Three-dimensional perfusion culture system and tissue organ formed through 3D printing

Abstract

The invention relates to the field of tissue engineering, and in particular relates to a three-dimensional perfusion culture system and a tissue organ formed through 3D printing. The three-dimensional perfusion culture system comprises the following parts which are connected in series: a culture liquid supply part, a combined power source, a three-dimensional culture device and an oxygen / carbon dioxide circular supply part, wherein the culture liquid supply part is used or storing a culture solution for the three-dimensional perfusion culture system; the combined power source can simulate a heart supply system in the human body; the three-dimensional culture device enables the tissue organ to be cultured in a multi-freedom-degree movement manner; the oxygen / carbon dioxide circular supply part is used for simulating the lung respiratory system in the human body. With the three-dimensional perfusion culture system provided by the invention, a heart and lung simulation environment can be provided for the three-dimensional tissue organ formed through biological 3D printing and containing the tissue cells, and thus a microenvironment similar to the inside of the body is provided for the growth of the tissue organ. The invention further provides the tissue organ formed through 3D printing and cultured by the three-dimensional perfusion culture system.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a cardiopulmonary simulation three-dimensional perfusion culture system for culturing biological 3D printed tissues and 3D printed tissues and organs cultured by the three-dimensional perfusion culture system. Background technique [0002] The rise of bio-3D printing technology has had a huge impact on the development of tissue engineering in the medical field. Through 3D bioprinting technology, biocompatible biomaterials can be printed into tissue engineering (scaffolds) for tissue and / or organ repair, maintenance or improvement of damaged tissues or organs. In addition, hydrogels containing normal tissue cells (living cells) can also be printed on biomaterial scaffolds to form corresponding tissues and organs with specific shapes, structures and functions. The obtained tissues and organs containing living cells are cultured for a period of time, while the cells grow and proli...

AI Technical Summary

Problems solved by technology

However, the human body is a complex and active individual that needs a blood circulation system that continuously supplies nutrients and excretes waste. The existing perfusion culture systems cannot meet the requirements.

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