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Analytical method for distinguishing genuine and holiday-sunshine green tea using metabolomics technology based on uhplc tandem high-resolution mass spectrometry

A high-resolution mass spectrometry and analysis method technology, applied in the field of tea quality inspection, can solve problems such as incompatibility, qualitative and quantitative determination of chemical substances, etc., and achieve the effects of high confidence, convenient operation and high resolution

Active Publication Date: 2019-11-01
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Application Information

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Problems solved by technology

There are hundreds of chemical components in tea such as water, alkaloids, enzymes, tea polyphenols, vitamins, and amino acids. These chemical components are also interrelated, and together they determine the quality of tea. The method of tea quality appraisal cannot qualitatively and quantitatively measure the chemical substances in tea. It is too subjective and gradually does not adapt to the current development of the tea industry economy. It is difficult to be convincing.

Method used

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  • Analytical method for distinguishing genuine and holiday-sunshine green tea using metabolomics technology based on uhplc tandem high-resolution mass spectrometry
  • Analytical method for distinguishing genuine and holiday-sunshine green tea using metabolomics technology based on uhplc tandem high-resolution mass spectrometry
  • Analytical method for distinguishing genuine and holiday-sunshine green tea using metabolomics technology based on uhplc tandem high-resolution mass spectrometry

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Embodiment 1

[0071] An analysis method for distinguishing Rizhao green tea and low-priced green tea based on UHPLC tandem quadrupole-orbitrap high-resolution mass spectrometry using metabolomics technology, comprising the following steps:

[0072] (1) Sample pretreatment

[0073] Crush the tea sample, weigh 0.1g of the sample into a 50mL centrifuge tube, add 35mL of extractant (75% aqueous methanol (v / v)), vortex and mix to dissolve, sonicate for 20min, and centrifuge at 1000rpm for 5 minutes, then extract the solution Mix well, filter with a 0.22-micron membrane filter, and put it on the machine with an injection volume of 2 microliters.

[0074] (2) UHPLC was used in series with Thermo Fisher Q Exactive Mass Spectrometer to realize the separation and determination of chemical components in the sample.

[0075] 1) Liquid chromatography parameters

[0076] Chromatographic column: Water BEH C18 (1.7μm 2.1x50mm) chromatographic column.

[0077] Liquid phase: Phase A, 0.1% formic acid in wat...

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Abstract

The invention discloses an analysis method for distinguishing true Rizhao green tea from fake Rizhao green tea on the basis of a UHPLC tandem high-resolution mass spectrum and by using the metabonomic technology. A Rizhao green tea sample and a to-be-detected green tea sample are subjected to pretreatment by means of organic solvents respectively, the ultra-high-efficiency liquid chromatography-quadrupole rod-rail trap high-resolution mass spectrum method is used for achieving separation and measurement of chemical components in the sample obtained after pretreatment, then UHPLC-MS original data of the Rizhao green tea sample and the to-be-detected green tea sample is preprocessed, and finally whether the to-be-detected green tea sample is the Rizhao green tea or not is distinguished by means of a main component analysis model by using the multivariate statistical analysis method. Analysis results are displayed in a PCA score plot mode. Technicians in the field can visually judge the true or false conditions of the Rizhao green tea, related verification and analysis do not need to be conducted to obtain true or false conclusions, and detection results are accurate and reliable.

Description

technical field [0001] The invention belongs to the technical field of tea industry quality detection, and in particular relates to an analysis method for distinguishing true and false green tea based on UHPLC tandem high-resolution mass spectrometry using metabolomics technology. Background technique [0002] Metabolomics is a qualitative and quantitative analysis of all small molecular metabolites in a biological system at a given time and condition, thereby quantitatively describing the whole body of biological endogenous metabolites and their response to internal and external factors. The object of metabolomics research is small molecular metabolites with a relative molecular mass of less than 1000, which can systematically and meticulously understand and understand the activity rules and metabolic processes of living organisms. [0003] The main research idea of ​​metabolomics is a "global perspective", focusing on the overall situation of biological metabolites, which ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/06G01N30/14G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/14G01N30/34G01N30/72G01N30/8679G01N2030/146
Inventor 祝建华耿越王骏王凯利宿书芳江瑶
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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