Total bile acid detection reagent

A technology for detecting reagents and total bile acids, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by chemical reactions of materials, etc., can solve the problem of poor display effect of total bile acid reagents, total bile acid reagents Poor stability, inconvenient transportation and storage, etc., to achieve the effect of convenient clinical application, convenient measurement, and stable reagents

Inactive Publication Date: 2017-06-27
徐淼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the stability of most total bile acid reagents currently used clinically is relatively poor. The bottle is stable within two weeks (determined value continues to decline); and the negative interference of most brands of total bile acid reagent bilirubin is obvious, and the recovery rate of the reagent is low. The recovery rate of most reagents is about 80%, and some are even as low as 60%. %, so that clinical samples often have negative values
[0004] At the same time, most of the total bile acid reagents currently used clinically have poor display effects, weak anti-interference ability, and misjudgment may occur
Furthermore, it is easily denatured during transportation, which is not convenient for transportation and storage.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The total bile acid detection reagent of this embodiment is made up of following composition: preservative 6wt%; Sodium pyruvate 30mmol / L; Glycine salt buffer solution 30g / L; Dextran sulfate 8g / L; Stabilizer 5g / L; Phosphate buffer 30mmol / L; Diaphorase 6000u / L; Sodium azide 3g / L; Chemical oxidant 4mmol / L; Lyoprotectant 2wt%; 3α-hydrocarbon steroid dehydrogenase 6KU / L; human serum albumin 3g / L; oxidative auxiliary enzyme I 6mmol / L; reduced auxiliary enzyme I 12g / L; nitrotetrazolium blue 2g / L.

[0018] And, wherein, the preservative is potassium sorbate.

Embodiment 2

[0020] The total bile acid detection reagent of this embodiment is made up of following composition: preservative 8wt%; Sodium pyruvate 40mmol / L; Glycine salt buffer solution 40g / L; Dextran sulfate 10g / L; Stabilizer 8g / L; Phosphate buffer 40mmol / L; Diaphorase 7000u / L; Sodium azide 4g / L; Chemical oxidant 5mmol / L; Lyoprotectant 3wt%; 3α-hydrocarbon steroid dehydrogenase 8KU / L; human serum albumin 10g / L; oxidative auxiliary enzyme I 8mmol / L; reduced auxiliary enzyme I 15g / L; nitrotetrazolium blue 3g / L.

[0021] And, wherein, the preservative is sodium benzoate.

Embodiment 3

[0023] The total bile acid detection reagent of this embodiment is made up of following composition: preservative 7wt%; Sodium pyruvate 35mmol / L; Glycine salt buffer solution 35g / L; Dextran sulfate 9g / L; stabilizer 6.5g / L; phosphate buffer 35mmol / L; diaphorase 6500u / L; sodium azide 3.5g / L; chemical oxidant 4.5mmol / L; lyoprotectant 2.5wt%; NaCl 35mmol / L; 3α-hydrocarbon steroid dehydrogenase 7KU / L; human serum albumin 6g / L; oxidative auxiliary enzyme I 7mmol / L; reduced auxiliary enzyme I 14g / L; L.

[0024] And, wherein, the preservative is sodium nitrite.

[0025] Using the total bile acid detection reagent of the present invention to carry out test experiments on patients, it is found that compared with the existing test reagents, the test speed is faster, the accuracy is higher, the display is clearer, and it is easy to store and has a long shelf life; and, Strong anti-interference ability and more stable.

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Abstract

The invention relates to a total bile acid detection reagent. The detection reagent is composed of the following components: 6-8wt% of a preservative, 30-40mmol/L of sodium pyruvate, 30-40g/L of glycinate buffer solution, 8-10g/L of dextran sulfate, 2-3g/L of 2-hydroxy pyridine-N-oxide, 5-8g/L of a stabilizer, 30-40mmol/L of phosphatic buffer solution, 6000-7000u/L of diaphorase, 3-4g/L of sodium azide, 4-5mmol/L of a chemical oxidant, 2-3wt% of a free-drying protective agent, 30-40mmol/L of NaCl, 6-8KU/L of 3alpha-hydrocarbon sterol dehydrogenase, 3-10g/L of human serum albumin, 6-8mmol/L of an oxidizing auxiliary enzyme I, 12-15g/L of a reduced coenzyme I and 2-3g/L of nitrotetrazolium blue. The detection reagent provided by the invention is high in determination precision, strong in anti-interference capability, good in stability, long in retention period and easy to store and transport.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a detection reagent, in particular to a total bile acid detection reagent. Background technique [0002] The positive rate of serum total bile acid for liver cirrhosis and liver cancer is as high as 95%. The popularization of total bile acid detection has provided a more effective basis for the early diagnosis of various acute and chronic hepatitis, liver cirrhosis, and even liver cancer patients. The clinical diagnosis is of great significance Big. [0003] However, the stability of most total bile acid reagents currently used clinically is relatively poor. The bottle is stable within two weeks (determined value continues to decline); and the negative interference of most brands of total bile acid reagent bilirubin is obvious, and the recovery rate of the reagent is low. The recovery rate of most reagents is about 80%, and some are even as low as 60%. %, so that negative valu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 徐淼
Owner 徐淼
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