A method for shielding protein in bacterial leaching solution to improve the sensitivity of the electrode to measure arsenic
A technology for leachate and protein, which is used in measuring devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of reduced sensitivity, inaccurate arsenic measurement by ion electrode, and high protein concentration, and achieve the effect of improving sensitivity.
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Embodiment 1
[0023] A method for shielding proteins in bacterial leachate to improve the sensitivity of an electrode to measure arsenic, wherein the bacterial leachate is the leachate in the process of bacterial oxidation pretreatment of high-sulfur arsenic-containing gold ore, and the method comprises the following steps:
[0024] (1) Take 1mL of the bacterial extract to be tested and add 5mL of concentrated sulfuric acid, 5% CuSO 4 Solution 0.25mL, 0.5g hydrazine sulfate, after heating at 200°C for 2.0 h, perform protein shielding;
[0025] (2) Cool the bacterial mixture to be tested that shields the protein to room temperature, add 60 mL of deionized water and boil for 5 min, and determine the protein concentration to be 1.1×10 -5 g·L -1 ;
[0026] (3) Use the iodine ion electrode to measure the total arsenic content in the boiled bacterial mixture. The specific method is: take four 100mL volumetric flasks, and add 1mol L -1 KNO 3 Solution 5 mL, 0.5mol L -1 C 2 h 3 ClO 2 Solut...
Embodiment 2
[0031] A method for shielding proteins in a bacterial leachate to improve the sensitivity of an electrode for measuring arsenic, wherein the bacterial leachate is the leachate in the process of bacterial oxidation pretreatment of arsenic-containing refractory gold ore, and the method comprises the following steps:
[0032] (1) Take 1mL of the bacterial extract to be tested and add 4mL of concentrated sulfuric acid, 5% CuSO 4 Solution 0.2mL, 0.4g hydrazine sulfate, after heating at 300°C for 1.5 h, perform protein shielding;
[0033] (2) Cool the bacterial mixture to be tested that shields the protein to room temperature, add 50 mL of deionized water and boil for 4 minutes, and determine the protein concentration to be 2.4×10 -5 g·L -1 ;
[0034] (3) Use the iodine ion electrode to measure the total arsenic content in the boiled bacterial mixture solution. The specific method is: add 1 mol L to 1 mL of the boiled bacterial mixture solution. -1 KNO 3 Solution 3mL, 0.5mol·L...
Embodiment 3
[0039] A method for shielding proteins in a bacterial leachate to improve the sensitivity of an electrode to measure arsenic, the bacterial leachate is the leachate in the process of bacterial oxidation pretreatment of complex and refractory gold ores containing arsenic, and the method comprises the following steps:
[0040] (1) Take 1mL of the bacterial extract to be tested and add 3mL of concentrated sulfuric acid, 5% CuSO 4 Solution 0.15mL, 0.3g hydrazine sulfate, after heating at 350°C for 1.0 h, perform protein shielding;
[0041] (2) Cool the bacterial mixture to be tested that shields the protein to room temperature, add 40 mL of deionized water and boil for 3 minutes, and determine the protein concentration to be 3.1×10 -5 g·L -1 ;
[0042] (3) Use the iodine ion electrode to measure the total arsenic content in the boiled bacterial mixture solution. The specific method is: add 1 mol L to 1 mL of the boiled bacterial mixture solution. -1 KNO 3 Solution 5mL, 0.5mo...
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