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Foot-and-mouth disease virus serotype-shared epitope recognized by monoclonal antibody 3d9 and its application

A technology of monoclonal antibody and foot-and-mouth disease virus, applied in antiviral agents, antiviral immunoglobulins, positive-sense single-stranded RNA viruses, etc., can solve the problems of reduced meat consumption, reduced animal production performance, and reduced meat products

Active Publication Date: 2020-12-22
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease causes the death of young animals, decreased milk production, reduced meat consumption, reduced meat production, and reduced animal production performance. It is also highly contagious and can form a large-scale epidemic, causing huge economic losses.

Method used

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  • Foot-and-mouth disease virus serotype-shared epitope recognized by monoclonal antibody 3d9 and its application
  • Foot-and-mouth disease virus serotype-shared epitope recognized by monoclonal antibody 3d9 and its application
  • Foot-and-mouth disease virus serotype-shared epitope recognized by monoclonal antibody 3d9 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The identification of the foot-and-mouth disease virus serum shared type epitope that embodiment 1 monoclonal antibody 3D9 recognizes

[0026] 1. Materials and methods

[0027] 1.1 Viruses, cells and strains

[0028] The foot-and-mouth disease virus O / YS / CHA / 05 strain belongs to the O-type pan-sublineage; the suckling hamster kidney cell BHK-21 is preserved in the inventor's laboratory; the infectious plasmid PYS of the FMDV O / YS / CHA / 05 strain is all tested by the inventor Room preservation.

[0029] The hybridoma cell line secreting foot-and-mouth disease virus shared monoclonal antibody 3D9, and its microorganism preservation number is: CGMCC No.13293.

[0030] 1.2 Main reagents

[0031] PrimeSTAR DNA polymerase was purchased from TaKaRa Company; Ph.D.-12 TM Peptide library kit, DpnI and T4 DNA ligase were purchased from New England Biolabs; HRP or FITC-labeled goat anti-mouse IgG was purchased from Sigma; HRP-labeled anti-M13 phage antibody was purchased from GE ...

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Abstract

The invention discloses a monoclonal antibody 3D9-identified foot and mouth disease virus serotype sharable epitope and application thereof. Firstly, the invention discloses the monoclonal antibody 3D9-identified foot and mouth disease virus serotype sharable epitope, an amino acid sequence is SEQ ID No: 2. The foot and mouth disease virus serotype sharable epitope is a sharable epitope comprising seven serotype foot and mouth disease viruses. In the invention, phage clone obtained from screening is subjected to specific binding with a monoclonal antibody 3D9 to be used as an indirect ELISA method for simulating antigen establishing, and positive foot and mouth disease virus serum can be detected in a specific manner. The monoclonal antibody 3D9-identified foot and mouth disease virus serotype sharable epitope disclosed by the invention has application valves in diagnosis or prevention and treatment of foot-and-mouth diseases.

Description

technical field [0001] The invention relates to the foot-and-mouth disease virus serotype shared epitope recognized by the foot-and-mouth disease virus shared type monoclonal antibody 3D9, and the invention also relates to the application of the foot-and-mouth disease virus serotype-shared epitope in the diagnosis or prevention of foot-and-mouth disease, which belongs to the field of prevention and treatment of foot-and-mouth disease . Background technique [0002] Foot-and-mouth disease (FMD), caused by foot-and-mouth disease virus (FMDV), is one of the most important infectious diseases that seriously harm even-hoofed animals. The disease causes the death of young animals, decreased milk production, decreased meat consumption, decreased meat production, and decreased animal production performance. It is highly contagious and can form a large-scale epidemic, causing huge economic losses. The key to preventing and controlling FMD is immunization and the application of accur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/10C07K14/09G01N33/577G01N33/569A61K39/42A61K39/135A61P31/14
CPCA61K39/00C07K14/005C07K16/1009C12N2770/32122G01N33/56983G01N33/577G01N2333/09
Inventor 于力周国辉王海伟杨德成
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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