Primer and kit for quickly detecting transgenic maize PAT
A technology of genetically modified corn and kits, applied in recombinant DNA technology, biochemical equipment and methods, and microbial determination/inspection, etc., can solve problems such as cumbersome operations, threats to the health of scientific researchers, pollution, etc. The test results are objective and the effect of avoiding cross-contamination
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experiment example 1
[0060] The positive amplification products were sequenced and verified by sequencing and other methods. When the positive amplification product sequences were compared by Blast, the sequences were all highly homologous to the Genbank target sequence; Sensitivity experiments were performed on the standard.
[0061] The results show that the detection threshold of the present invention reaches 0.1%, repeated experiments show that the detection sample has good repeatability, and there is also a good linear relationship between the logarithmic value of the diluted template concentration and the Cp value. 2 ≥0.96. It shows that the method has good precision and good stability.
experiment example 2
[0063] In order to verify the accuracy and efficiency of using the method and kit of the present invention to detect transgenic corn; we use traditional PCR and the method of the present invention to detect the same batch of transgenic corn, and each process detects 100 samples to be tested. Each treatment was repeated 3 times, and the results were averaged; the last two test results were compared with the results of next-generation sequencing respectively, and the accuracy rate of each method was compared. The test results are shown in Table 1;
[0064] Table 1
[0065] corn variety Detection method of the present invention Traditional PCR Huanong 866 97.9% 84.8% Shenyu No. 17 98.1% 83.9%
[0066] The results of Experimental Example 2 show that the detection method of the present invention has a higher accuracy of detection results, up to 98%, and the accuracy of traditional PCR detection results is about 84%.
[0067] The results of comprehen...
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