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A test strip for quantitatively detecting compounds and a detection method for lateral flow immunochromatography

A quantitative detection and detection method technology, applied in the field of medical detection, can solve the problems of not reaching the maximum accuracy and large intra-batch differences, and achieve the effect of improving detection accuracy

Active Publication Date: 2019-03-01
SUZHOU HAIMIAO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the detection method disclosed in the Chinese patent application No. CN201310391061.0 is suitable for traditional immunoassays, but it is found in the actual detection process that the biggest problem with this detection method is the intra-batch quality of the product. The difference is relatively large, basically around 15%, the best around 10%, and the detection accuracy has not reached the maximum

Method used

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  • A test strip for quantitatively detecting compounds and a detection method for lateral flow immunochromatography
  • A test strip for quantitatively detecting compounds and a detection method for lateral flow immunochromatography
  • A test strip for quantitatively detecting compounds and a detection method for lateral flow immunochromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Detection by the principle of double antibody (or double antigen) sandwich method:

[0017] Prepare 500 μl of different concentrations of CRP-BSA solutions, and conduct tests with different concentrations of the substances to be detected. See the table below for specific data.

[0018] The anti-CRP monoclonal antibody coupled with Eu microspheres and the rabbit antibody IgG coupled with Eu microspheres are evenly coated on the marker pad 4 of the test strip. In this embodiment, the molecular weight of the macromolecule is generally greater than 10,000, or about 10,000.

[0019] At the detection zone 5 of the NC membrane, dilute the anti-CRP monoclonal antibody to a concentration of 0.5-3mg / ml with coating diluent, and at the same time apply the goat anti-mouse antibody at a concentration of 0.5-2mg / ml to the first layer of the NC membrane. a control area.

[0020] At the same time, apply 0.2-1 mg / ml goat anti-rabbit antibody on the second control area of ​​...

Embodiment 2

[0033] Embodiment 2: Detection by the principle of competition law:

[0034] Specific test steps:

[0035]The molecular weight of the small molecule to be detected in this embodiment is less than 10,000. Dilute the small molecule antigen ketamine coupled with BSA to 0.5-3mg / ml with coating diluent, draw a film on the line detection area 5 of the NC membrane, and apply 0.5-2mg / ml goat anti-mouse antibody on the NC membrane The first control area 6 was drawn, and the 0.5-1 mg / ml goat anti-rabbit antibody was drawn on the second control area 7 of the NC membrane.

[0036] The anti-ketamine monoclonal antibody coupled to Eu microspheres and the rabbit antibody IgG coupled to Eu microspheres were evenly coated on the marker pad.

[0037] As shown in the table below, when samples containing different concentrations of ketamine to be detected are added to the sample pad, after 5-15 minutes of chromatography, the test strip is put into a special fluorescence reader to detect the res...

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Abstract

The invention discloses a test strip for quantitatively detecting a compound. The test strip comprises an NC membrane, a sample pad located at the front end of the NC membrane, a marker pad located on the inner side of the sample pad, a detection zone located behind the marker pad and a first control area located behind the detection zone, wherein a second control zone is further arranged behind the first control zone, the marker pad contains a monoclonal antibody, coupled with Eu microsphere, of a to-be-detected compound and a first antibody coupled with Eu microspheres, the detection zone contains a second antibody or antigen of the to-be-detected compound, the first control zone contains a third antibody, the second control zone contains a fourth antibody which does not react with the detected compound, the second antibody and the third antibody, and the fourth antibody only reacts with the first antibody. Compared with the prior art, the detection precision of the detected compound can be improved.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a test strip for quantitatively detecting compounds and a detection method for lateral immunochromatography. Background technique [0002] The existing method of lateral immunofluorescence chromatography to detect antigens or antibodies has two basic principles: one is the double-antibody (or double-antigen) sandwich method, and the monoclonal antibody against the antigen (macromolecule) to be tested is marked on the T At line C, another monoclonal antibody coupled to Eu microspheres was placed on the marker pad, and line C was marked with goat anti-mouse antibody. When the sample containing the antigen to be tested is added to the sample pad, the antigen to be tested is combined with the labeled antibody in the labeled pad, and the T line is chromatographically formed with the antibody here to form an antibody-antigen-labeled antibody complex, which stays at the T line. If the ...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558G01N21/64
CPCG01N21/6486G01N33/558G01N33/577
Inventor 谭淼
Owner SUZHOU HAIMIAO BIOTECH CO LTD