French marigold general flavone extract, and preparation method and application thereof

A technology of peacock grass and total flavonoids, which is applied in the direction of medical formula, drug combination, plant raw materials, etc., can solve the problems of weakened curative effect, increased toxicity, and uncontrollable process

Inactive Publication Date: 2017-07-04
蔡德成 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, western medicine is the main treatment method, but it only improves the symptoms, but cannot control its progress, and the curative effect is weakened and the toxicity increases after long-term application.

Method used

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  • French marigold general flavone extract, and preparation method and application thereof
  • French marigold general flavone extract, and preparation method and application thereof
  • French marigold general flavone extract, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 prepares the total flavonoids of Malachite

[0018] Preparation of total flavonoids of malachite grass by solvent extraction and resin adsorption:

[0019] 1 kg of malachite grass powder with 10 meshes and 80% ethanol by volume were soaked and extracted twice, each time the amount of solvent was 10 times of the mass of the medicinal material, and soaked at 40°C for 12 hours each time. Combine the extracts, recover the ethanol solution, concentrate to about 1 times the mass of the medicinal material (about 1 liter), and extract 3 times with 500 ml of petroleum ether each time to obtain a degreasing solution. The degreasing solution is eluted with water (2500 milliliters) and 60% ethanol aqueous solution (2000 milliliters) successively through the pretreated HPD100 macroporous adsorption resin column (about 500 milliliters), and the 60% ethanol eluent is recovered under reduced pressure Ethanol, dried under reduced pressure to obtain 105g of total flavonoids...

experiment example 2

[0020] Experimental Example 2 Research on the Anti-Parkinson Effect of Malachite Total Flavonoids

[0021] 1. Experimental materials

[0022] C57BL / 6 mice were purchased from the Animal Experiment Center of Dalian Medical University, and the total flavonoids of malachite grass used were self-made by the above-mentioned process.

[0023] 2. Grouping of animals

[0024] 50 C57BL / 6 mice were randomly divided into 5 groups: blank group, model group, positive drug group (Dopaserazide Tablets (Madopar), Shanghai Roche Pharmaceutical Co., Ltd., batch number: SH1346) and malachite total flavonoids High and low dose groups, 10 rats in each group.

[0025] 3. Modeling and administration methods

[0026] The model group and the high- and low-dose groups were subcutaneously injected with MPTP (purchased from Sigma, batch number: 1001054803) 25 mg / kg, once a day, for 3 consecutive days. On the fourth day, the extract of total flavonoids of Malacock grass was given by intragastric admin...

Embodiment 3

[0039] Example 3 Anti-Parkinson's Serum Pharmacology Study of Malachite Total Flavonoids

[0040] 1. Experimental materials

[0041] Methods SD rats, half male and half male, weighing 200±20 g, were purchased from Dalian Medical University. The total flavonoids of malachite used are self-made by adopting the above-mentioned process. Weigh an appropriate amount of total flavonoids from Malachite, and prepare a 5 mg / mL solution for later use.

[0042] 2. Method

[0043] 2.1 Preparation of drug serum

[0044] The rats were adaptively fed for 3 days and had free access to food and water. They were randomly divided into a blank group and a high and low dose group of malachite total flavonoids. The high-dose and low-dose groups were given 60 mg / kg and 30 mg / kg, respectively, twice a day by intragastric administration for 7 consecutive days; the blank group was given the same amount of normal saline. 0.5 hours after the last administration, the eyeballs were removed to take blo...

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Abstract

The invention discloses an application of French marigold general flavone extract in the preparation of anti-Parkinson's drugs. A preparation method of the French marigold general flavone extract comprises the following steps: 1, extracting French marigold by an ethanol-water solution at least one time to obtain an extract liquid, and concentrating the extract liquid to obtain a concentrate; 2, degreasing the concentrate by a degreasing solvent to obtain a degreased concentrate; and 3, separating and purifying the degreased concentrate to obtain the French marigold general flavone extract. The weight content of general flavones in the French marigold general flavone extract, detected through colourimetry, is higher than 30%. Pharmacological test proves that the French marigold general flavone extract has high anti-Parkinson's activity, and can be applied to preparations of the anti-Parkinson's drugs individually or after being combined with other drugs. The purity of the general flavones in the French marigold general flavone extract prepared through the method is high, and the preparation method is simple, so the extract is suitable for industrial production; and the French marigold general flavone extract can be used for preparing the anti-Parkinson's drugs.

Description

technical field [0001] The invention relates to a herbal medicine and its application, in particular to a medicine extract with malachite total flavonoids as an active ingredient and its use in preparing anti-Parkinson medicine. Background technique [0002] Peacock grass (Tagetes patula L.), also known as red yellow grass, rattan chrysanthemum, French marigold, and small marigold, is a plant of the genus Marigold in the Asteraceae family. It is an annual herb with a flowering period from July to September. It is cultivated all over our country and is a common ornamental flower. Peacock grass has medicinal and health care functions. The flowers and leaves can be used as medicine. Can treat whooping cough, tracheitis, cold and so on. [0003] Since the 1980s, people began to study the chemical constituents of Malachite, and isolated flavonoids such as patuletin, quercetagetin, and quercetagitrin ( State Administration of Traditional Chinese Medicine "Chinese Materia Medica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P25/16C07G99/00
CPCA61K36/28A61K2236/333A61K2236/39A61K2236/53A61K2236/51A61K2236/55C07G99/00
Inventor 蔡德成窦德强
Owner 蔡德成
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