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Production method of silkworm regenerated silk fibroin with controllable molecular weight

A technology for regenerating silk fibroin and a production method, which can be applied to the preparation methods of peptides, chemical instruments and methods, peptide sources, etc., and can solve the problems of long time, energy consumption and low efficiency.

Active Publication Date: 2021-05-25
江苏伯夫保医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are costly, have sewage discharge, low efficiency, time-consuming, energy-consuming, and are non-standardized operations

Method used

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  • Production method of silkworm regenerated silk fibroin with controllable molecular weight
  • Production method of silkworm regenerated silk fibroin with controllable molecular weight
  • Production method of silkworm regenerated silk fibroin with controllable molecular weight

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Experimental program
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Effect test

Embodiment 1

[0049] The present invention proposes a production method of silkworm regenerated silk fibroin with controllable molecular weight, realizes zero addition in the degumming process, is green and environmentally friendly, energy-saving and high-efficiency, realizes the standardized preparation of regenerated silk fibroin, and controls the regenerated silk fibroin through parameter adjustment molecular weight and distribution. It is especially suitable for mass production of regenerated silk fibroin and strict requirements on the molecular weight and distribution of regenerated silk fibroin.

[0050] Such as figure 1 with figure 2 As shown, a method for producing silkworm regenerated silk fibroin with controllable molecular weight, the specific steps are:

[0051] Step 1: Degumming: Cut the cocoon silk into strips with a width of 1cm, put the cocoon silk and distilled water into the automatic degumming equipment for large-scale production of silk fibroin fiber at a ratio of 1g:...

Embodiment 2

[0059] The present invention proposes a production method of silkworm regenerated silk fibroin with controllable molecular weight, realizes zero addition in the degumming process, is green and environmentally friendly, energy-saving and high-efficiency, realizes the standardized preparation of regenerated silk fibroin, and controls the regenerated silk fibroin through parameter adjustment molecular weight and distribution. It is especially suitable for mass production of regenerated silk fibroin and strict requirements on the molecular weight and distribution of regenerated silk fibroin.

[0060] Such as figure 1 with figure 2 As shown, a method for producing silkworm regenerated silk fibroin with controllable molecular weight, the specific steps are:

[0061] Step 1: Degumming. The factory silk is put into the automatic degumming equipment for the large-scale production of silk fibroin fiber according to the ratio of 1g:100mL distilled water. The degumming temperature is ...

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Abstract

The invention provides a green, rapid and standardized production method of regenerated silk fibroin from silkworms with controllable molecular weight, which is characterized in that it includes: degumming; dissolving: degumming and drying the silk fibroin fiber according to the ratio of 1g:5-15mL solvent. Stir and dissolve, the dissolution temperature is 25-85°C, and the dissolution time is 30-60 minutes; dialysis: filter the dissolved silk fibroin solution with a 150-250 mesh filter, and then fill it into a dialysis bag. The volume of the solution accounts for the volume of the dialysis bag. 1 / 2-1 / 4, place the dialysis bag into the regenerated silk fibroin dialysis device, first dialyze with tap water for 6-18h at room temperature, and then dialyze with distilled water for 6-18h; drying: the dialyzed silk fibroin solution is freeze-dried , packaging and sterilization. The production method of the present invention can effectively control the molecular weight and distribution of regenerated silk fibroin from silkworms.

Description

technical field [0001] The invention relates to a method for producing silkworm regenerated silk fibroin with controllable molecular weight, and belongs to the technical field of biomedical material preparation. Background technique [0002] Bombyx mori silk fibroin molecules are secreted by the rear silk glands, coated with sericin secreted by the middle silk glands, and then passed through the front silk glands and spinning tubes to form silk fibers, also known as cocoons. Cocoon silk is processed into mill silk, the most common form of natural silk fiber. The factory silk can be woven, woven or knitted to form yarn or silk fabric, and then can be used for human consumption or medical use after degumming. Silk braided sutures have been used clinically for hundreds of years, and the research on silk fibroin fiber artificial blood vessels, artificial tendons and artificial ligaments has also achieved preliminary results. Regenerated silk fibroin is silk fibroin obtained af...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C07K1/36C07K1/34
CPCC07K14/43586
Inventor 关国平王璐王富军林婧邹婷
Owner 江苏伯夫保医疗科技有限公司