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Smart phone-based mobile instant microbiological diagnosis instrument

A technology of smart phones and microorganisms, applied in the direction of instruments, scientific instruments, telephone structures, etc., can solve the problems of technological transformation barriers, difficulty in adapting to environmental protection, and large volume

Inactive Publication Date: 2017-06-20
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microbial commercial detectors have made great progress, but the above-mentioned various microbial detectors have the following two problems: one is that they are relatively large and cannot be portable; the other is complex analysis and measurement operations (requires specialized technical training)
At the same time, there is still a gap between my country's research on commercial instruments for rapid microbial diagnosis and foreign advanced levels, especially portable microbial detection instruments are still relatively weak, and it is difficult to adapt to the growing needs of environmental protection, food safety, and medical hygiene.
There are technical transformation barriers in the commercialization of microbial diagnostic instruments and microbial detection technology in laboratories

Method used

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  • Smart phone-based mobile instant microbiological diagnosis instrument

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Detection of sulfate-reducing bacteria:

[0028] The microorganisms used in the experiment were cultured in suspension in lysed broth (1% peptone, 1% sodium chloride, 0.5% yeast extract, 100 mL water), and a single colony was cultured overnight at 30°C and 200 rpm on a shaking table at 4500 rpm. / Centrifuge for ten minutes, and dilute to different concentrations with PBS buffer solution.

[0029] 100 µL concentration (10 cfu ml -1 ) sulfate-reducing bacteria solution was added to the disposable sulfate-reducing bacteria culture medium, and reacted at 37 °C for 8 hours. Use the excitation light source of the microbial diagnostic instrument to excite the sensor platform to generate light signals, and measure the microbial signals at this concentration.

[0030] 100 µL concentration (10 2 cfu ml -1 ) sulfate-reducing bacteria solution was added to the disposable sulfate-reducing bacteria culture medium, and reacted at 37 °C for 8 hours. Use the excitation light sour...

Embodiment 2

[0039] E. coli detection

[0040] The microorganisms used in the experiment were cultured in suspension in lysed broth (1% peptone, 1% sodium chloride, 0.5% yeast extract, 100 mL water), and a single colony was cultured overnight at 30°C and 200 rpm on a shaking table at 4500 rpm. / Centrifuge for ten minutes, and dilute to different concentrations with PBS buffer solution.

[0041] 100 µL concentration (10 cfu ml -1 ) Escherichia coli bacteria solution was added to the disposable sulfate-reducing bacteria medium, and reacted at 37 °C for 8 hours. Use the excitation light source of the microbial diagnostic instrument to excite the sensor platform to generate light signals, and measure the microbial signals at this concentration.

[0042] 100 µL concentration (10 2 cfu ml -1 ) Escherichia coli bacteria solution was added to the disposable sulfate-reducing bacteria medium, and reacted at 37 °C for 8 hours. Use the excitation light source of the microbial diagnostic instrum...

Embodiment 3

[0051] Staphylococcus aureus detection

[0052] The microorganisms used in the experiment were cultured in suspension in lysed broth (1% peptone, 1% sodium chloride, 0.5% yeast extract, 100 mL water), and a single colony was cultured overnight at 30°C and 200 rpm on a shaking table at 4500 rpm. / Centrifuge for ten minutes, and dilute to different concentrations with PBS buffer solution.

[0053] 100 µL concentration (10 cfu ml -1 ) of Staphylococcus aureus was added to the disposable sulfate-reducing bacteria medium, and reacted at 37 °C for 8 hours. Use the excitation light source of the microbial diagnostic instrument to excite the sensor platform to generate light signals, and measure the microbial signals at this concentration.

[0054] 100 µL concentration (10 2 cfu ml -1 ) of Staphylococcus aureus was added to the disposable sulfate-reducing bacteria medium, and reacted at 37 °C for 8 hours. Use the excitation light source of the microbial diagnostic instrument to...

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Abstract

The invention discloses a smart phone-based microbiological diagnosis instrument. The diagnosis instrument realizes pathogenic microorganism instant detection (Mobile-based Point-of-Care Testing, mPOCT) through combination of microorganism metabolite as a functional unit and a smart phone platform based on microbial selective medium breeding. The functional microorganism metabolite and sensor response material recognition mechanism and reaction kinetics are designed emphatically, a function rule of the recognition responsive functional module in fast microbiological detection and microbiological instant expression and analysis is inspected and cloud service-based smart phone data acquisition and analysis software is designed and researched. The work innovation of the system is revealed in relationship between active small molecule recognition response and microorganisms on a base research level. The diagnosis instrument provides reference to solve on-line, portable and full-automatic electromechanical engineering-biological complex problems of the microbial detection method.

Description

technical field [0001] The invention designs a mobile instant microbial diagnostic instrument based on a smart phone. Background technique [0002] Microorganisms are closely related to our human production and life: on the one hand, they actively participate in the activities of life systems, adjust and promote the dynamic balance of ecosystems, and maintain the vitality and productivity of their environments; on the other hand, some microorganisms seriously threaten human production and life, forcing Humans must be vigilant against it. According to statistics from various countries in the world, the direct annual economic loss caused by microbial infection exceeds 500 billion yuan each year. If effective preventive measures are taken, the loss caused by microorganisms can be reduced by one-third. Therefore, it is of great significance to carry out the research on its detection and identification methods for the construction of the national economy. [0003] In the marin...

Claims

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Application Information

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IPC IPC(8): G01N15/14H04M1/21
CPCG01N15/1431H04M1/21
Inventor 万逸周腾许强刘春胜葛鉴
Owner HAINAN UNIVERSITY