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Cucumber breeding method based on induced polyploid and identification method of polyploid plants

An identification method and polyploidy technology, applied in the directions of botanical equipment and methods, plant genetic improvement, application, etc., to achieve the effect of improving economic benefits

Inactive Publication Date: 2017-08-01
HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

Although plant polyploid mutagenesis technology has been widely used, the existing cucumber germplasm resources cannot meet people's needs due to the strong market demand for cucumbers.

Method used

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  • Cucumber breeding method based on induced polyploid and identification method of polyploid plants
  • Cucumber breeding method based on induced polyploid and identification method of polyploid plants
  • Cucumber breeding method based on induced polyploid and identification method of polyploid plants

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Embodiment 1

[0060] 1 Materials and methods

[0061] 1.1 Test material

[0062] The seeds of three types of cucumbers (South China type 'TZT', North China type 'D19', and European greenhouse type 'NS3') provided by the Cucumber Research Group of Hebei Science and Technology Normal University were used as experimental materials.

[0063] 1.2 Polyploid induction

[0064] After the three types of cucumber seeds germinated at 30°C for 36 hours, they were sown in 50-hole trays and placed in a solar greenhouse for seedling cultivation. When the two cotyledons are fully unfolded, put the cotton ball on the growing point, use a rubber dropper to absorb the colchicine solution, drop it on the growing point cotton ball, and completely soak the cotton ball. Three colchicine concentrations (0.1wt%, 0.2wt%, 0.3wt%) were used to treat once respectively at 7:00-8:00 in the morning and 5:00-6:00 in the afternoon, and the treatment times were 3 times, 5 times and 7 times, a total of 3 × 3 treatments, 10...

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Abstract

The invention relates to the technical field of plant mutation breeding, and concretely relates to a cucumber breeding method based on an induced polyploid and an identification method of polyploid plants. The cucumber breeding method comprises the steps of breeding germinated cucumber seeds into seedlings, carrying out induction treatment on growing points of the seedlings by using 0.25 to 0.32 percent of colchicine solution, wherein the treatment is carried out for 4 to 6 times; then carrying out plant morphology identification, stoma identification, flow cytometry identification and cytology identification on a diploid and variant plants. According to the method, a cucumber polyploidy new strain suitable for production and application can be effectively bred, so that the economic benefit is improved.

Description

technical field [0001] The invention relates to the technical field of plant mutation breeding, in particular to a method for breeding cucumbers based on induced polyploidy and an identification method for polyploid plants. Background technique [0002] Because of its "hugeness", polyploidy can improve crop yield and quality, enhance disease resistance and stress resistance, and is widely used in production. Polyploid breeding has been widely used in food crops (Luo Xiaojin and He Haohua, 2011), fruit and vegetable crops (Chen Jinfeng et al., 2004; Ma Yue et al., 2015, Amaral et al., 2015), ornamental plants (Li Han et al., 2009, Li Doudou et al. , 2013, Chen et al., 2016) and medicinal plants (Wang Chaoliang et al., 2007, Wang Yuehua et al., 2010) breeding work, used to improve the agronomic traits of diploid parents. Using polyploids as intermediate materials for breeding to obtain triploids (Diao Weiping, 2009; Diao et al., 2009), and additional line materials have impor...

Claims

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Application Information

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IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 郑金双孙成振宋晓飞李晓丽闫立英
Owner HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
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