Disposable intelligent digester system for animal cell culture

Abstract

The invention discloses a disposable intelligent digester system for animal cell culture. The disposable intelligent digester system comprises a disposable digester unit and an intelligent control unit, wherein the disposable digester unit at least comprises a sealed chamber that can be filled with fluid, an interception component and a stirring component are arranged in the sealed chamber, the interception component is in surrounding connection with a lower part in the sealed chamber, the interception component is at least used for causing dispersed cells to flow to a next level bioreactor and intercepting empty carriers and carriers with cells in the sealed chamber, and the stirring component is at least used for mixing the fluid in the sealed chamber. The disposable intelligent digester system disclosed by the invention omits online sterilization, online cleaning, equipment installation and debugging and other steps, simplifies the operation flows, reduces the production cost and the labor cost, and avoids the cross contamination between batches; and moreover, the digestion process is controlled by an intelligent program, so that the digestion loss rate of the cells can be as low as 2%.

Description

technical field [0001] The invention relates to a technology for separating cells from microcarriers in the process of animal cell culture to obtain free cells, more particularly to a disposable intelligent digester system for animal cell culture. Background technique [0002] In the production of high value-added biological products such as vaccines, antibodies, and protein drugs, some mammalian cells used cannot be directly suspended in the culture medium for growth, and need to attach to the surface of a carrier with a small amount of positive charge to grow. The basic process is : Mammalian cells are mechanically dispersed or digested to make a cell suspension, filtered, centrifuged, purified, rinsed, and then inoculated into a reactor containing fresh medium for culture. The cells cultured by this method grow well and are easy to observe, but this method is only suitable for laboratory research. However, in the process of large-scale cell culture, inoculation and expan...

AI Technical Summary

Problems solved by technology

After each use, in order to ensure the aseptic cleaning effect of the digester, it must be disassembled, cleaned and sterilized repeatedly. The cleaning process itself is a time-consuming and laborious process, and the cleaning method used increases production. cost, and there is still a risk of contamination by residual organic matter

[0005] In addition, the traditional digestion method simply separates the cells from the carrier or directly transfers the cells. The whole process only relies on technicians to manually operate the digester and empirically judge whether the digestion process is complete, which not only causes a large loss of cells during the digestion process, but also And the results are difficult to repeat from batch to batch

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