Nasopharyngeal carcinoma EB virus serum antibody tag detection chip and kit thereof

Abstract

The invention discloses a nasopharyngeal carcinoma EB virus serum antibody tag detection chip and a kit thereof. Nasopharyngeal carcinoma can be detected effectively by detecting the expression of EBV serum antibody BMRF1-IgG, LF1-IgA, BNLF2b-IgA, BKRF4-IgG, BMRF1-IgA, BXLF1-IgG, BaRF1-IgG, BKRF3-IgG, BZLF1-E1-IgG and BNLF2b-IgG. The nasopharyngeal carcinoma detection chip and kit provided by the invention have very good specificity and high sensitivity, and can be effectively used for nasopharynx screening, early clinical diagnosis and prognosis evaluation, etc.

Description

technical field [0001] The invention relates to a tumor detection chip and a kit, in particular to a nasopharyngeal carcinoma Epstein-Barr virus serum antibody label detection chip and a kit. Background technique [0002] Epstein-Barr virus belongs to human herpes virus type 4 (HHV-4), which is a double-stranded DNA with a full length of 172kb and encodes 85 genes. Serological epidemiological surveys show that more than 90% of adults worldwide have been infected with this virus. In most populations, Epstein-Barr virus can remain a lifelong infection without serious consequences. Most children infected with Epstein-Barr virus have no obvious symptoms, or only cause upper respiratory tract infection. Infectious mononucleosis can occur if a primary infection occurs during adolescence. A large number of studies have shown that Epstein-Barr virus infection is related to the occurrence of many tumors, such as Burkitt's lymphoma, Hodgkin's disease, gastric cancer, nasopharyngeal...

AI Technical Summary

Problems solved by technology

The main reasons leading to this situation are as follows: First, EB virus is commonly infected in the population, and normal people also produce EB virus antibodies, and the occurrence of some tumors is also related to EB virus. Therefore, the currently used detection indicators are not very accurate. Ideally, better antigen-antibody markers need to be found; second, the specificity and sensitivity of a single serum immunological marker are limited, and multiple serum immune markers need to be detected jointly, but detecting markers with existing technologies will lead to The cost increases and the efficiency decreases. If a liquid chip is used for detection, the disadvantage is that the instrument is very expensive and has not yet been commercialized; third, although many Epstein-Barr virus antigens have been studied, they are still less than 20% of all Epstein-Barr virus proteins. %, and these antigens are mainly limited to capsid antigens, early antigens, membrane antigens, nuclear antigens, nucleic acid polymerases, and activating factors. Insufficient, and failed to systematically and comprehensively observe the serum immune response caused by all Epstein-Barr virus proteins in humans

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