Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Artificial polypeptides and their biological products for inducing differentiation of bone marrow mesenchymal stem cells into hepatocytes

A bone marrow mesenchymal and stem cell technology, applied in the field of stem cells, can solve the problems of not being able to maintain the original characteristics, lack of cell sources, etc.

Active Publication Date: 2020-05-15
诺赛联合(北京)生物医学科技有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Liver cell transplantation is one of the most effective methods for the treatment of end-stage liver failure, but its clinical application is affected by factors such as lack of cell sources, difficulty in mass proliferation in vitro, failure to maintain its original characteristics after passage, and immune rejection.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Artificial polypeptides and their biological products for inducing differentiation of bone marrow mesenchymal stem cells into hepatocytes
  • Artificial polypeptides and their biological products for inducing differentiation of bone marrow mesenchymal stem cells into hepatocytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] The substantive content of the present invention will be specifically introduced below in conjunction with the embodiments. Due to space reasons, the description of the experimental process cannot be done in great detail. All the parts not described in detail in the experiment are routine operations well known to those skilled in the art.

[0027] 1. Experimental materials

[0028] The polypeptide involved in the present invention is synthesized by the standard polypeptide solid-phase synthesis technique well known in the art, using the N-terminal protection strategy of fluorenylmethoxycarbonyl (Fmoc). According to the method of resin solid-phase synthesis, the corresponding amino acids are sequentially connected, during which the Fmoc-protecting group is sequentially removed, and then the peptide is cut to obtain a crude product. The crude product is separated and purified by a C18 column to obtain the polypeptide represented by formula (IV).

[0029] HPLC and MS detec...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an artificial polypeptide capable of inducing bone mesenchymal stem cells to differentiate into hepatic cells and a biological product of such artificial polypeptide. The artificial polypeptide is a polypeptide containing an amino acid sequence shown in formula (IV): LGENQPDAK(Xa)PCFQEDPMA(Xb)GTDCTLMEIWN, (IV), wherein each of Xa and Xb is selected from M, Y, L, V, W or E. Professionals in the field know that a strong ability to induce the bone mesenchymal stem cells to differentiate into the hepatic cells is achieved in case of combination of acidic fibroblast growth factor, hepatocyte growth factor and oncostatin M, and after induced culture for 14 days, alpha fetal protein mRNA and albumin mRNA in the cells are in remarkably positive expression. It is proven that the artificial polypeptide with the same effect is capable of inducing the bone mesenchymal stem cells to differentiate into the hepatic cells, and accordingly the artificial polypeptide can be prepared into the biological product for inducing the bone mesenchymal stem cells to differentiate into the hepatic cells.

Description

technical field [0001] The invention belongs to the field of stem cells, and relates to directional induced differentiation of stem cells, in particular to an artificial polypeptide for inducing bone marrow mesenchymal stem cells to differentiate into liver cells and biological products thereof. Background technique [0002] Hepatocyte transplantation is one of the most effective methods for the treatment of end-stage liver failure, but its clinical application is affected by factors such as lack of cell sources, difficulty in mass proliferation in vitro, failure to maintain its original characteristics after passage, and immune rejection. With the progress of tissue engineering research, stem cell therapy for end-stage liver disease has become the most promising development direction. [0003] Bone marrow mesenchymal stem cells are a kind of non-hematopoietic stem cells first reported and discovered by Friedenstein et al. in the early 1970s. Because these cells are spindle...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00C12N5/071
CPCC07K14/00C12N5/067C12N2501/998C12N2506/1353
Inventor 王莉李岩孙天娇徐梦龙胡芳
Owner 诺赛联合(北京)生物医学科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com