The present invention relates to the field of feeds and particularly relates to a feed for laying hens and a preparation method thereof. The feed for the laying hens consists of the following raw materials in weight percentages: corn, peeled soybean meal, cottonseed meal, wheat bran, stone meal, a premix and edible salt; total phosphorus content in the corn, peeled soybean meal, cottonseed meal and wheat bran is larger than 0.35%; wherein each gram of the premix contains 1-alpha-OH-VD3, phytase, VE, VK, VB1, VB2, D-pantothenic acid, nicotinic acid, VB6, VB12, biotin, folic acid, Cu, Fe, Mn, Zn, Se, and the balance premix conventional accessory materials. The high-dose phytase and 1-alpha-OH-VD3 in the prepared feed are synergistically used to enhance digestion and absorption of calcium andphosphorus by the laying hens, can realize complete no addition of calcium hydrogen phosphate, and enables the laying hens to maintain normal production performance, not to suffer from soft leg diseases, and to have no significant differences between death and culling rate and egg production rate.