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A plant rich in isobiflorin and its new extraction method

A plant and extract technology, applied in the field of isobiflorin extraction, can solve the problems of cumbersome separation methods, high cost, and low efficiency

Active Publication Date: 2019-08-23
FUJIAN LICHUAN BIOLOGICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] It has been reported in the literature that this component exists in the Phytophthora chrysophylla ( Dryopteris crassirhizoma ), Amaryllidaceae Almighty Flower ( Pancratium biflorum ), myrtle plant clove ( Syzygium aromaticum ) and other plants, and the existing technology, isobiflorin obtained from cloves is the highest, but the yield is only 0.1%, which can be developed and utilized is low, and the separation method is relatively cumbersome. After extraction, it needs to go through extraction, gel chromatography and liquid chromatography Separating and finally obtaining monomeric compounds, if used in production, the cost is high and the efficiency is low

Method used

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  • A plant rich in isobiflorin and its new extraction method
  • A plant rich in isobiflorin and its new extraction method
  • A plant rich in isobiflorin and its new extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 1,000 g of the fruit peel powder of Coryda sinensis, add 10,000 mL of 75% ethanol solution, heat and boil for 2 hours, and filter to obtain the extract; add 8,000 mL of 75% ethanol solution to the filter residue, boil again for 2 hours, and filter to obtain the extract; The two extracts were combined, concentrated under reduced pressure to dryness to obtain 205 g of the extract; the extract was mixed with 205 g of 60-100 mesh silica gel, 800 g of 200-300 mesh silica gel was dry-packed, and dichloromethane-ethanol-water (5 :1:0.2) 5000 mL to remove impurities, dichloromethane-ethanol-water (2:1:0.5) 5000 mL to elute, collect the eluate, recover the solvent under reduced pressure, and obtain 18.3 g of extract. Add ethanol, boil until the extract is completely dissolved, let cool, and precipitate 11.2 g of white solid powder. The sample was dissolved in dimethyl sulfoxide (DMSO) and separated by high performance liquid chromatography under the conditions of detection ...

Embodiment 2

[0025] Take 1000 g of the seed powder of C. rotundum, and operate according to the method of Example 1, and finally obtain 2.3 g of the refined product of isobiflorin, with a yield of 0.23% and a purity of 97.4%.

Embodiment 3

[0027] Take 1000 g of the powder of Tsubaki sylvatica, operate according to the method of Example 1, and finally obtain 2.7 g of the refined product of isobiflorin, with a yield of 0.27% and a purity of 96.2%.

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Abstract

The invention provides an isobiflorin-rich plant which is a Euscaphis konishii plant. The isobiflorin content of all organs of the plant is 0.14%-0.45%, the isobiflorin content of peel is the highest, and a new source is provided for an isobiflorin compound. The invention further provides a matching production method for extracting isobiflorin from the plant. According to the method for extracting the isobiflorin from Euscaphis konishii, the dried Euscaphis konishii plant serves as a raw material and is subjected to crushing, ethanol solution extraction, extracting solution concentration extract preparation, silica gel column chromatography elution and eluant concentration recrystallization to obtain white powdery crystals, the powdery crystals are subjected to high-performance liquid chromatographic separation to obtain an isobiflorin monomeric compound, gel chromatography separation and liquid chromatography separation are omitted, and the method is simple in process, low in cost and safe.

Description

technical field [0001] The patent of the present invention relates to a plant rich in isobiflorin and a new method for extracting isobiflorin from the plant. Background technique [0002] isobiflorin, Chinese name: 5,7-dihydroxy-2-methyl-chromone-8- C - β -D-glucoside, white powder crystal, molecular formula is C 16 h 18 o 9 , molecular weight 354; the compound is a chromone compound. According to literature reports, the compound has good anti-inflammatory and anti-oxidation effects. [0003] It has been reported in the literature that this component exists in the Phytophthora chrysophylla ( Dryopteris crassirhizoma ), Amaryllidaceae Almighty Flower ( Pancratium biflorum ), myrtle plant clove ( Syzygium aromaticum ) and other plants, and the existing technology, isobiflorin obtained from cloves is the highest, but the yield is only 0.1%, which can be developed and utilized is low, and the separation method is relatively cumbersome. After extraction, it needs to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D407/04
Inventor 倪林梁文贤邹双全徐会有黄维邹小兴孙维红马岱袁雪燕
Owner FUJIAN LICHUAN BIOLOGICAL CO LTD