Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit for detecting susceptibility of cervical cancer, and SNP marker of kit

A marker and susceptibility technology, applied in the field of genetic testing, can solve problems such as lack of system integration, limited detection range, and limitations, and achieve the effects of good technical reproducibility, high cost performance, and high practicability

Inactive Publication Date: 2017-05-31
深圳市核子基因科技有限公司
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, studies have discovered a large number of genetic markers associated with various common diseases. However, due to the lack of sufficient sensitivity and extensive (high-throughput detection sites, high-throughput detection samples) screening for multiple disease-related markers methods of investigation and testing, making the genetic markers of these common diseases not widely available
In addition, the current lack of systematic and effective integration of genetic markers for common diseases greatly restricts the development of early prevention, diagnosis and treatment of common diseases
Existing tests only detect genetic markers for a single disease or a group of diseases, and the detection range is limited, and there is no early detection method for some common diseases
[0005] At present, some traditional medical methods, such as tissue cell detection, have their inherent defects. Improper sampling location, insufficient tissue cell sample materials, or lack of experience will lead to misdiagnosis of cervical cancer.
Although other technologies such as imaging have been widely used in the examination and diagnosis of cervical cancer, they still have great limitations in the qualitative determination of the degree of cervical cancer.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for detecting susceptibility of cervical cancer, and SNP marker of kit
  • Kit for detecting susceptibility of cervical cancer, and SNP marker of kit
  • Kit for detecting susceptibility of cervical cancer, and SNP marker of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Used to detect SNP sites related to cervical cancer susceptibility

[0036] Among them, rs16945692, rs7213430 and rs4968451 are located in the BRIP1 region of the gene, rs9344 is located in the CCND1 region of the gene, rs79190729 is located in the CD28 region of the gene, rs1801270 is located in the CDKN1A region of the gene, rs16997517 is located in the CSF2RB region of the gene, rs1126497 is located in the EPCAM region of the gene, rs1047840 is located in the 171 region of the gene EXO Gene EXOC1 region, rs3212227 in the gene IL12B region, rs2275913 in the gene IL17 region, rs1801275 in the gene IL4R region, rs28493229 in the gene ITPKC region, rs2736098 in the gene TERT region, rs16970849 in the gene TMC8 region, rs1799724 in the gene TNFα region, rs22909RC07 in the gene TN region region, rs8067378 is located in the gene ZPBP2 region.

Embodiment 2

[0037] Example 2 Kit for detecting susceptibility to cervical cancer

[0038] 1. Preparation of the kit:

[0039] 1. Design and synthesize PCR amplification primers and single-base extension primers for the 19 SNP sites. The PCR amplification primers and single base extension primers of the SNP sites to be tested are shown in Table 1

[0040] Table 1 PCR amplification primers and single-base extension primers for SNP sites to be tested

[0041]

[0042]

[0043] 2. The kit also includes Taq enzyme, dNTP mixture, MgCl 2 Solution, PCR reaction buffer, enzyme digestion reaction buffer, deionized water.

[0044] 2. The detection method of the kit:

[0045] 1. Extraction of DNA

[0046] 1.1 DNA extraction from oral swab

[0047] 1) Put the oral swab in a 2ml centrifuge tube and add 400μl PBS.

[0048] 2) Add 20 μl QIAGEN Protease and 400 μl Buffer AL. Immediately vortex for 15s to mix. To ensure efficient lysis, sample and Buffer AL must be mixed immediately and thor...

Embodiment 3

[0094] Example 3 Literature cited by 19 SNP sites closely related to cervical cancer

[0095] Table 5 Literature cited by 19 SNP sites closely related to cervical cancer

[0096]

[0097]

[0098]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an SNP marker for detecting susceptibility of cervical cancer. The SNP marker comprises 19 SNP sites including rs16945692, rs7213430, rs4968451, rs9344, rs79190729, rs1801270, rs16997517, rs1126497, rs1047840, rs13117307, rs3212227, rs2275913, rs1801275, rs28493229, rs2736098, rs16970849, rs1799724, rs2290907and rs8067378. The invention further discloses a PCR amplification primer of the SNP marker and a single-base extension primer and a kit thereof. The 19 SNP sites can provide important basis for the disease risk evaluation, and diagnosis reference of cervical cancer.

Description

technical field [0001] The invention belongs to the field of genetic detection, in particular to a kit for detecting susceptibility to cervical cancer and its SNP marker. Background technique [0002] With the development of society and economy, cervical cancer, a malignant disease that endangers the majority of women, has attracted more and more attention from all walks of life. As a malignant tumor, cervical cancer has a relatively complex pathogenesis, which is the result of the interaction between genetics and the environment. Among them, genetic factors play an important role in the occurrence of cervical cancer, and the genetic material basis of individual differences One is single nucleotide polymorphisms. [0003] The association analysis method using single nucleotide polymorphism (Single Nucleotide Polymorphsm, SNP) as a genomic marker is currently one of the most commonly used methods for detecting genetic susceptibility genes of diseases. SNP refers to the DNA ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/156Y02A50/30
Inventor 张核子
Owner 深圳市核子基因科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com