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Sodium monosialotetrahexosylganglioside preparation method

A technology of sodium ganglioside and monosialic acid, which is applied to the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of many adverse reactions and insufficient extraction techniques, and achieve good results and high purity high effect

Inactive Publication Date: 2017-06-30
四川奇格曼药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its origin from pig brain, a biological raw material, monosialotetrahexosylganglioside sodium has more adverse reactions, especially allergic reactions related to the formation of impurities, pollutants, additives, viruses or special metabolites in the drug , and the current extraction technology is not fine enough, the extracted monosialotetrahexosyl ganglioside sodium will also contain trace amounts of harmful substances and impurities

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of monosialotetrahexosylganglioside sodium, comprising the following steps:

[0025] (1) Stirring with fresh pig brain as a raw material to obtain a concentrated solution of fresh pig brain;

[0026] (2) Resin chromatography is performed on the prepared concentrated solution, and suction filtration is dried to obtain concentrated powder:

[0027] (3) Silica gel crude purification step: Dissolve the concentrated powder in a mobile phase prepared from methanol, trichloride and pure water, then perform silica gel chromatography, and precipitate and dry to obtain a crude concentrated powder;

[0028] (4) Silica gel purification step: Dissolve the crude concentrate powder in a chromatography buffer prepared from methanol, trichloride and pure water, then perform silica gel chromatography, and precipitate and dry to obtain a fine concentrate powder.

[0029] Among them, in step (3), the dissolving sample (the concentrated powder is dissolved in the mobil...

Embodiment 2

[0037] In this embodiment, on the basis of Embodiment 1, the step (1) includes: thawing the pig brain, sorting impurities, stirring, filter-pressing, layering, and decomposing to obtain a fresh pig brain concentrate. Take out 200kg / pot pig brains from the cold storage; after the pig brains are thawed, remove the blood water in the thawing tank, pick out the sundries, scoop the thawed pig brains in the thawing tank into the hopper of the meat grinder, and collect the brain pulp in the collection tank; clear the field And clean all equipment and tools.

[0038] The other parts of this embodiment are the same as those in Embodiment 1, and are not repeated here.

Embodiment 3

[0040] In this embodiment, on the basis of the above-mentioned embodiment, the step (2) includes: preparation of a new resin and enhanced regeneration of the resin, adsorption and cleaning of the resin and methanol elution, concentration of the resin and fresh pig brain concentrate, acetone precipitation, Suction filtration and drying to obtain concentrated powder.

[0041] The other parts of this embodiment are the same as the above-mentioned embodiments, and are not repeated here.

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Abstract

The invention discloses a sodium monosialotetrahexosylganglioside preparation method, which comprises: stirring a raw material such as fresh pig brain to obtain a fresh pig brain concentrated liquid; carrying out resin chromatography on the prepared concentrated liquid, and carrying out suction filtration drying to obtain concentrated powder; dissolving the concentrated powder in a mobile phase, carrying out silica gel chromatography, and carrying out precipitation and drying to obtain crude product concentrated powder; and dissolving the crude product concentrated powder in a chromatographic buffer liquid, carrying out silica gel chromatography, and carrying out precipitation and drying to obtain fine product concentrated powder. According to the present invention, the purification step is divided into the crude purification step and the fine purification step, such that the purity of the prepared sodium monosialotetrahexosylganglioside is high compared to the method in the prior art; and the resin used in the resin chromatography step is subjected to the specific acid and alkali treatment and the cleaning process, and the resin chromatography column is subjected to the strengthening regeneration treatment.

Description

technical field [0001] The invention relates to the field of pharmaceutical preparation methods, in particular to a preparation method of monosialotetrahexosylganglioside sodium. Background technique [0002] Monosialotetrahexosylganglioside sodium is a substance that is extracted from pig brain and has an effect on nerve cell function damage. In the prior art, the extraction of monosialotetrahexosylganglioside sodium is based on improving product purity, yield, reducing product cost, and reducing the usage of toxic organic solvents (especially chloroform) A lot of research has been carried out. , and a variety of products such as Monosialotetrahexosylganglioside Sodium Injection, Monosialotetrahexosylganglioside Sodium for Injection and so on have been sold and used in the market. However, due to its origin from the biological raw material pig brain, monosialotetrahexosylganglioside sodium has many adverse reactions, especially allergic reactions related to the formation o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/10C07H1/08
CPCC07H1/08C07H15/10
Inventor 张宪姚静李豫俭
Owner 四川奇格曼药业有限公司
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