Bovine viral diarrhea, bovine infectious rhinotracheitis, bovine parainfluenza triple inactivated vaccine and preparation method thereof

A technology for bovine viral diarrhea and inactivated vaccine, applied in the field of veterinary biological products, can solve the problems of slow growth of beef cattle and recovered cattle, decline of milk production, loss of cattle industry, etc.

Active Publication Date: 2019-12-06
QILU ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Viral infection can induce reduced milk production and abortion, and lead to slow growth of beef cattle and rehabilitation cattle, causing great losses to the cattle industry

Method used

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  • Bovine viral diarrhea, bovine infectious rhinotracheitis, bovine parainfluenza triple inactivated vaccine and preparation method thereof
  • Bovine viral diarrhea, bovine infectious rhinotracheitis, bovine parainfluenza triple inactivated vaccine and preparation method thereof
  • Bovine viral diarrhea, bovine infectious rhinotracheitis, bovine parainfluenza triple inactivated vaccine and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0065] ——Preparation of bovine viral diarrhea, bovine infectious rhinotracheitis, bovine parainfluenza triple inactivated vaccine (L strain + J1 strain + S strain)

[0066] 1. Antigen Preparation

[0067] In order to solve a series of problems that BVDV, IBRV, BPIV3 adapt to spinner bottle adherent culture, suspension culture, microcarrier suspension culture cell bovine kidney cell line MDBK (from China Veterinary Microorganism Culture Preservation Management Center) process, the invention provides The invention discloses a method for preparing BVDV-L strain, IBRV-J1 strain and BPIV3-S strain by utilizing spinner bottle adherent culture, suspension culture technology and microcarrier suspension culture technology.

[0068] (1) Prepare BVDV-L strain, IBRV-J1 strain, and BPIV3-S strain antigens by using spinner bottle adherent culture cells

[0069] 1) Cultivate cells: First, resuscitate the bovine kidney cell line MDBK into a cell flask, grow for about 48 hours, digest with ED...

Embodiment 2

[0091] ——The finished product inspection of bovine viral diarrhea, bovine infectious rhinotracheitis, and bovine parainfluenza triple inactivated vaccine (L strain + J1 strain + S strain)

[0092] 1. Sterility test: according to the appendix of the current "Chinese Veterinary Pharmacopoeia", it should meet the requirements. The above test results are shown in Table 2.

[0093] Table 2 Physical properties, sterility test results

[0094]

[0095] 2. Safety inspection:

[0096] (1) Substitute animal test: use 6 healthy rabbits weighing 1.5 to 2.0 kg, including 4 in the immunization group and 2 in the control group, inject 1.0ml of vaccine into each leg muscle of the rabbits in the immunization group (two-point injection, 0.5ml / point), each leg of rabbits in the control group was injected intramuscularly with 1.0ml of cell culture (two-point injection, 0.5ml / point), observed continuously for 7 days and measured body temperature at fixed points every afternoon. There is no ...

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Abstract

The invention relates to a triple inactivated vaccine for preventing bovine viral diarrhea, infectious bovine rhinotracheitis and bovine parainfluenza and preparation method thereof. The active ingredients of the vaccine include the inactivated antigens of bovine viral diarrhea virus, infectious bovine rhinotracheitis virus and bovine parainfluenza type-3 virus; and in the triple inactivated vaccine, virus multiplication is performed by use of a passage cell line of cell spinner bottle, full suspension and micro-carrier culture, and the vaccine preparation technology and the like are optimized. The safety and efficacy test results of the vaccine indicate that after bovine is immunized with the triple inactivated vaccine provided by the invention, local and whole-body adverse reaction is avoided, and all bovines gain immune protection, thus the vaccine is safe and reliable, an aim of 'one injection and three preventions' is achieved, and economic loss is reduced.

Description

technical field [0001] The invention relates to a bovine viral diarrhea, bovine infectious rhinotracheitis, and bovine parainfluenza triple inactivated vaccine and a preparation method thereof, belonging to the field of veterinary biological products. Background technique [0002] Bovine viral diarrhea (BVD) is a viral infectious disease caused by Bovine viral diarrhea virus (BVDV) belonging to Flaviviridae and Pestivirus genus, mainly affecting cattle, sheep, Deer, cattle, pigs and other animals, cattle of all ages are susceptible. The main clinical manifestations are diarrhea, acute and chronic mucosal diseases, persistent infection and immune tolerance, immunosuppression, abortion or deformed fetuses in pregnant dams. The disease was first discovered in the United States by Olafson et al. in 1946. It is characterized by fever, diarrhea and cough, and is called bovine viral diarrhea. Ramsey and Chiverst first discovered Mucosal disease (MD) in 1953, and the American Vete...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/215A61K39/255A61K39/155A61P31/14A61P31/22
CPCA61K39/12A61K2039/70C12N2760/18634C12N2770/16034C12N2770/20034
Inventor 王蕾徐龙涛毕玉彧禚宝山高凤鞠洪涛
Owner QILU ANIMAL HEALTH PROD
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