Buffer solution for preparing competent cells and method for preparing competent cells
A technology of competent cells and buffers, which is applied in the field of preparing competent cell buffers and preparing competent cells, can solve the problems of low transformation efficiency of competent cells, achieve efficient and rapid preparation, increase permeability, and transformation efficiency Improved effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0034] The embodiment of the present invention provides a buffer solution for preparing competent cells, the buffer solution includes piperazine-1,4-diethanesulfonic acid, N,N-(2-hydroxyethyl)-2-amino An aqueous solution of ethanesulfonic acid, calcium chloride, potassium chloride and manganese chloride, the pH of the buffer is 6.7, in this example, 4.5354 g of piperazine-1,4-diethylsulfonate is added to the buffer Acid, 2.1325g N,N-(2-hydroxyethyl)-2-aminoethanesulfonic acid, 1.6647g calcium chloride, 14.9102g potassium chloride and 5.6656g manganese chloride, add water 1000ml, make piperazine-1, The concentrations of 4-diethanesulfonic acid, N,N-(2-hydroxyethyl)-2-aminoethanesulfonic acid, calcium chloride, potassium chloride and manganese chloride were 15mM, 10mM, 15mM, 200mM and 35mM, respectively , when preparing the buffer solution, adjust the pH value of the buffer solution with potassium hydroxide, and filter and sterilize, store the prepared buffer solution at 4°C for...
Embodiment 2
[0058] The embodiment of the present invention provides a buffer solution for preparing competent cells, the buffer solution includes piperazine-1,4-diethanesulfonic acid, N,N-(2-hydroxyethyl)-2-amino Aqueous solution of ethanesulfonic acid, calcium chloride, potassium chloride and manganese chloride, the pH of the buffer is 6.0, in this example, 3.326 g of piperazine-1,4-diethylsulfonate was added to 1 L of buffer Acid, 0.6398g N,N-(2-hydroxyethyl)-2-aminoethanesulfonic acid, 0.5549g calcium chloride, 3.7276g potassium chloride and 1.6187g manganese chloride, add water 1000ml, make piperazine-1, The concentrations of 4-diethanesulfonic acid, N,N-(2-hydroxyethyl)-2-aminoethanesulfonic acid, calcium chloride, potassium chloride and manganese chloride were 11 mM, 3 mM, 5 mM, 50 mM and 10 mM, respectively . Store the prepared buffer at 4°C until use.
[0059] The recipient bacterium selected in this embodiment is Escherichia coli, specifically Escherichia coli DH5α.
[0060] S...
Embodiment 3
[0069] The embodiment of the present invention provides a buffer solution for preparing competent cells, the buffer solution includes piperazine-1,4-diethanesulfonic acid, N,N-(2-hydroxyethyl)-2-amino Aqueous solution of ethanesulfonic acid, calcium chloride, potassium chloride and manganese chloride, the pH of the buffer is 7.0, in this example, 9.0708 g of piperazine-1,4-diethanesulfonic acid was added to the buffer , 4.265g N,N-(2-hydroxyethyl)-2-aminoethanesulfonic acid, 2.7745g calcium chloride, 22.3653g potassium chloride and 8.094g manganese chloride, add water 1000ml, make piperazine-1,4 - The concentrations of diethanesulfonic acid, N,N-(2-hydroxyethyl)-2-aminoethanesulfonic acid, calcium chloride, potassium chloride and manganese chloride are 30mM, 20mM, 25mM, 300mM and 50mM, respectively, When preparing the buffer solution, adjust the pH value of the buffer solution with potassium hydroxide, filter and sterilize, and store the prepared buffer solution at 4° C. until...
PUM
Property | Measurement | Unit |
---|---|---|
Conversion efficiency | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information

- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com