Preparation method and application of microcapsules based on gelation of tobacco protein and tobacco polysaccharide
A technology of polysaccharide gel and microcapsules, which is applied in the field of tobacco manufacturing, can solve the problems of loss of heat-sensitive substances and destruction of aroma substances, and achieve the effect of solving serious losses and improving sensory quality
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[0020] A preparation method and application of microcapsules based on gelatinization of tobacco protein and tobacco polysaccharide, comprising the following steps:
[0021] Step 1: Preparation of tobacco protein-enriched phase: put tobacco leaf raw materials into an extraction tank whose extraction solvent is water, add protein co-solvent, control a certain temperature, stir and extract for a period of time, and perform solid-liquid separation to obtain crude tobacco protein Extraction. Tobacco protein is adsorbed by macroporous adsorption resin, eluted by ethanol aqueous solution, and concentrated into a certain concentration of tobacco protein rich phase.
[0022] Step 2: Preparation of Tobacco Polysaccharide-enriched Phase: Put the tobacco stem raw material into an extraction tank with water as the extraction solvent, control a certain temperature, stir and extract for a period of time, and perform solid-liquid separation to obtain crude tobacco polysaccharide extract. A c...
Embodiment 1
[0026] Step 1: Preparation of tobacco protein-enriched phase: put Burley tobacco fragments into an extraction tank whose extraction solvent is water, add 3 parts of urea, 8 parts of sodium chloride, 38 parts of ammonium sulfate, and 51 parts of sodium sulfate. The numbers are parts by weight. Stirring at a temperature of 55° C. and a stirring speed of 100 r / min for 1.5 h to obtain crude tobacco protein extract. Tobacco protein is adsorbed by D3520 macroporous adsorption resin, eluted with 85% ethanol aqueous solution, and concentrated into a certain concentration of tobacco protein enriched phase.
[0027] Step 2: Preparation of the tobacco polysaccharide-rich phase: put the tobacco stem raw material into an extraction tank whose extraction solvent is water, stir at a temperature of 60°C and a stirring speed of 100r / min for 1.5h, and perform solid-liquid separation to obtain tobacco Crude polysaccharide extract. Adding 90% ethanol aqueous solution to the crude tobacco polysa...
Embodiment 2
[0031] Step 1: Preparation of tobacco protein-enriched phase: Burley tobacco fragments are put into an extraction tank in which the extraction solvent is water, and the parts by weight are added to form: 5 parts of urea, 7 parts of sodium chloride, 45 parts of ammonium sulfate, 43 parts of Parts Sodium Sulfate. Stirring at a temperature of 50° C. and a stirring speed of 100 r / min for 1.5 h to obtain crude tobacco protein extract. Tobacco protein is adsorbed by D3520 macroporous adsorption resin, eluted with 85% ethanol aqueous solution, and concentrated into a certain concentration of tobacco protein enriched phase.
[0032] Step 2: Preparation of the tobacco polysaccharide-rich phase: put the tobacco stem raw material into an extraction tank whose extraction solvent is water, stir at a temperature of 65°C and a stirring speed of 100r / min for 1.5h, and perform solid-liquid separation to obtain tobacco Crude polysaccharide extract. Adding 95% ethanol aqueous solution to the t...
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