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Quinoa Dimorphic Indel Molecular Marker and Its Development Method and Application

A molecular marker, quinoa technology, applied in biochemical equipment and methods, recombinant DNA technology, DNA / RNA fragments, etc., can solve problems such as confusion, lower genotyping accuracy, inconvenient use, etc., to reduce reading bands Error rate, ease of use, and abundant effects

Active Publication Date: 2020-09-29
JIANGSU ACAD OF AGRI SCI
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  • Summary
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  • Description
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Problems solved by technology

In particular, some SSR molecular markers can detect as many as dozens of alleles, which will cause confusion during the genotyping process, resulting in reduced genotyping accuracy and inconvenient use.

Method used

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  • Quinoa Dimorphic Indel Molecular Marker and Its Development Method and Application
  • Quinoa Dimorphic Indel Molecular Marker and Its Development Method and Application
  • Quinoa Dimorphic Indel Molecular Marker and Its Development Method and Application

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Embodiment Construction

[0022] The experimental methods in the following examples are conventional methods unless otherwise specified, and the involved experimental reagents and materials are conventional biochemical reagents and materials unless otherwise specified.

[0023] 1. Acquisition of quinoa genome sequence

[0024] The aerial parts of 11 quinoa germplasms at seedling stage were collected for DNA extraction. Genome sequencing libraries were constructed according to the operating instructions provided by Illumina. Genome Paired-end sequencing was performed using the Illumina HiSeq2500 sequencing platform of Berry and Kang Company, as shown in Table 1.

[0025] Table 1 The quinoa genome sequencing germplasm and its sequencing information

[0026]

[0027]

[0028] 2. Prediction and verification of quinoa dimorphic InDel molecular markers

[0029] The quinoa genome sequencing data (FASTQ format) was imported into mInDel software, and after quality control of the sequencing data, the hi...

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Abstract

The invention belongs to the technical field of molecular markers and particularly relates to a quinoa dimorphism InDel molecular marker as well as a development method and application thereof. The molecular marker types allotrtraploid quinoa genes through forward primers and reverse primers corresponding to 38 sites, so that the accuracy of quinoa gene typing can be greatly improved, and the tape reading error rate of the high polymorphic molecular marker in quinoa gene typing is lowered.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and in particular relates to a quinoa dimorphic InDel molecular marker and a development method and application thereof. Background technique [0002] InDel (Insertion / Deletion), also known as insertion-deletion, usually refers to the insertion or deletion of a certain number of nucleotides in another genome sequence compared to one genome sequence. The primers designed to amplify these sites according to the genomic insertion and deletion sites are InDel molecular markers. The marker can display the length difference of the amplified sequence, has the characteristics of high stability and co-dominance, and can be used for genetic map construction, genetic diversity analysis, population structure evaluation, and core germplasm screening. Since there are abundant InDel variations in the genome, and InDel variations can occur in genes or gene regulatory regions, InDel variations have the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张体付赵涵
Owner JIANGSU ACAD OF AGRI SCI
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