Nasopharyngeal carcinoma EB virus serum antibody tag detection chip and kit thereof

A detection kit and technology for Epstein-Barr virus, applied in the field of nasopharyngeal carcinoma Epstein-Barr virus serum antibody label detection chip and its kit, can solve the problems of non-commercialization, expensive equipment, and increased cost, and achieve good specificity and high efficiency. The effect of sensitivity

Inactive Publication Date: 2017-05-24
王辉云
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Problems solved by technology

The main reasons leading to this situation are as follows: First, EB virus is commonly infected in the population, and normal people also produce EB virus antibodies, and the occurrence of some tumors is also related to EB virus. Therefore, the currently used detection indicators are not very accurate. Ideally, better antigen-antibody markers need to be found; second, the specificity and sensitivity of a single serum immunological marker are limited, and multiple serum immune markers need to be detected jointly, but detecting markers with existing technologies will lead to The cost increases and t

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  • Nasopharyngeal carcinoma EB virus serum antibody tag detection chip and kit thereof
  • Nasopharyngeal carcinoma EB virus serum antibody tag detection chip and kit thereof
  • Nasopharyngeal carcinoma EB virus serum antibody tag detection chip and kit thereof

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Embodiment Construction

[0021] The technical solution of the present invention will be further described below in combination with experiments.

[0022] A high-throughput PCR recombination cloning and expression platform was used to amplify 95 ORF fragments of 84 genes in the Epstein-Barr virus genome, and clone them into the constructed recombinant plasmid vector pGEM-XTE by in vivo homologous recombination cloning method. Use an in vitro cell-free transcription / translation kit to express the protein of interest. The unpurified expressed protein was directly spotted on the glass slide covered with nitrocellulose membrane, and prepared into an Epstein-Barr virus whole proteome chip, and the EB virus serological antibody spectrum detection was performed on the collected untreated nasopharyngeal carcinoma patients and healthy people. Proteome-level screening distinguishes serum antibody profiles between nasopharyngeal carcinoma and normal subjects.

[0023] Recombinant plasmid vector construction

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Abstract

The invention discloses a nasopharyngeal carcinoma EB virus serum antibody tag detection chip and a kit thereof. Nasopharyngeal carcinoma can be detected effectively by detecting the expression of EBV serum antibody BMRF1-IgG, LF1-IgA, BNLF2b-IgA, BKRF4-IgG, BMRF1-IgA, BXLF1-IgG, BaRF1-IgG, BKRF3-IgG, BZLF1-E1-IgG and BNLF2b-IgG. The nasopharyngeal carcinoma detection chip and kit provided by the invention have very good specificity and high sensitivity, and can be effectively used for nasopharynx screening, early clinical diagnosis and prognosis evaluation, etc.

Description

technical field [0001] The invention relates to a tumor detection chip and a kit, in particular to a nasopharyngeal carcinoma Epstein-Barr virus serum antibody label detection chip and a kit. Background technique [0002] Epstein-Barr virus belongs to human herpes virus type 4 (HHV-4), which is a double-stranded DNA with a full length of 172kb and encodes 85 genes. Serological epidemiological surveys show that more than 90% of adults worldwide have been infected with this virus. In most populations, Epstein-Barr virus can remain a lifelong infection without serious consequences. Most children infected with Epstein-Barr virus have no obvious symptoms, or only cause upper respiratory tract infection. Infectious mononucleosis can occur if a primary infection occurs during adolescence. A large number of studies have shown that Epstein-Barr virus infection is related to the occurrence of many tumors, such as Burkitt's lymphoma, Hodgkin's disease, gastric cancer, nasopharyngeal...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574G01N33/569
CPCG01N33/6854G01N33/56994G01N33/57484G01N2469/20
Inventor 王辉云
Owner 王辉云
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