Artificial maturation method of cephalopods
A cephalopod and artificial technology, applied in the direction of pharmaceutical formula, medical preparations containing active ingredients, animal husbandry, etc., can solve the problems of complex production process and limited release period, and achieve simple operation steps, uniform release speed, and release long time effect
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Embodiment 1
[0015] A method for artificially promoting maturation of cephalopods, the specific steps are: soaking cephalopods in seawater containing 2.5-3.8% polyurethane for anesthesia, preventing inkjet when implanting GnRH slow-release embedding blocks, soaking time is 60 ~100s. A hole was drilled at the orbital sinus of the cephalopod, and the GnRH slow-release embedding block was pushed in with forceps and allowed to float freely in the sinus. Wherein, the preparation steps of the GnRH sustained-release embedding block are:
[0016] 1. Preparation of PLGA powder: Add LA and LG in a molar ratio of 70:30~90:10 into the reaction kettle, vacuumize, and distill under reduced pressure at 70~80°C for 0.8~1.2h to remove 10~20% of the water in the raw materials , add the catalyst stannous chloride, p-toluenesulfonic acid and arachidonic acid, the addition amount is 0.2~0.5%, 0.4~0.8%, 0.001~0.003% of the raw material quality, heating, the temperature is controlled at 120~130 ℃, react in low...
Embodiment 2
[0021] A method for artificially promoting maturation of cephalopods. Taking sea octopus as an example, the sea octopus is anesthetized, and optic glands or GnRH slow-release embedding blocks are implanted in the orbital sinuses under the eyes to achieve the effect of promoting maturation and The purpose of inducing labor. Since the sea octopus is a calcareous endoskeleton, it should not be damaged after implantation; at the same time, because it is loose and porous, it is also easy for hormones to be released into the body to play a role.
[0022] The specific steps are as follows: immerse the squid octopus in seawater containing 3% polyurethane for anesthesia, and prevent inkjet when the GnRH slow-release embedding block is implanted, and the soaking time is 70s. A hole was drilled at the orbital sinus of the sea slug, and the GnRH slow-release embedding block was pushed in with tweezers and allowed to float freely in the sinus. Wherein, the preparation steps of the GnRH su...
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