Enzyme-method ethanol detection reagent with high anti-interference capability and high accuracy

Abstract

The invention relates to the technical field of ethanol detection of blood serum and in particular relates to an enzyme-method ethanol (ALC) detection reagent with a high anti-interference capability and high accuracy. A reagent R1 is mainly prepared from a buffering solution, ammonium oxalate, hydrazine hydrochloride, potassium nitroprusside, an ion balancing agent, ascorbic acid oxidase, a protecting agent, a surfactant, NAD (Nicotinamide Adenine Dinucleotide) and a preservative; a reagent R2 is mainly prepared from a buffering solution, a protecting agent, a heavy meal ion chelating agent, alcohol dehydrogenase and a preservative. The ammonium oxalate and the hydrazine hydrochloride are added into the reagent R1 so that interference caused by lactic dehydrogenase and hydroxybutyrate dehydrogenase can be effectively removed; less potassium nitroprusside is added so that interference caused by reducing substances including bilirubin and the like is effectively removed, so that the anti-interference capability of the reagent is greatly improved; the ascorbic acid oxidase is added so that interference caused by ascorbic acid can be effectively removed; the heavy metal ion chelating agent aminotriacetic acid is added so that interference caused by heavy metal ions, especially calcium ions, on dehydrogenase activity is removed very well, so that the activity of the alcohol dehydrogenase is improved, and the reagent becomes one detection reagent with the high anti-interference capability and the high accuracy.

Description

technical field [0001] The invention relates to a serum enzymatic alcohol (ALC) detection reagent with strong anti-interference ability and high accuracy, which belongs to the technical field of clinical in vitro detection. Background technique [0002] Ethanol in the blood is generally ingested by drinking, absorbed by the gastrointestinal tract, enters the blood, and is metabolized and eliminated by the liver at a constant rate. Acetaldehyde is produced during the metabolic process, which can be combined with proteins to form acetaldehyde-protein conjugates, which can directly cause cell damage as cytotoxic substances, and can also be used as antigenic stimuli to produce antibodies. Ethanol also has a direct toxic effect on the body. Long-term excessive drinking is likely to cause diseases such as fatty liver, alcoholic hepatitis, liver cirrhosis, alcoholic pancreatitis, and alcohol-dependent mental disorders. Alcohol consumption by pregnant women can lead to fetal mental...

AI Technical Summary

Problems solved by technology

[0003] At present, there are many methods for detecting blood alcohol. Common ones include early distillation oxidation method, osmotic pressure method, chemical colorimetric method, enzymatic method, homogeneous enzyme immunoassay method, gas chromatography and other detection methods. Distillation oxidation method and osmotic pressure method were used in the early stage, and the accuracy and specificity were poor. Although the homogeneous enzyme immunoassay method and gas chromatography analysis method were high in accuracy, the instruments were expensive, and it was difficult to popularize in general hospitals. With the continuous optimization of hydrogenase extraction technology, the detection method of enzymatic reagents to detect blood ethanol is gradually popularized, and the enzymatic detection of serum ethanol is easy to operate, low in price, and can better cooperate with the use of automatic biochemical analysis instruments. With gradual use, it is found that the reagent is very susceptible to the influence of enzymes such as lactate dehydrogenase and hydroxybutyrate dehydrogenase in the blood. When it is easy, the test result is high, resulting in an increase in false positives. For substances such as urea and ascorbic acid, it is easy Negative interference is generated, which makes the test result low or even negative, which brings big problems to clinical use

Images