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An internal reference gene related to the expression and regulation of stress-resistant genes in poplar and its application

An internal reference gene and gene expression technology, applied in the field of genetic engineering, can solve the problem of low stability and achieve the effect of high stability

Active Publication Date: 2019-06-07
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the researchers found that in red clover (Trifolium pratense) leaves, the expression of UBC2 and UBQ10 is the most stable; in the stem, the expression of UBC2 and YLS8 is relatively stable; in the root, the expression of EIF-4a and UBC2 is stable; and in various tissues In , the stability of GAPDH and SAND is low
In addition, traditional housekeeping genes such as Actin, ubiquitin, 18S rRNA, EF1α, TUB, and TUA have been applied to poplar real-time fluorescent quantitative PCR analysis, however, these genes still have many shortcomings in terms of systematic screening (Pettengill et al., 2012)

Method used

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  • An internal reference gene related to the expression and regulation of stress-resistant genes in poplar and its application
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  • An internal reference gene related to the expression and regulation of stress-resistant genes in poplar and its application

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Embodiment 1

[0022] 1. Materials

[0023] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.

[0024] 2. Method

[0025] 2.1 Screening of internal reference genes

[0026] 2.1.1 Collect the gene chips related to the expression of poplar stress-resistant genes, select the gene chips with numbers GSE13990, GSE15242, GSE21171 and GSE23637 as specific screening chips; the gene chip data of GSE13990, GSE15242, GSE21171 and GSE23637 are from https: / / www .ncbi.nlm.nih.gov / gds / ? term = directly downloaded from the website.

[0027] 2.1.2 Use ExpressionConsle, a normalized gene chip data processing software developed by Affiliate, USA, to normalize the probes contained in the 4 gene chips. The steps include:

[0028] Step 1: Use the MAS5 algorithm that comes with the Expression Consle software to calculate the P-Value ...

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Abstract

The invention discloses a reference gene related to stress resistance gene expression regulation of populus trichocarpa and application thereof. The reference gene has a nucleotide sequence as shown in SEQ ID NO.1 which is shown in the description. Compared with a house-keeping gene of the populus trichocarpa, the reference gene disclosed by the invention has higher stability under abiotic stress, is more ideal and is related to the stress resistance gene expression regulation of the populus trichocarpa; the reference gene has an important significance in research on the expression level of the stress resistance gene of the populus trichocarpa and systematic stress resistance research on the populus trichocarpa, and has an important guiding function for further breeding high-quality stress-resisting plants.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to an internal reference gene related to the regulation of the expression and regulation of stress resistance genes of poplars and its application. Background technique [0002] Poplar (Populus trichocarpa) is an important green energy tree species, vigorously developing the poplar industry is of great benefit to the abundance of forest resources and the improvement of the natural ecological environment. Poplars are mainly distributed in arid and semi-arid regions such as North China and Northwest my country in China. Drought will not only affect the photosynthetic rate of leaves, growth rate of branches and dry matter accumulation, but also reduce the dry weight and volume of poplars, thus restricting the growth of poplars. Fast-growing and high-yield. Drought is often associated with osmotic stress caused by high salinity, affecting the growth and yield of poplar. As ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12Q1/6895C12Q1/6851
CPCC07K14/415C12Q1/6851C12Q1/6895C12Q2600/13C12Q2600/158C12Q2600/166C12Q2531/113C12Q2545/101C12Q2563/107
Inventor 严涵薇项艳陈竹刘欢龙
Owner ANHUI AGRICULTURAL UNIVERSITY
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