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Marker for monitoring disease progression of gliocytoma

A glioblastoma and material technology, applied in the field of markers for monitoring the progress of glioblastoma, can solve the problem that the prognosis of glioblastoma patients has not been significantly improved

Inactive Publication Date: 2017-05-31
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although some progress has been made in multiple treatments in recent years, the prognosis of glioma patients has not improved significantly, and the average survival time of GBM patients is still less than 14 months

Method used

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  • Marker for monitoring disease progression of gliocytoma
  • Marker for monitoring disease progression of gliocytoma
  • Marker for monitoring disease progression of gliocytoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, detect the primer design of DILC gene expression level

[0063] Download the nucleic acid sequence of the long non-coding RNA DILC in GeneBank, select the DNA molecule shown in Sequence 1 (152-393 of the DILC gene) as the target sequence for real-time quantitative PCR, design and synthesize the DILC gene for real-time quantitative PCR detection Sequence-specific primers, the primer sequences are as follows:

[0064] Upstream primer: f1:5'-CAGAACCTTAGTCCCGGGAA-3' (SEQ ID No.2);

[0065] Downstream primer: r1:5'-CGTCTGCCACTTCGTTGTAG-3' (SEQ ID No. 3).

Embodiment 2

[0066] Example 2, the application of DILC gene as a marker of glioblastoma disease progression

[0067] 1. Detection of DILC gene expression level by real-time quantitative PCR

[0068] 1. Glioblastoma case and control samples

[0069] All 43 cases of glioblastoma were donated by the Second Hospital of Hebei Medical University, China. According to the 2007 World Health Organization (WHO) pathological grading standard, 43 cases of glioblastoma were grouped as follows: I / II grade, 23 cases; III grade, 7 cases; IV grade, 6 cases. Tissue samples from 43 glioblastoma cases collected from medical donations were obtained after approval by the local medical ethics committee, and were stored in liquid nitrogen for use in experiments.

[0070] A total of 7 cases of normal control samples.

[0071] 2. Acquisition of cDNA

[0072] (1) Extraction of RNA

[0073] Grind the clinical tissue samples from step 1 to extract RNA. For the extraction method of RNA, please refer to the instruc...

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Abstract

The invention discloses a marker for monitoring disease progression of gliocytoma. According to the clinic grouping results in stages, A test proves that the expression levels of DILC genes of I / II stage, III stage and IV stage gliocytoma patients are obviously lower than that of a normal control group, the expression levels of DILC genes of III stage and IV stage gliocytoma sets are obviously lower than that of the I / II stage set and the difference is obvious. The intimate level between the expression level of the DILC gene and the disease progression of gliocytoma is high and has an obvious negative relationship, namely, the lower the expression level of the DILC is, the higher the level of the gliocytoma is. A primer used for detecting the expression level of the DILC gene, provided by the invention, is excellent in specificity; the detection method is accurate and quick; the marker can be used for clinically diagnosing or detecting the disease progression of gliocytoma.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a marker for monitoring the progression of glioma. Background technique [0002] Glioma is the most prevalent and fatal brain tumor of the central nervous system in adults. According to the pathological and clinical characteristics, it can be divided into poles I to IV, among which glioblastoma multiforme (GBM) is the most malignant subtype of glioblastoma. Although some progress has been made in multiple treatments in recent years, the prognosis of glioma patients has not been significantly improved, and the average survival time of GBM patients is still less than 14 months. [0003] With the development of genome-wide and transcriptome sequencing technologies, researchers have discovered a large number of long non-coding RNAs (LncRNAs), and the research on LncRNAs has attracted more and more attention. LncRNAs are nonprotein-coding transcripts that play an important r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 夏文戎徐东刚王乐朋扈玉华付文亮
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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