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Drug-loading ganglioside micelle as well as preparation method and application thereof

A ganglioside and micelle technology, which is applied in the directions of pharmaceutical formulations, nervous system diseases, drug combinations, etc., to achieve the effect of a simple method

Inactive Publication Date: 2017-07-25
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no relevant literature on drug-loaded GM1 micelles for intracranial drug delivery through the blood-brain barrier

Method used

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  • Drug-loading ganglioside micelle as well as preparation method and application thereof
  • Drug-loading ganglioside micelle as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Preparation of DOX-GM1 micelles

[0049] Preparation of micelles: DOX-GM1 micelles were prepared by dialysis. Accurately weigh the GM1 powder in a beaker, add an appropriate amount of tri-distilled water and stir to fully dissolve it. Place the GM1 solution in a refrigerator at 4°C for about 20 hours, centrifuge for 30 minutes, filter the solution, and place it in a refrigerator at 4°C for about 20 hours. Standby; Dissolve the weighed DOX powder in an appropriate amount of organic solvent to fully dissolve it. Add the DOX solution to the GM1 solution slowly and with stirring; incubate the mixed DOX-GM1 solution in a refrigerator at 4°C, about 20 After hours, the solution was transferred to a dialysis bag for 24 hours for dialysis. Finally, the DOX-GM1 solution was placed in a refrigerator at 4°C for 24 hours before being used for experiments.

Embodiment 2

[0050] Example 2 Preparation of DOX-LIGA micelles

[0051] Accurately weigh the LIGA powder in a beaker, add an appropriate amount of tri-distilled water and stir to fully dissolve it. Place the LIGA solution in a refrigerator at 4°C for about 20 hours, centrifuge for 30 minutes, then filter the solution, and place in a refrigerator at 4°C for about 20 hours. Standby; Dissolve the weighed DOX powder in an appropriate amount of organic solvent to fully dissolve it. Add the DOX solution to the LIGA solution slowly and with stirring; incubate the mixed DOX / LIGA solution in a refrigerator at 4°C, about 20 After hours, the solution was transferred to a dialysis bag for 24 hours for dialysis. Finally, the DOX-LIGA solution was placed in a refrigerator at 4°C for 24 hours before being used for experiments.

Embodiment 3

[0052] Example 3 Preparation of CUR-GM1 micelles

[0053] Accurately weigh the GM1 powder in a beaker, add an appropriate amount of tri-distilled water and stir to fully dissolve it. Place the GM1 solution in a refrigerator at 4°C for about 20 hours, centrifuge for 30 minutes, filter the solution, and place it in a refrigerator at 4°C for about 20 hours. Standby; Dissolve the weighed curcumin powder in an appropriate amount of organic solvent to fully dissolve it, add the curcumin solution to the GM1 solution slowly and with stirring; place the mixed CUR-GM1 solution in the refrigerator at 4°C and incubate, After about 20 hours, the solution was transferred to a dialysis bag for 24 hours for dialysis. Finally, the CUR-GM1 solution was placed in a refrigerator at 4°C for 24 hours before it could be used in the experiment.

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PUM

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Abstract

The invention belongs to the technical field of bio-medicine, and in particular relates to a drug-loading ganglioside micelle as well as a preparation method and an application thereof. According to the invention, the application of a GM1 (ganglioside micelle 1) and derivatives thereof as a nano drug-delivery system carrier material is provided; blood brain barrier is a key barrier in intracranial drug delivery; and the nano drug-delivery system, which offers a powerful tool to noninvasive intracranial combined administration, has a great clinical significance. The drug-loading ganglioside micelle, which is prepared by taking the GM1 and the derivatives thereof as a nano drug-delivery system carrier, can implement nerve injury repair and intracranial combined administration in a mode of penetrating the blood brain barrier without the introduction of other carrier materials, long-circulation modifying materials or nerve repair drugs; and it is expected that the drug-loading ganglioside micelle provided by the invention can offer a novel view angle and method for the improvement of clinical treatment.

Description

Technical field [0001] The invention belongs to the technical field of biomedical materials, and specifically relates to a drug-loaded ganglioside micelle and a preparation method and application thereof. Background technique [0002] In pharmacotherapy, the existence of the blood-brain barrier prevents almost all macromolecular drugs and many small molecule drugs from entering the brain and central nervous system, and whether the drugs can cross the BBB and achieve effective therapeutic concentrations. Treatment of glioma It is very important. After traditional chemotherapy is administered, due to the body's own physiological response, it cannot directly and effectively act on the lesion. The blood-brain barrier is a key obstacle for intracranial drug delivery to treat brain diseases. [0003] The so-called blood-brain barrier is a selective permeable "Neurovascular Unit" (NVU) composed of specific physical structures, various enzymes and transport proteins. Its specific barrier ...

Claims

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Application Information

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IPC IPC(8): A61K9/107A61K47/26A61K31/704A61K31/12A61P25/00A61P35/00
CPCA61K9/1075A61K31/12A61K31/704A61K47/26A61K9/0019A61K31/65A61P9/12A61P25/00A61P25/08A61P25/24A61P25/28A61P35/00
Inventor 王亚洲邹丹尹铁英王贵学
Owner CHONGQING UNIV
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