Method for inducing siniperca chuats artificial gynogenesis with heterogenous sperms
A technology for gynogenesis and gynogenesis of mandarin fish is applied in the field of artificial gynogenesis induced by heterologous spermatozoa, which can solve the problems of high cost and time-consuming, and achieve significant economic benefits, good genetic and breeding value, and prospect of popularization and application. Effect
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[0026] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.
[0027] A method for inducing artificial gynogenesis of mandarin mandarin fish with heterologous sperm, the main steps of which are as follows:
[0028] S1: Cryopreservation of allogeneic spermatozoa of spotted mandarin fish: the spermatozoa of spotted mandarin fish were stored at low temperature (-24°C) with Hanks freezing diluent, and the storage period was 24 hours. When thawing, thaw the frozen semen tube in a 25°C water bath for later use; the Hanks frozen diluent is prepared from the following raw materials: KCL 0.4g, NaCL 8.00g, NaHCO3 0.35g, KH2PO4 0.06g, Na2HPO4·7H2O 0.09g, Na2HPO4·12H2O0.10g, MgSO4·7H2O 0.10g, MgCL2·6H2O 0.10g, CaCL20.14g, Glucose 1.00g; add distilled water to make up to 1 liter;
[0029] S2: Genetic inactivation of Siniperca semen: The thawed Siniperca semen is diluted with Hanks freezing solution at a ratio o...
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