Total bile acid detection reagent

Abstract

The invention relates to a total bile acid detection reagent. The detection reagent is composed of the following components: 6-8wt% of a preservative, 30-40mmol / L of sodium pyruvate, 30-40g / L of glycinate buffer solution, 8-10g / L of dextran sulfate, 2-3g / L of 2-hydroxy pyridine-N-oxide, 5-8g / L of a stabilizer, 30-40mmol / L of phosphatic buffer solution, 6000-7000u / L of diaphorase, 3-4g / L of sodium azide, 4-5mmol / L of a chemical oxidant, 2-3wt% of a free-drying protective agent, 30-40mmol / L of NaCl, 6-8KU / L of 3alpha-hydrocarbon sterol dehydrogenase, 3-10g / L of human serum albumin, 6-8mmol / L of an oxidizing auxiliary enzyme I, 12-15g / L of a reduced coenzyme I and 2-3g / L of nitrotetrazolium blue. The detection reagent provided by the invention is high in determination precision, strong in anti-interference capability, good in stability, long in retention period and easy to store and transport.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a detection reagent, in particular to a total bile acid detection reagent. Background technique [0002] The positive rate of serum total bile acid for liver cirrhosis and liver cancer is as high as 95%. The popularization of total bile acid detection has provided a more effective basis for the early diagnosis of various acute and chronic hepatitis, liver cirrhosis, and even liver cancer patients. The clinical diagnosis is of great significance Big. [0003] However, the stability of most total bile acid reagents currently used clinically is relatively poor. The bottle is stable within two weeks (determined value continues to decline); and the negative interference of most brands of total bile acid reagent bilirubin is obvious, and the recovery rate of the reagent is low. The recovery rate of most reagents is about 80%, and some are even as low as 60%. %, so that negative valu...

AI Technical Summary

Problems solved by technology

[0003] However, the stability of most total bile acid reagents currently used clinically is relatively poor. The bottle is stable within two weeks (determined value continues to decline); and the negative interference of most brands of total bile acid reagent bilirubin is obvious, and the recovery rate of the reagent is low. The recovery rate of most reagents is about 80%, and some are even as low as 60%. %, so that clinical samples often have negative values

[0004] At the same time, most of the total bile acid reagents currently used clinically have poor display effects, weak anti-interference ability, and misjudgment may occur

Furthermore, it is easily denatured during transportation, which is not convenient for transportation and storage.