding, sieving and winnowing to separate the germ and fiber to obtain low-fat corn flour. The wet method refers to "one soaking, two grinding and three minutes", that is, soaking corn in warm water, coarse grinding, fine grinding, separation of germ, cellulose and protein, and obtaining high-purity starch. In order to obtain high-purity cornstarch, a closed wet process is generally used. [0003] Corn steep liquor is rich in amino acids and vitamins, which can be used as a cheap organic nitrogen source in the production of glutamic acid fermentatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/56C12P19/04A23L27/22A23L27/24A23L7/104C12R1/23
CPCC12P7/56C12P19/04C12N1/205C12R2001/225C12R2001/23
Inventor 佟毅丛泽峰张秀荣李越韩隽高淑平颜君刘洪号马守国周杰
Owner COFCO BIO CHEM ENERGY LONGJIANG CO LTD
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