Bacterial colony total number rapid detection card and application method thereof

Abstract

The invention relates to the field of food hygiene and safety, particularly to a bacterial colony total number rapid detection card and an application method thereof. The bacterial colony total number rapid detection card is compose of a lower layer and an upper layer, wherein the lower layer is composed of a first transparent resin layer, a first transparent double-faced adhesive layer, a first gel layer and a ventilation layer which are sequentially arranged from bottom to top; the upper layer is composed of a second gel layer, a second transparent double-faced adhesive layer and a second transparent resin layer; the upper layer and the lower layer are in hinge connection; the ventilation layer is provided with through holes for placing sample solutions; the first gel layer is uniformly mixed with cultivation matrix powder. The application method comprises taking hollow-out blow molding paper as the ventilation layer and is cultivated in an incubator of 35-37 DEG C for 24-28 hours, counting the number of bacterial colonies, and performing conversion to obtain the total number of bacterial colonies in the sample. The structure and the application method of the bacterial colony total number rapid detection card have the advantages that dry powder are uniformly adhered onto transparent double-faced adhesives; the ventilation layer is environmentally friendly in materials and facilitates through hole forming; the transparent resin layers are provided with squares through printing for facilitating number counting. During application, the bacterial colony total number rapid detection card and the application method thereof are rapid in operation, high in sensitivity, accurate in counting and low in cost.

Description

technical field [0001] The invention relates to the technical field of food hygiene and safety, in particular to a rapid detection card for the total number of bacterial colonies and a use method thereof. Background technique [0002] Food is the material basis for human survival and development, and food safety is related to people's health and national economy and people's livelihood, and has been paid more and more attention. The total number of bacterial colonies is the most basic and common microbial index test item in food hygiene inspection. [0003] The International Microbiological Specification Committee defines the total number of colonies as the unit weight (g), volume (mL) or surface area (cm 2 ) contains the total number of colonies that can be grown on a solid medium under certain conditions. The total number of bacterial colonies is an important indicator to determine the degree of bacterial contamination of food, and can reflect whether the food processing ...

AI Technical Summary

Problems solved by technology

[0004] At present, the detection of the total number of colonies in food is generally carried out in accordance with the standard of GB / T4789.2-2010 "Food Hygiene Microbiological Examination Determination of the Total Number of Colonies" by the pouring plate counting method, which has high accuracy and sensitivity, but involves There are many experiments, cumbersome operations, long time-consuming, heavy preparation and finishing work, and a large number of people involved

Images