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A kind of sulfate-reducing bacteria activator and its application in microbial oil recovery

A microbial oil recovery and sulfate technology, applied in the field of oil recovery, can solve problems such as the hazards of the reinjection water system and corrosion of oilfield pipelines, and achieve the effects of rapid directional activation, rapid activation, and enhanced biochemical activity

Active Publication Date: 2019-12-10
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sulfate-reducing bacteria are ubiquitous in oilfield operations and multiply in large numbers, which has brought serious harm to the reinjection water system of the oilfield and is one of the main causes of oilfield pipeline corrosion

Method used

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  • A kind of sulfate-reducing bacteria activator and its application in microbial oil recovery
  • A kind of sulfate-reducing bacteria activator and its application in microbial oil recovery
  • A kind of sulfate-reducing bacteria activator and its application in microbial oil recovery

Examples

Experimental program
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Effect test

Embodiment 1

[0043] This implementation provides a kind of sulfate-reducing bacteria activator (formula 1), wherein, in mass / volume ratio, every liter of sulfate-reducing bacteria activator comprises the following components:

[0044] 0.6% molasses, 0.4% yeast powder, 0.2% peptone, 0.2% Tween 80, 0.5% K 2 HPO 4 , 0.2% NaCl, 0.2% MgSO 4 , 0.10% FeCl 3 and the remainder of the water.

Embodiment 2

[0060] This implementation provides a kind of sulfate-reducing bacteria freeze-dried powder, and the specific preparation steps are as follows:

[0061] a. formula 1 that sulfate-reducing bacteria bacterial strain adopts embodiment 1 to carry out enrichment culture, in 13 days, cultivate to optimal state (by OD 600nm The detection is between 1.2-1.8); then the cells are extracted and made into a suspension with water;

[0062] b. Put the above suspension into a -86°C refrigerator, and freeze it at ultra-low temperature for more than 48 hours;

[0063] c. Put the frozen cells into a Labconco lyophilizer, and dry them at low temperature for 18-24 hours to obtain lyophilized powder of sulfate-reducing bacteria.

Embodiment 3

[0068] The present embodiment provides the indoor oil displacement experiment of sulfate reducing bacteria, specifically as follows:

[0069] (1) Oil displacement ability of sulfate reducing bacteria

[0070] In order to confirm the oil displacement ability of sulfate reducing bacteria, the oil displacement experiment of injecting fresh bacteria was carried out first, and the specific steps are as follows:

[0071] a. Cultivate the sulfate-reducing bacteria strains with ATCC 1249 medium for about 28 days, then add sterilized deionized water and fully mix them into a bacterial suspension;

[0072] b Inject the bacterial suspension into the rock core after water flooding, and statically revive for 48 hours;

[0073] c. Carry out post-water flooding experiments.

[0074] After testing, injecting 0.5PV (void volume of 0.5 cores) with the enriched cultured sulfate-reducing bacteria solution can increase the recovery rate by 11.78% on the basis of water flooding. Very obvious (te...

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Abstract

The invention provides an activator for a sulfate reducing bacterium and application thereof to microbial oil extraction, wherein every liter of the activator for the sulfate reducing bacterium is prepared from the following components metered according to a mass / volume ratio: 0.3 to 0.8 percent of molasses, 0.3 to 0.5 percent of yeast powder, 0.2 to 0.4 percent of peptone, 0.1 to 0.2 percent of tween 80, 0.3 to 0.5 percent of K2HPO4, 0.1 to 0.2 percent of NaCl, 0.2 to 0.3 percent of MgSO4, 0.008 to 0.15 percent of FeCl3 and the balance of water. According to the activator and the application thereof, sulfate reducing bacterium freeze-dried powder is made by utilizing the activator for the sulfate reducing bacterium; on the basis, the sulfate reducing bacterium freeze-dried powder is successfully applied to the microbial oil extraction.

Description

technical field [0001] The invention belongs to the technical field of oil recovery, and in particular relates to an activator for sulfate-reducing bacteria and its application in microbial oil recovery. Background technique [0002] Sulfate-reducing bacteria (SRB) widely exist in oil reservoirs. According to different metabolic physiological characteristics, SRB can be roughly divided into two categories: dissimilatory sulfur-reducing bacteria and dissimilatory sulfate-reducing bacteria. Sulfate-reducing bacteria are ubiquitous in oilfield operations and multiply in large numbers, which has brought serious harm to the reinjection water system of the oilfield and is one of the main causes of oilfield pipeline corrosion. But on the other hand, sulfate-reducing bacteria can degrade heavy alkanes, improve the quality of crude oil, and reduce the viscosity of crude oil. Metabolites of sulfate-reducing bacteria, such as organic acids, alcohols, and biogas (H 2 S) It can be diss...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/38C09K8/582C12R1/01
CPCC09K8/582C12N1/20C12N1/38
Inventor 宋文枫马德胜吴康云魏小芳
Owner PETROCHINA CO LTD
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