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Glycyrrhiza inflate bat oligosaccharide and preparation method thereof

A technology for expanding fruit licorice and oligosaccharides, which is applied in the field of biomedicine and achieves the effects of improving immune-promoting activity, strengthening spleen lymphocyte proliferation, and promoting spleen lymphocyte proliferation.

Inactive Publication Date: 2017-05-17
XINJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention provides a oligosaccharide of Glycyrrhiza bulging fruit and its preparation method, which overcomes the deficiencies of the prior art and can effectively solve the problem of using Glycyrrhiza bulging polysaccharide to prepare oligosaccharides, which has not been reported at home and abroad. The problem

Method used

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  • Glycyrrhiza inflate bat oligosaccharide and preparation method thereof
  • Glycyrrhiza inflate bat oligosaccharide and preparation method thereof
  • Glycyrrhiza inflate bat oligosaccharide and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1, the Glycyrrhizae oligosaccharides obtained by the following preparation method:

[0030] The first step, the preparation of pectic acid, take the appropriate amount of Glycyrrhizae polysaccharide, dissolve in 1.0mol / L NaOH absolute ethanol solution of 25 times to 50 times the volume of Glycyrrhizae polysaccharide, react in an ice bath for 4.5h to 5h, and react After standing overnight at a temperature of 4°C, filter under reduced pressure after standing overnight. After filtration, the precipitate is dissolved in 50% ethanol aqueous solution with a volume percentage of 50%. Adjust the pH to 1.5 to 2.0 with acid and continue the reaction for 0.5h to 1h. After filtration, the precipitate is then dissolved in 50% ethanol aqueous solution by volume, the pH is adjusted to 1.5 to 2.0 with acid, and the reaction is continued for 0.5h to 1h. After the reaction, it is filtered under reduced pressure, and the precipitation after filtration is 50% by volume % ethanol...

Embodiment 2

[0032] Embodiment 2, this Glycyrrhizae oligosaccharides of inflating fruit obtains according to the following preparation method:

[0033] The first step, the preparation of pectic acid, take an appropriate amount of Glycyrrhizae polysaccharide, dissolve it in 1.0mol / L NaOH absolute ethanol solution of 25 to 50 times the volume of Glycyrrhizae polysaccharide, react in an ice bath for 4.5h to 5h, and react After standing overnight at a temperature of 4°C, filter under reduced pressure after standing overnight, and the precipitate after filtration is dissolved in 50% ethanol aqueous solution with an acidic pH of 1.5 to 2.0 and then continue to react for 0.5h to 1h. After filtration, the precipitate is then dissolved in 50% ethanol aqueous solution by volume, and the pH is adjusted to 1.5 to 2.0 with acid, and the reaction is continued for 0.5h to 1h. After the reaction, it is filtered under reduced pressure, and the precipitation after filtration is 50% by volume. % ethanol aque...

Embodiment 3

[0035] Example 3, as an optimization of the above example, the temperature of the ice bath is 0°C to 4°C; or / and, the time for standing overnight is 12h to 24h.

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Abstract

The invention relates to the technical field of biological medicines, in particular to glycyrrhiza inflate bat oligosaccharide and a preparation method thereof. The preparation method includes the steps: first, preparing pectic acid, dissolving an appropriate amount of glycyrrhiza inflate bat polysaccharide in 1.0mol / L NaOH absolute ethyl alcohol solution with the volume accounting for 25-50 times that of the glycyrrhiza inflate bat polysaccharide, performing reaction in an ice bath for 4.5-5 hours, standing overnight at the temperature of 4 DEG C after reaction, and then performing filtration under reduced pressure. The GiP (glycyrrhiza inflate bat polysaccharide) is de-esterified to obtain the pectic acid, and enzymolysis is performed after taking the pectic acid as a substrate to obtain the glycyrrhiza inflate bat oligosaccharide. The glycyrrhiza inflate bat oligosaccharide comprises polygalactose aldehyde acid oligosaccharide with the polymerization degree of 1-5, has stronger mice spleen lymphocytes proliferation promoting functions as compared with original GiP, displays good immunological enhancement activity and has a potential application prospect in the field of food and medicines.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and relates to licorice inflating oligosaccharides and a preparation method thereof. Background technique [0002] Glycyrrhiza spp. is a traditional Chinese medicine, which has the effects of clearing heat and detoxifying, invigorating the spleen, nourishing qi, moistening the lungs and relieving cough. Glycyrrhiza polysacchiade (GP) is another important active substance besides glycyrrhizic acid and licorice flavonoids. Many physiological activities of licorice, especially the immunomodulatory activity and antitumor activity, are closely related to the polysaccharides contained in it. [0003] Glycyrrhiza inflata Bat. is a traditional Chinese medicine resource with regional characteristics in Xinjiang. Its output ranks first in the country. It is the main source of commercial licorice in Xinjiang. L.) are recorded together in various editions of the Chinese Pharmacopoeia as the base plant o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12P19/04C12P19/12C07H3/06C07H3/04C07H3/02
CPCC07H3/02C07H3/04C07H3/06C12P19/02C12P19/04C12P19/12
Inventor 丛媛媛帕丽达·阿不力孜米仁沙·亚库甫陈橙热米拉·米吉提
Owner XINJIANG MEDICAL UNIV
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