Microalgae cultivation method

Abstract

The invention provides a microalgae cultivation method, belonging to the technical field of microalgae cultivation, capable of avoiding the frequent addition of nitrogen sources during the microalgae cultivation process, and avoiding the reduction of microalgae output due to untimely supplementation of nitrogen sources. The method includes S1. According to the characteristics of the microalgae to be cultured, configure a nutrient solution A, the nutrient solution A does not contain a nitrogen source, adjust the pH value of the nutrient solution A to be less than or equal to 7.0, and then add ammonium nitrogen and Nitrate nitrogen is used as a nitrogen source, configured as a nutrient solution B, wherein the content of ammonium nitrogen is lower than that of nitrate nitrogen; S2, inserting the algae species of the microalgae to be cultured into the nutrient solution B, and cultivating ; S3, monitor the content of nitrate nitrogen in the culture liquid during the cultivation process, if the content of nitrate nitrogen is lower than the initial content of nitrate nitrogen, add ammonium nitrogen to the culture liquid, and add ammonium nitrogen in the culture liquid The content is not higher than the initial content of ammonium nitrogen. The method can be used for microalgae cultivation.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation, in particular to a microalgae cultivation method. Background technique [0002] In today's world where fossil resources are increasingly depleted, the search for alternative green energy has received widespread attention. Microalgae is a kind of biological resources with great application value. [0003] In the process of culturing microalgae, it is necessary to add inorganic salts, nitrogen sources and phosphorus sources to the nutrient solution. Ammonium nitrogen can be directly used to synthesize amino acids in microalgae, so ammonium nitrogen is usually added to the nutrient solution as a nitrogen source. However, microalgae are more sensitive to ammonium nitrogen. If the concentration of ammonium nitrogen is high, it will have a toxic effect on the microalgae cells, and if the concentration is low, it cannot meet the growth needs of microalgae. Therefore, in the process of ...

AI Technical Summary

Problems solved by technology

However, microalgae are more sensitive to ammonium nitrogen. If the concentration of ammonium nitrogen is high, it will have a toxic effect on microalgae cells, and if the concentration is low, it will not be able to meet the growth needs of microalgae.

Therefore, in the process of microalgae cultivation, the nitrogen source required for microalgae growth is usually provided by frequent supplementation of an appropriate concentration of ammonium nitrogen, and often the lack of nitrogen source reduces the yield of microalgae due to the lack of supplementation in time.