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Extraction method of yolk antibody of aviadenovirus IV and application thereof

A technology of egg yolk antibody and extraction method, applied in the direction of antibodies, antiviral agents, antibody medical components, etc., can solve the problems of low activity, low purity of egg yolk antibody, and complicated preparation, and achieves expanded yield, important value, and low cost. Effect

Inactive Publication Date: 2017-05-31
QINGDAO YIYIHE AGRI & ANIMAL HUSBANDRY SCI & TECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Therefore, the technical problem to be solved by the present invention is to overcome the defects of low purity, cumbersome preparation and low activity in the production of egg yolk antibody in the prior art, so as to provide an avian gland with high antibody purity, no influence on activity and high yield Extraction method of egg yolk antibody of virus type Ⅳ

Method used

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Examples

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Effect test

Embodiment 1

[0035] This embodiment provides a method for extracting egg yolk antibody of avian adenovirus type IV, comprising the following steps:

[0036] S1. Select 3 healthy 17-18 week-old Hailan brown chickens, inject the immunogen (avian adenovirus type IV oil-emulsion inactivated vaccine) into the Hailan brown chickens, and the injection volume for each chicken is 0.5ml. The injection site is 4-point injection, one point on the chest, one point on each side of the wing, and one point on the muscle, a total of four points. The booster immunization is carried out on the 7th and 14th days after the first immunization. The dose and method of immunization are the same as the first immunization;

[0037] S2. Collect the eggs after the immunization, count from the last immunization, number the collected eggs and make a record, and store them in the refrigerator at 4°C for later use. Collect unimmunized eggs from Hailan brown chickens as negative controls;

[0038] S3. The specific steps of...

Embodiment 2

[0043] This embodiment provides a method for extracting egg yolk antibody of avian adenovirus type IV, comprising the following steps:

[0044] S1. Select 3 healthy 17-18 week-old Hailan brown chickens, inject the immunogen (avian adenovirus type IV oil-emulsion inactivated vaccine) into the Hailan brown chickens, and the injection volume for each chicken is 0.5ml. The injection site is 4-point injection, one point on the chest, one point on each side of the wing, and one point on the muscle, a total of four points. The booster immunization is carried out on the 7th and 14th days after the first immunization. The dose and method of immunization are the same as the first immunization;

[0045] S2. Collect the eggs after the immunization, count from the last immunization, number the collected eggs and make a record, and store them in the refrigerator at 4°C for later use. Collect unimmunized eggs from Hailan brown chickens as negative controls;

[0046] S3. The specific steps of...

Embodiment 3

[0052] This embodiment provides a method for extracting egg yolk antibody of avian adenovirus type IV, comprising the following steps:

[0053] S1. Select 3 healthy 17-18 week-old Hailan brown chickens, inject the immunogen (avian adenovirus type IV oil-emulsion inactivated vaccine) into the Hailan brown chickens, and the injection volume for each chicken is 0.5ml. The injection site is 4-point injection, one point on the chest, one point on each side of the wing, and one point on the muscle, a total of four points. The booster immunization is carried out on the 7th and 14th days after the first immunization. The dose and method of immunization are the same as the first immunization;

[0054] S2. Collect the eggs after the immunization, count from the last immunization, number the collected eggs and make a record, and store them in the refrigerator at 4°C for later use. Collect unimmunized eggs from Hailan brown chickens as negative controls;

[0055] S3. The specific steps fo...

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Abstract

The invention relates to the field of the preparation of yolk antibodies from avian eggs, and discloses an extraction method of a yolk antibody of aviadenovirus IV. The extraction method comprises the following steps of (1) using an oil-emulsion inactivated vaccine of the aviadenovirus IV as an antigen to immunize a health hen, and collecting an egg laid by the immunized hen; (2) separating yolk in the egg laid by the immunized hen to obtain water-soluble component liquor; (3) adding the water-soluble component liquor obtained by separating the yolk into an aqueous two-phase system, so as to obtain water-soluble component mixed liquor, wherein the aqueous two-phase system is prepared from 25wt% to 28wt% of polyethylene glycol and 20wt% to 25wt% of sodium citrate; (4) carrying out separation and purification on the water-soluble component mixed liquor. The invention provides the extraction method of the yolk antibody of the aviadenovirus IV, which is high in antibody purity and high in yield and does not influence activity.

Description

technical field [0001] The invention relates to the field of preparing yolk antibodies from poultry eggs, in particular to a method for extracting avian adenovirus type IV yolk antibodies and its application. Background technique [0002] Group I avian adenoviruses make up the genus Avian Adenovirus in the family Adenoviridae. Group II (Turkey Hemorrhagic Enteritis and Related Viruses) and Group III (Egg Drop Syndrome) avian adenoviruses are clearly associated with disease, in contrast to most group I adenoviruses that are less pathogenic in poultry. Not entirely sure. But FAdV-1 and FAdV-4 are obviously exceptions, among which FAdV-1 can cause quail bronchitis, and FAdV-4 is the main cause of hydropericardium hepatitis syndrome. Other avian adenovirus strains allow rapid infection of opportunistic pathogens when chicken health is compromised, such as co-infection with other pathogens such as Chicken Infectious Anemia Virus (CIAV) and Infectious Bursal Disease Virus (IBDV)...

Claims

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Application Information

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IPC IPC(8): C07K16/08C07K16/02A61K39/42A61P31/14A61P31/20
CPCA61K39/00C07K16/02C07K16/081
Inventor 杨柳枝丛桂海于海龙
Owner QINGDAO YIYIHE AGRI & ANIMAL HUSBANDRY SCI & TECH LTD
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