Supercharge Your Innovation With Domain-Expert AI Agents!

Quinoa Dimorphic Indel Molecular Marker and Its Development Method and Application

A molecular marker, quinoa technology, applied in biochemical equipment and methods, recombinant DNA technology, DNA / RNA fragments, etc., can solve problems such as confusion, lower genotyping accuracy, inconvenient use, etc., to reduce reading bands Error rate, ease of use, and abundant effects

Active Publication Date: 2020-09-29
JIANGSU ACAD OF AGRI SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, some SSR molecular markers can detect as many as dozens of alleles, which will cause confusion during the genotyping process, resulting in reduced genotyping accuracy and inconvenient use.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quinoa Dimorphic Indel Molecular Marker and Its Development Method and Application
  • Quinoa Dimorphic Indel Molecular Marker and Its Development Method and Application
  • Quinoa Dimorphic Indel Molecular Marker and Its Development Method and Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The experimental methods in the following examples are conventional methods unless otherwise specified, and the involved experimental reagents and materials are conventional biochemical reagents and materials unless otherwise specified.

[0023] 1. Acquisition of quinoa genome sequence

[0024] The aerial parts of 11 quinoa germplasms at seedling stage were collected for DNA extraction. Genome sequencing libraries were constructed according to the operating instructions provided by Illumina. Genome Paired-end sequencing was performed using the Illumina HiSeq2500 sequencing platform of Berry and Kang Company, as shown in Table 1.

[0025] Table 1 The quinoa genome sequencing germplasm and its sequencing information

[0026]

[0027]

[0028] 2. Prediction and verification of quinoa dimorphic InDel molecular markers

[0029] The quinoa genome sequencing data (FASTQ format) was imported into mInDel software, and after quality control of the sequencing data, the hi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of molecular markers and particularly relates to a quinoa dimorphism InDel molecular marker as well as a development method and application thereof. The molecular marker types allotrtraploid quinoa genes through forward primers and reverse primers corresponding to 38 sites, so that the accuracy of quinoa gene typing can be greatly improved, and the tape reading error rate of the high polymorphic molecular marker in quinoa gene typing is lowered.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and in particular relates to a quinoa dimorphic InDel molecular marker and a development method and application thereof. Background technique [0002] InDel (Insertion / Deletion), also known as insertion-deletion, usually refers to the insertion or deletion of a certain number of nucleotides in another genome sequence compared to one genome sequence. The primers designed to amplify these sites according to the genomic insertion and deletion sites are InDel molecular markers. The marker can display the length difference of the amplified sequence, has the characteristics of high stability and co-dominance, and can be used for genetic map construction, genetic diversity analysis, population structure evaluation, and core germplasm screening. Since there are abundant InDel variations in the genome, and InDel variations can occur in genes or gene regulatory regions, InDel variations have the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张体付赵涵
Owner JIANGSU ACAD OF AGRI SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com