Method for inducing siniperca chuats artificial gynogenesis with heterogenous sperms

A technology for gynogenesis and gynogenesis of mandarin fish is applied in the field of artificial gynogenesis induced by heterologous spermatozoa, which can solve the problems of high cost and time-consuming, and achieve significant economic benefits, good genetic and breeding value, and prospect of popularization and application. Effect

Active Publication Date: 2017-06-13
武汉市农业科学技术研究院水产科学研究所 +1
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Problems solved by technology

[0004] In the process of traditional aquatic animal breeding, the construction of pure lines by continuous inbre

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  • Method for inducing siniperca chuats artificial gynogenesis with heterogenous sperms
  • Method for inducing siniperca chuats artificial gynogenesis with heterogenous sperms
  • Method for inducing siniperca chuats artificial gynogenesis with heterogenous sperms

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Embodiment Construction

[0026] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0027] A method for inducing artificial gynogenesis of mandarin mandarin fish with heterologous sperm, the main steps of which are as follows:

[0028] S1: Cryopreservation of allogeneic spermatozoa of spotted mandarin fish: the spermatozoa of spotted mandarin fish were stored at low temperature (-24°C) with Hanks freezing diluent, and the storage period was 24 hours. When thawing, thaw the frozen semen tube in a 25°C water bath for later use; the Hanks frozen diluent is prepared from the following raw materials: KCL 0.4g, NaCL 8.00g, NaHCO3 0.35g, KH2PO4 0.06g, Na2HPO4·7H2O 0.09g, Na2HPO4·12H2O0.10g, MgSO4·7H2O 0.10g, MgCL2·6H2O 0.10g, CaCL20.14g, Glucose 1.00g; add distilled water to make up to 1 liter;

[0029] S2: Genetic inactivation of Siniperca semen: The thawed Siniperca semen is diluted with Hanks freezing solution at a ratio o...

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Abstract

The invention discloses a method for inducing siniperca chuats artificial gynogenesis with heterogenous sperms. The method comprises the steps that siniperca scherzeri seminal fluid is taken and freeze-preserved with sperm preservation liquid and then is irradiated by ultraviolet light after being diluted, and sperms are subjected to genetic inactivation; the inactivated sperms and siniperca chuats eggs are subjected to mixed fertilization, and egg gynogenesis is started; the eggs are transferred into fresh water for cold shock treatment after being fertilized, genomes are doubled, and heterogenous gynogenesis diploid is generated; the siniperca chuats gynogenesis diploid is transferred into culture and incubation water, incubation is carried out at room temperature, management is carried out according to a common siniperca chuats fry culture method, and siniperca chuats gynogenesis diploid fry are obtained. The siniperca chuats offspring generated through inducing of the method can accelerate excellent gene homozygosis, excellent inheritable characters can be fast fixed, and the disease resisting capacity is remarkably enhanced. According to the method, the conditions are easy to obtain, full-female siniperca chuats groups with the growth speed about 20% higher than that of common male siniperca chuats can be obtained later, and high breeding potential and application value are achieved.

Description

technical field [0001] The invention relates to the field of fish genetics and breeding, in particular to a method for inducing artificial gynogenesis of mandarin fish with heterologous sperm. Background technique [0002] Siniperca chuatsi belongs to the order Perciformes, Serranidae, Sinipercinae, and Siniperca. It is a rare freshwater fish in my country. It is rich in 8 kinds of amino acids needed by the human body, and has extremely high nutritional value and therapeutic effect. The mandarin fish feeds on live bait of other fishes throughout its life. Among the several species of mandarin fish in the genus Siniperca (such as big-eyed mandarin fish, spotted mandarin mandarin fish, and cocked-mouthed mandarin fish), the mandarin mandarin fish with cocked mouth is the largest in size and grows the fastest, so The cocked mandarin fish is the most important species in the aquaculture industry of mandarin fish in my country. In the following statements of this project, unless ...

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Application Information

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IPC IPC(8): A01K61/10
CPCA01K61/00Y02A40/81
Inventor 杨凯童金苟成为为俞小牧朱思华李波付北德程颖红邓国乔谢德斌
Owner 武汉市农业科学技术研究院水产科学研究所
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