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Total bilirubin detection reagent

A technology for detecting reagents and total bilirubin, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by making materials undergo chemical reactions, etc. Unfavorable detection application and other problems, to achieve the effect of convenient clinical application, convenient measurement, and good color rendering effect

Inactive Publication Date: 2017-06-27
徐淼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, all of the above bilirubin testing methods generally have shortcomings such as low measurement precision, weak anti-interference ability, and unsuitability for automatic rapid measurement, which is not conducive to the application of clinical routine detection.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The composition of the total bilirubin detection reagent of this embodiment is: preservative 6wt%; Tris-HCl buffer solution 160mmol / L; Sodium lauryl sulfate 6g / L; Surfactant 1mmol / L; Sodium metavanadate 3mmol Vitamin C oxidase 70KU / L; Triton x-100 6wt%; phosphate buffer 30mmol / L; preservative 1wt%; chemical oxidant 3mmol / L; lyoprotectant 2wt%; Sodium 1g / L; sulfuric acid 1ml / L; sodium persulfate 300g / l; ethanolamine 300mmol / l.

[0020] And, wherein, the preservative is potassium sorbate.

Embodiment 2

[0022] The composition of the total bilirubin detection reagent of this embodiment is: preservative 8wt%; Tris-HCl buffer solution 180mmol / L; Sodium lauryl sulfate 9g / L; Surfactant 2mmol / L; Sodium metavanadate 4mmol Vitamin C oxidase 90KU / L; Triton x-100 8wt%; phosphate buffer 40mmol / L; preservative 2wt%; chemical oxidant 3mmol / L; lyoprotectant 3wt%; Sodium 2g / L; sulfuric acid 2ml / L; sodium persulfate 400g / l; ethanolamine 400mmol / l.

[0023] And, wherein, the preservative is sodium benzoate.

Embodiment 3

[0025] The composition of this embodiment ID total bilirubin detection reagent is: preservative 7wt%; Tris-HCl buffer solution 170mmol / L; Sodium lauryl sulfate 8g / L; Surfactant 1.5mmol / L; Sodium metavanadate 3.5mmol / L; vitamin C oxidase 80KU / L; Triton x-100 7wt%; phosphate buffer 35mmol / L; preservative 1.5wt%; chemical oxidant 3mmol / L; L; sodium azide 1.5g / L; sulfuric acid 1.5ml / L; sodium persulfate 350g / l; ethanolamine 350mmol / l.

[0026] And, wherein, the preservative is sodium nitrite.

[0027] The total bilirubin detection reagent of the present invention is used to carry out test experiments on patients, and it is found that compared with the existing test reagents, the test speed is faster, the accuracy is higher, the display is clearer, and it is easy to store and has a long shelf life; and , Strong anti-interference ability and more stable.

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PUM

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Abstract

The invention relates to a total bilirubin detection reagent. The detection reagent is composed of the following components: 6-8wt% of a preservative, 160-180mmol / L of Tris-HCl buffer solution, 6-9g / L of sodium dodecyl sulfate, 1-2mmol / L of a surfactant, 3-4mmol / L of sodium metavanadate, 70-90KU / L of ascorbic acid oxidase, 6-8wt% of Triton x-100, 30-40mmol / L of phosphatic buffer solution, 1-2wt% of a preservative, 3mmol / L of a chemical oxidant, 2-3wt% of a freeze-drying protective agent, 50-60mmol / L of NaCl, 1-2g / L of sodium azide, 1-2ml / L of sulphuric acid, 300-400g / l of sodium persulfate and 300-400mmol / l of ethanolamine. The detection reagent provided by the invention is high in determination precision, strong in anti-interference capability and applicable to automatic rapid determination.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a detection reagent, in particular to a total bilirubin detection reagent. Background technique [0002] Elevated bilirubin is seen in acute jaundice hepatitis, acute yellow hepatic necrosis, chronic active hepatitis, liver cirrhosis, hemolytic jaundice, blood type incompatibility transfusion reaction, neonatal jaundice, cholelithiasis, liver cancer, pancreatic head cancer, etc. Low bilirubin is seen in iron-deficiency anemia, which is anemia caused by the lack of iron in the body, which affects the synthesis of hemoglobin. The symptoms of iron-deficiency anemia are yellowish or pale complexion, but whether iron-deficiency anemia requires further examination, red blood cell morphology, serum iron, serum ferritin examination. If people who are anorexic are deficient in zinc, it will also cause low total bilirubin. [0003] Direct bilirubin determination is one of the commonly u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 徐淼
Owner 徐淼
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