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Serum type 4 fowl adenovirus inactivated vaccine and preparation method thereof

A technology of poultry adenovirus and inactivated vaccines, applied in biochemical equipment and methods, antiviral agents, viruses/bacteriophages, etc., can solve the problem of low titer, difficulty in meeting the requirements of large-scale vaccine production, and failure to meet the high titer of vaccine production and other issues, to achieve the effects of reducing costs, providing vaccine immunity, and good application prospects

Inactive Publication Date: 2017-05-24
YANGZHOU UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although serum type 4 FAdV virus can grow and replicate in chicken embryos, the titer of serum type 4 FAdV virus amplified in chicken embryos is low, which cannot meet the requirements of high titer for vaccine production
In addition, although the serotype 4 FAdV virus can effectively replicate in chicken hepatocytes, it is difficult for ordinary cell culture to meet the large-scale production requirements of vaccines.

Method used

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  • Serum type 4 fowl adenovirus inactivated vaccine and preparation method thereof
  • Serum type 4 fowl adenovirus inactivated vaccine and preparation method thereof

Examples

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Embodiment

[0016] 1. Isolation and identification of serotype 4 avian adenovirus JH strain: take the liver of a dead chicken suspected of avian adenovirus infection, grind it and add PBS at a ratio of 1:5 to make a suspension; centrifuge at 5000r / min for 15min, take Supernatant: Add penicillin and streptomycin at 1000IU / ml each, react at 37°C for 30 minutes; filter through a 0.45um microporous filter, inoculate 8-day-old SPF chicken embryos through the allantoic cavity at a dose of 0.2ml / embryo, and inoculate Collect allantoic fluid after 96-120 hours; store at -20°C. Take 400 μL of allantoic fluid into a 1.5 mL finger tube, add 400 μL of cell lysate (50 mmol / L Tris-HCl pH 8.0, 20 mmol / L EDTA pH 8.0, 2% SDS and proteinase K), mix well and place in a 56°C water bath Pot for 4 hours; add 400uL Tris to balance phenol, mix thoroughly and centrifuge at 10000r / min for 10 minutes, take the supernatant in another 1.5mL finger tube; add 400μL phenol: chloroform: isoamyl alcohol, mix thoroughly an...

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Abstract

The invention belongs to the technical field of vaccine preparation, and in particular, relates to a serum type 4 fowl adenovirus inactivated vaccine and a preparation method thereof. The serum type 4 fowl adenovirus inactivated vaccine has an antigen which is an inactivated serum type 4 fowl adenovirus having the titer not less than 109 TCID50 / ml and obtained by a suspension culture method. With use of chicken liver cells, the serum type 4 fowl adenovirus is efficiently amplified by a riptide-type bioreactor suspension culture technology, and the high-titer serum type 4 fowl adenovirus inactivated vaccine is developed. The titer of the vaccine is 109 TCID50 / ml and is 100 times that of a traditional chick embryo vaccine. The preparation of the high-titer serum type 4 fowl adenovirus inactivated vaccine can greatly reduce the cost of the vaccine, provides the immune effect of the vaccine, and has a good prospect of application.

Description

technical field [0001] The invention belongs to the technical field of vaccine preparation, and in particular relates to an inactivated vaccine of serotype 4 avian adenovirus based on suspension culture technology. Background technique [0002] Fowl Adenovirus (Fowl Adenovirus, FAdV) belongs to Adenoviridae, and is divided into 5 species (A-E) and 12 serotypes. Although it has been reported all over the world, FAdV infection generally causes subclinical conditions, while acute infection mainly causes inclusion body hepatitis, pericardial effusion, and gizzard erosion. Since 2013, cases of inclusion body hepatitis and pericardial effusion caused by FAdV in domestic chicken flocks have gradually increased. By 2015, FAdV infection broke out in chicken flocks in several provinces in China. At present, FAdV outbreaks not only occur in 3-4 week-old broiler chickens, but also occur in 10-20 week-old laying hens, causing serious economic losses to the domestic chicken industry. V...

Claims

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Application Information

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IPC IPC(8): A61K39/235A61P31/14C12N7/00
Inventor 叶建强张建军李文迹张伟秦爱建殷健高巍邵红霞梁广成
Owner YANGZHOU UNIV
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