Preparation method of nano-hydroxyapatite-doped porous bionic bone scaffold

A technology of nano-hydroxyapatite and biomimetic bone, which is applied in the field of preparation of porous biomimetic bone scaffolds, can solve the problems of adverse effects and refractory degradation, and achieve pore penetration, good biocompatibility, good bioactivity and osteoconduction Effect

Inactive Publication Date: 2017-06-09
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantage is that it is difficult to degrade, and t

Method used

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  • Preparation method of nano-hydroxyapatite-doped porous bionic bone scaffold
  • Preparation method of nano-hydroxyapatite-doped porous bionic bone scaffold
  • Preparation method of nano-hydroxyapatite-doped porous bionic bone scaffold

Examples

Experimental program
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Example Embodiment

[0033] Example 1:

[0034] Preparation of porous hyaluronic acid (5mg / ml)-gelatin (5mg / ml) composite scaffold doped with nano-hydroxyapatite:

[0035] In a 60℃ water bath, dissolve hyaluronic acid and gelatin in three-distilled water to prepare a 5mg / ml hyaluronic acid solution and a 5mg / ml gelatin mixture; stir to remove bubbles, mix the above two solutions, and configure the volume ratio 1:9 and 9:1 hyaluronic acid-gelatin solution;

[0036] After ultrasonic dispersion treatment of nano-hydroxyapatite, it is added to the above solutions according to different mass ratios (one of the solutions does not add nano-hydroxyapatite) to form a hyaluronic acid-gelatin suspension without nano-hydroxyapatite, Hyaluronic acid-gelatin suspension containing 1mg / ml nano-hydroxyapatite and 50mg / ml nano-hydroxyapatite;

[0037] The hyaluronic acid-gelatin, hyaluronic acid-gelatin-nano-hydroxyapatite mixture is allowed to stand for defoaming, injected into a mold, pre-frozen at -80°C for more than 3...

Example Embodiment

[0039] Example 2:

[0040] Preparation of porous hyaluronic acid (15mg / ml)-gelatin (0.2g / ml) composite scaffold doped with nano-hydroxyapatite:

[0041] In a 60℃ water bath, dissolve hyaluronic acid and gelatin in three-distilled water to prepare a 15mg / ml hyaluronic acid solution and a 0.2g / ml gelatin mixture; stir to remove bubbles, mix the above two solutions, and prepare the volume Hyaluronic acid-gelatin solutions with ratios of 1:9 and 9:1;

[0042] After ultrasonic dispersion treatment of nano-hydroxyapatite, it is added to the above solutions according to different mass ratios (one of the solutions does not add nano-hydroxyapatite) to form a hyaluronic acid-gelatin suspension without nano-hydroxyapatite, Hyaluronic acid-gelatin suspension containing 1mg / ml nano-hydroxyapatite and 50mg / ml nano-hydroxyapatite;

[0043] The hyaluronic acid-gelatin, hyaluronic acid-gelatin-nano-hydroxyapatite mixture is allowed to stand for defoaming, injected into a mold, pre-frozen at -80°C for...

Example Embodiment

[0046] Example 3:

[0047] Cell culture, analysis and evaluation of porous bionic bone scaffold doped with nano-hydroxyapatite:

[0048] The composite stent designed and prepared by the laboratory was sterilized. The stent was immersed in 75% ethanol for 3 hours, irradiated with ultraviolet for 1 hour, and immersed in PBS containing 10% bi-antibody for deep sterilization. After surface modification with type I collagen, the above 9 groups of composite scaffolds were inoculated with osteogenic precursor cells at a density of 2×10 6 Cells / mL, after 6 hours of cell attachment, add culture medium. After cells are cultured for 7 days, perform cell death, apoptosis and other related staining, cell proliferation and differentiation detection, and Image Pro software analysis. by Figure 5 It can be seen that osteoblast precursor cells are distributed on the pores and surface of the scaffold in a cluster or single cell state, and the cells are connected to the scaffold, and between cells an...

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Abstract

The invention provides a preparation method of a nano-hydroxyapatite-doped porous bionic bone scaffold. According to the preparation method, a hyaluronic acid-gelatin-nano-hydroxyapatite cross-linked composite porous scaffold with good mechanical strength is prepared from hyaluronic acid, gelatin and nano-hydroxyapatite by virtue of a freeze-drying method and a chemical cross-linking method. The porous composite scaffold prepared by virtue of the preparation method has uniform aperture and very good cell adhesion capacity and mechanical strength and further has osteoconductivity, hole walls are thin, and pore spaces are penetrated. The preparation method has the advantages that the composite scaffold can be used for relatively truly simulating a bone tissue microenvironment, and parameters of aperture, porosity, degradation rate, water absorption rate, mechanical strength and the like of the scaffold can be adjusted and controlled by adjusting the concentration, volume ratio and freeze drying conditions of each component; the adopted materials have good biocompatibility, and the prepared scaffold can meet an internal porous structure of bone tissues; and the sources of the material are sufficient, and the preparation process is simple.

Description

[0001] Technical field: [0002] The invention relates to the technical field of novel biomaterials and tissue engineering, in particular to a method for preparing a porous bionic bone scaffold doped with nano-hydroxyapatite. [0003] Background technique: [0004] The present invention is based on tissue engineering. Bone tissue engineering is one of the effective ways to repair bone defects clinically, and has attracted extensive attention from scholars at home and abroad in recent years. The three or four elements of bone tissue engineering mainly include scaffold materials, seed cells, the integration of cells and scaffold materials, and the integration of implants and in vivo microenvironment. The process refers to the expansion and culture of seed cells in vitro, and then The amplified cells are transplanted to a scaffold material with good biocompatibility and degradability to continue culturing, so that the cells adhere to the scaffold to form a cell-scaffold complex; ...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/24A61L27/12A61L27/56C08J9/36
CPCA61L27/20A61L27/12A61L27/24A61L27/56A61L2300/112A61L2300/232A61L2300/252A61L2430/02C08J9/36C08J2305/08C08J2389/00C08J2405/08C08J2489/00C08L5/08
Inventor 秦建华张晓庆
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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