Primer and kit for quickly detecting transgenic maize PAT

Abstract

The invention specifically discloses a primer and a kit for quickly detecting transgenic maize PAT. The primer comprises an inner reference primer pair and an exogenous gene primer pair; the primer pair in the kit comprises the inner reference primer pair and the exogenous gene primer pair; a reaction system component in the kit comprises HRM reaction premixed solution, the inner reference primer pair, the exogenous gene primer pair, a reaction template and deionized water. The primer is reasonable in component and proportion, is quick and convenient in detection, is high in accuracy and is suitable for fluorescence PCR detection for Zein gene and PAT gene of transgenic sorghum. The detection sensitivity of the primer is higher and can reach up to 0.1%; the stability is higher; the logarithm value of template concentration and the Cp value have an excellent linear relation: R2 more than or equal to 0.96; the accuracy of the detection result of the primer is as high as about 98%; the operation step is simplified according to the invention, so that the detection period is shortened, the quantitative analysis can be realized and the application value is higher.

Description

technical field [0001] The invention relates to the technical field of rapid detection of transgenic plants, in particular to a primer and a kit for rapid detection of transgenic corn PAT. Background technique [0002] Corn is one of the most important food crops in China. It occupies an important position in my country's agricultural production and is also a major contributor to ensuring my country's food security. Although genetically modified corn has not been approved for production in my country, relevant scientific researchers have achieved many results in genetically modified corn breeding. Of course, it is unavoidable that genetically modified corn will flow into the market through illegal channels. [0003] At present, my country's entry-exit inspection and quarantine industry standard SN / T1196-2003 "Qualitative PCR detection method for genetically modified components of corn seeds" stipulates the qualitative detection of genetically modified components in corn MON8...

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Problems solved by technology

Although genetically modified corn has not been approved for production in my country, relevant scientific researchers have achieved many results in corn genetically modified breeding. Of course, it is unavoidable that genetically modified corn enters the market through illegal channels

[0003] At present, my country's entry-exit inspection and quarantine industry standard SN / T1196-2003 "Qualitative PCR detection method for genetically modified components of corn seeds" stipulates the qualitative detection of genetically modified components in corn MON810, Bt11, Evebt176, T14 / T25, CBH351, GA21 lines, Most of these gene detection methods use conventional PCR methods, but conventional PCR methods require electrophoresis after PCR amplification, which is not only cumbersome to operate, but also easily causes pollution, and also requires the use of fluorescent dyes that may cause cancer. good health

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