Swine-origin escherichia coli isolating and identifying method

An identification method and Escherichia coli technology, applied in the field of microorganisms, can solve the problems of ambiguous colony shape, unfavorable picking and separation, inaccurate identification results, etc., and achieve the effects of clear shape, easy picking and separation, and low cost.

Active Publication Date: 2017-05-31
LUOYANG HUIDE BIO ENG CO LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the identification result of this method is not accurate enough, especially the colony shape is blurred in the separation and purification operation, which is not conducive to picking and separating

Method used

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  • Swine-origin escherichia coli isolating and identifying method
  • Swine-origin escherichia coli isolating and identifying method

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0023] The method for isolating and identifying swine-derived Escherichia coli comprises the following steps:

[0024] 1) Collection of samples: 40 samples of excrement from unweaned piglets with diarrhea were collected from a large-scale pig farm in a certain county;

[0025] 2) Bacterial isolation and culture: Inoculate the collected pig feces samples into nutrient broth medium respectively, place them on a shaker and culture them at 37°C and 150 rpm for 18 hours, and pick a small amount of bacterial liquid with a sterile inoculation stick to mark Inoculate on MacConkey agar medium, incubate in a 37°C incubator for 18 hours, observe the growth of the colonies, and pick pink, single or paired colonies and inoculate them on eosin-methylene blue agar medium, at 37°C Cultivate in the incubator for 18 hours, observe the growth of the colonies, pick purple-black, single or paired colonies for use, and isolate 17 strains of bacteria from 40 samples collected;

[0026] The composit...

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Abstract

The invention discloses a swine-origin escherichia coli isolating and identifying method and belongs to the technical field of microorganisms. The method mainly comprises the three steps of bacteria isolated culture, gram staining and microscopic examination and biochemical identification testing. The method is simple, low in time consumption and low in cost, and an identification result is accurate and reliable. Bacteria isolated culture is conducted in a selective mode by adopting a nutrient broth culture medium, a MacConkey agar culture medium and an eosin-methylene blue agar culture medium in sequence, and a grown bacterial colony has a clear morphology and can be selected and separated conveniently.

Description

technical field [0001] The invention relates to a method for isolating and identifying porcine Escherichia coli, belonging to the technical field of microbes. Background technique [0002] Escherichia coli is a kind of zoonotic pathogen that widely exists in nature and can cause co-infection of humans and animals. According to its pathogenic mechanism, it can be divided into three types: commensal, enteropathogenic and extraenteropathogenic. Escherichia coli is an acute, pleomorphic, intestinal infectious disease mainly caused by pathogenic Escherichia coli. In veterinary clinics, due to differences in the serotype, immune status, physiological function, and age of pigs, the common porcine colibacillosis is divided into three types, namely, yellow diarrhea of ​​3-day-old piglets, 7-10 Pullorum in day-old piglets and edema disease in 30-day-old piglets. Porcine colibacillosis occurs all over the world, and piglet yellow and pullorum are the most common infectious diseases i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/10C12N1/02C12R1/19
CPCC12N1/02C12Q1/10
Inventor 董发明张炳亮白喜婷梁立钦王文文杨国栋邱妍
Owner LUOYANG HUIDE BIO ENG CO LTD
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