A method for establishing a rhesus monkey chronic kidney disease model with glomerulus and renal tubule compound damage
A technology for chronic kidney disease and rhesus monkey, which is applied in the field of establishing an animal model of rhesus monkey chronic kidney disease, can solve the problems of poor safety, few models of chronic kidney disease, and few models of renal tubular damage, etc., and achieves the effects of excellent application prospect and safe preparation method.
Active Publication Date: 2020-06-19
樊均明 +1
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Problems solved by technology
[0003] At present, the experimental subjects of applied basic research on chronic kidney disease are mainly rodents, and the application of the research results has great limitations. Non-human primates are currently the highest population in the animal kingdom. It is highly similar to humans, about 98.5% of its genes are homologous to humans, and it is the highest-level non-human chronic kidney disease model species. However, non-human primates are used to establish a chronic kidney disease model that simulates the occurrence and development of human chronic kidney disease. There are not many models of kidney disease, especially considering the glomerular and tubular injury models. At present, the literature only reports the induction of simian immunodeficiency virus SHIV. Because SHIV has the risk of human infection and poor safety, it is necessary to explore new and safe methods. The method to establish such a simulated human chronic kidney disease model lays a good model foundation for later research
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Embodiment 1
[0024] The preparation of embodiment 1 animal model of the present invention
[0025] 1 Materials and methods
[0026] 1.1 Main materials and reagents
[0027] Twelve female macaques were purchased from Sichuan Yibin Hengshui Biotechnology Co., Ltd. The initial body weight was 4.5±0.5kg, and the macaques were fed in a single cage and fed with special feed, twice a day, with an apple added after each feed, and clean drinking water.
[0028] PASM kit (article number 36-47, Beijing Huanyu Golden Eagle Technology), Masson kit (article number D026, Nanjing Jiancheng), adriamycin freeze-dried powder (product code: 1599494734, Shandong Pude Pharmaceutical), cisplatin (product No.: A14200156601, Qilu Pharmaceutical), and the rest are pure reagents for routine chemical analysis.
[0029] 1.2 Modeling and grouping
[0030] Twelve female rhesus monkeys, body weight (4.5±0.5kg), were divided into 4 groups, 3 in each group. They were normal group, doxorubicin group, cisplatin group an...
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The invention discloses a combined use of adriamycin and / or cis-platinum, and relates to the combined use of the adriamycin and the cis-platinum in preparation of an animal model for screening and treating chronic nephrosis, wherein the application dosage of the adriamycin is 1-2mg / Kg / time, the application dosage of the cis-platinum is 2mg / Kg / time, and an animal used for the model is a primate animal. The invention also discloses a method for preparing the animal model for the chronic nephrosis. After the method is adopted, the animal model for macaque chronic nephrosis with glomerulus and renal tubule interstitium compound injury can be effectively established; furthermore, the model is safe in preparation method, can be used for screening related drugs and has good application prospect.
Description
technical field [0001] The present invention relates to the establishment of an animal model of chronic kidney disease, specifically, the establishment of an animal model of rhesus monkey chronic kidney disease accompanied by glomerular and tubulointerstitial compound damage. Background technique [0002] Chronic kidney disease (CKD) caused by various pathological factors is one of the main risk factors for cardiovascular disease, premature aging and end-stage renal disease in humans. The incidence of chronic kidney disease in adults is 8%-12%. In western countries, the prevalence of chronic kidney disease reaches 25% among the elderly over 65 years old; in China, it is about 11.5%, and the urban (13%) is higher than the rural (10%). Among CKD patients, only 8.7% were diagnosed and 4.9% were treated. CKD has the characteristics of high incidence, high prevalence of concomitant cardiovascular disease, and high mortality rate; low awareness rate of CKD, low prevention rate, ...
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IPC IPC(8): A61K31/704A61K33/243A61K49/00
CPCA61K31/704A61K33/24A61K49/0008
Inventor 樊均明陈波王丽王正武谭睿陟
Owner 樊均明