Detoxification and rapid propagation method of sweet potatoes of Yizi 138

A sweet potato virus and sweet potato technology, which is applied in the field of sweet potato detoxification, can solve the problems of insignificant detoxification effect, influence on income, and degeneration of species, and achieve the effect of good detoxification effect and improvement of planting income.

Inactive Publication Date: 2017-05-17
TIANJIN FENGHUA YULONG AGRI DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Legacy 138 Sweet potatoes are asexually propagated by vegetative organs such as potato tubers and stems, which can easily cause the accumulation and spread of sweet potato virus diseases during the process of introduction, reproduction and production, resulting in reduced yield, reduced quality, and degraded species.
Therefore, the use of healthy seedlings for rapid propagation has become an inevitable choice, but the general detoxification method is not very effective in detoxification, which not only consumes raw materials, but also affects profits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A virus-free and rapid propagation method of Yizi 138 sweet potato, the specific steps are:

[0021] (1) Obtaining of sterile explants:

[0022] Select the top 1.5cm-long stem tip of Yizi 138 sweet potato seedlings, soak it in 70% alcohol for 10 seconds, then disinfect it with 0.1% mercuric chloride for 8 minutes, then rinse it with sterile water 8 times, and put it on the ultra-clean workbench. Above, the shoot apex was peeled off under observation with a dissecting microscope, and a 0.3mm shoot apex meristem was obtained;

[0023] (2) shoot apical meristem culture detoxification:

[0024] Inoculate the shoot apex meristem obtained by stripping on the shoot apex induction medium, and place it under the condition that the temperature is 30°C and the light intensity is 3000lx, and carry out 18h light culture every day for 18 days, and transfer it into On the common culture medium, carry out 15h light culture every day, the culture time is 60 days, obtain the test-tube ...

Embodiment 2

[0034] A virus-free and rapid propagation method of Yizi 138 sweet potato, the specific steps are:

[0035] (1) Obtaining of sterile explants:

[0036] Select the top 1.5cm-long stem tip of Yizi 138 sweet potato seedlings, soak it in 70% alcohol for 15 seconds, then disinfect it with 0.1% mercury chloride for 6 minutes, then rinse it with sterile water for 6 times, and put it on the ultra-clean workbench. Above, the shoot apex was peeled off under observation with a dissecting microscope, and a 0.4mm shoot apex meristem was obtained;

[0037] (2) shoot apical meristem culture detoxification:

[0038] Inoculate the shoot apex meristem obtained by stripping on the shoot apex induction medium, place it still under the condition that the temperature is 30°C and the light intensity is 3000lx, and carry out 18h light culture every day for 25 days, and transfer it into On the common culture medium, carry out 15h light culture every day, the culture time is 55 days, obtain the test-...

Embodiment 3

[0048] A virus-free and rapid propagation method of Yizi 138 sweet potato, the specific steps are:

[0049] (1) Obtaining of sterile explants:

[0050] Select the top 1.5cm-long stem tip of Yizi 138 sweet potato seedlings, soak it in 70% alcohol for 13s, then disinfect it with 0.1% mercuric chloride for 7 minutes, then rinse it with sterile water for 7 times, and put it on the ultra-clean workbench. Above, the shoot apex was peeled off under observation with a dissecting microscope, and a 0.3mm shoot apex meristem was obtained;

[0051] (2) shoot apical meristem culture detoxification:

[0052] Inoculate the shoot apex meristem obtained by stripping on the shoot apex induction medium, and place it under the condition that the temperature is 30°C and the light intensity is 3000lx, and carry out 18h light culture every day for 22 days, and transfer it into On the common culture medium, carry out 15h light culture every day, the culture time is 57 days, obtain the test-tube pla...

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PUM

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Abstract

The invention discloses a detoxification and rapid propagation method of sweet potatoes of Yizi 138. The detoxification and rapid propagation method comprises the following specific steps: treating stem tips at the top of the sweet potato of Yizi 138 to obtain a stem tip meristematic tissue; inoculating the stem tip meristematic tissue to a stem tip induction medium for culture to obtain a stem tip test-tube plantlet; firstly weeding out weak seedlings and symptomatic appearance seedlings through a visual observation method, then authenticating sweet potato viruses by a serological method, and weeding out non-detoxified sweet potato seedlings of Yizi 138; under a germ-free condition, cutting the high-quality sweet potato seedling stem tips subjected to stem tip meristematic tissue culture and detoxification authentication into single sections, inoculating the single sections into a common culture medium for continuous switching culture, and carrying out rapid propagation on the detoxified sweet potato seedlings of Yizi 138. According to the simple and feasible detoxification and rapid propagation method of the sweet potatoes of Yizi 138, an extremely good detoxification effect can be achieved; a large quantity of high-quality seedlings are provided; the high-quality seedlings and healthy seedlings are used for rapid propagation, so that the planting benefit can be increased, and most farmers and development enterprises are benefited.

Description

technical field [0001] The invention relates to the field of detoxification of sweet potatoes, in particular to a method for detoxification and rapid propagation of Yizi 138 sweet potatoes. Background technique [0002] Yizi 138 sweet potato has good taste, high sugar content, and good stickiness. It is one of the varieties that people in urban and rural areas like to eat. It is suitable for cultivation as spring and summer potato. It has better resistance to fertilizer and waterlogging, more pieces, and the size of potato pieces is more uniform and moderate. Yizi 138 Sweet potatoes are asexually propagated by vegetative organs such as tubers and stems. During the process of introduction, reproduction and production, it is easy to cause the accumulation and spread of sweet potato virus diseases, which will lead to problems such as yield reduction, quality decline, and species degradation. Therefore, the use of healthy seedlings for rapid propagation has become an inevitable...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王立国郭子江张嘉李建军朱永伶芮淑会王鸿鸣李彦阳
Owner TIANJIN FENGHUA YULONG AGRI DEV CO LTD
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