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Fermentation culture medium and fermentation method for producing levodopa

The technology of fermentation medium and levodopa, which is applied in the field of microbial fermentation, can solve the problems of poor fermentation effect, high cost of fermentation raw materials, long fermentation period and the like, and achieves the effects of low cost, simple preparation and increased biomass.

Inactive Publication Date: 2017-07-14
SHANDONG LUKANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the present invention provides a fermentation medium and a fermentation method for producing levodopa, which solve the disadvantages of high cost of fermentation raw materials, long fermentation period and poor fermentation effect in the prior art

Method used

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  • Fermentation culture medium and fermentation method for producing levodopa

Examples

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Effect test

Embodiment 1

[0044] Prepare the fermentation medium according to the following formula:

[0045] Fermentation medium formula (g / L): yeast extract 10g, peptone 10g, disodium hydrogen phosphate dodecahydrate 15g, sodium sulfate 7g, sodium chloride 1.0g, ammonium chloride 4g, dipotassium hydrogen phosphate trihydrate 10g, lemon acid 0.5g, magnesium sulfate heptahydrate 0.5g, glycerin 5ml.

Embodiment 2

[0047] Prepare the fermentation medium according to the following formula:

[0048] Fermentation medium formula (g / L): yeast extract 7g, peptone 9g, disodium hydrogen phosphate dodecahydrate 12g, sodium sulfate 7g, sodium chloride 0.5g, ammonium chloride 3g, dipotassium hydrogen phosphate trihydrate 9g, lemon acid 0.4g, magnesium sulfate heptahydrate 0.3g, glycerin 3ml.

Embodiment 3

[0050] Prepare the fermentation medium according to the following formula:

[0051] Fermentation medium formula (g / L): Yeast extract 14g, peptone 13g, disodium hydrogen phosphate dodecahydrate 18g, sodium sulfate 10g, sodium chloride 1.5g, ammonium chloride 6g, dipotassium hydrogen phosphate trihydrate 46g, lemon acid 1g, magnesium sulfate heptahydrate 1g, glycerin 10ml.

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Abstract

The invention relates to the technical field of microbial fermentation, and discloses a fermentation culture medium for producing levodopa. Every liter of the fermentation culture medium is prepared from the following components: 5-14g of yeast extract, 7-13g of peptone, 10-18g of sodium phosphate dibasic dodecahydrate, 5-10g of sodium sulfate, 0.1-0.5 g of sodium chloride, 1-6g of ammonium chloride, 7-16g of dipotassium hydrogen phosphate trihydrate, 0.1-1g of citric acid, 0.1-1g of magnesium sulfate heptahydrate and 1-10ml of glycerol. The invention also comprises a fermentation method for producing the levodopa by using the fermentation culture medium. When being used for fermenting a seed solution, the fermentation culture medium can improve the thallus vitality and enable cold-induced thallus to grow rapidly, thus can shorten the fermentation period and increasing biomass of the thallus. The fermentation culture medium is simple in formulation and lower in cost.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a fermentation medium and a fermentation method for producing levodopa. Background technique [0002] Levodopa, also known as 3,4-dihydroxyphenylalanine, is a white crystalline powder, odorless and tasteless, insoluble in ethanol, chloroform and acetone, soluble in dilute acid, as an important biologically active substance , Levodopa is the precursor of the neurotransmitter dopamine, which can pass through the blood-brain barrier, enter the cerebral circulation, and reach the central nervous system. Treat Parkinson's disease. Its structural formula is as follows: [0003] [0004] Levodopa is the first choice and the most effective drug for the treatment of senile Parkinson's disease. In addition, Levodopa also has the effect of treating amblyopia and heart failure. Although there will be some side effects after long-term use, more than 40 years old Years hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/22C12N1/21C12R1/19
CPCC12N1/20C12P13/225
Inventor 金伟郑长春王博慕东张兴灿卢春玲陈君张勇
Owner SHANDONG LUKANG PHARMA
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