NPC (nasopharyngeal carcinoma) related tumor marker and application

A tumor marker, nasopharyngeal cancer technology, applied in the field of tumor molecular biology, can solve the problem of unclear lncRNA function, and achieve important promotion and application prospects and profound clinical significance.

Active Publication Date: 2017-08-18
CHENZHOU NO 1 PEOPLES HOSPITAL
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Problems solved by technology

Thousands of lncRNAs have been reported to exist in the human ge

Method used

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  • NPC (nasopharyngeal carcinoma) related tumor marker and application
  • NPC (nasopharyngeal carcinoma) related tumor marker and application
  • NPC (nasopharyngeal carcinoma) related tumor marker and application

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Experimental program
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Embodiment Construction

[0017] The kit for auxiliary diagnosis, curative effect prediction and prognosis judgment of nasopharyngeal carcinoma includes:

[0018] (1) Reagents for extracting total RNA from nasopharyngeal carcinoma and cancer disc tissues, including Trizol reagent, chloroform, isopropanol, and DEPC water.

[0019] (2) Reagents used for reverse transcription of total RNA into cDNA: promega reverse transcription kit (GoScript TM Reverse Transcriptase, A5003), including: GoScript TM 5X Reaction Buffer, MgCl 2 , PCR NucleotideMix, Recombinant Ribonuclease Inhibitor, GoScript TM Reverse Transcriptase, Nuclease-Free Water.

[0020] (3) Reagents used for cDNA real-time fluorescent quantitative PCR, including lncRNA-KAT7 real-time fluorescent quantitative PCR specific primers, GAPDH internal reference specific primers, real-time fluorescent quantitative SYBR dye (Roche 4887352001 LightCycler480 SYBR Green I Master), DEPC water.

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Abstract

The invention discloses an NPC (nasopharyngeal carcinoma) related tumor marker and an application. The tumor marker is lncRNA, is positioned at the 17th chromosome chr17:47785696-47797422 and has the nucleotide sequence shown in SEQ ID NO.1 and named as lncRNA-KAT7. According to the sequence lncRNA-KAT7, the specific real-time fluorescent quantitative PCR primer is designed and synthesized, and a preparation used for NPC diagnosis or curative effect prediction is prepared. Detection of the expression level of lncRNA-KAT7 in an NPC clinical case specimen by use of the real-time fluorescent quantitative PCR preparation proves that expression of lncRNA-KAT7 in NPC is remarkably reduced, and lncRNA-KAT7 can be used for preparing a preparation or a kit for auxiliary diagnosis, curative effect prediction and prognosis of NPC.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and relates to a tumor marker related to nasopharyngeal carcinoma and its application. Background technique [0002] Although nasopharyngeal carcinoma (NPC) is rare, it has a high incidence in Southeast Asia. About 80% of nasopharyngeal carcinoma occurs in China, so nasopharyngeal carcinoma is also known as "Chinese cancer". The latest report predicts that there will be 60,000 new patients with nasopharyngeal carcinoma in China in 2015. The incidence of nasopharyngeal cancer in the Huguang area where Hunan is located is as high as 20 / 100,000 to 30 / 100,000, which is the hardest hit area for nasopharyngeal cancer. Recently, some scholars suggested that early screening of nasopharyngeal carcinoma should be included in the routine physical examination in Hunan. Nasopharyngeal carcinoma is related to multiple factors such as Epstein-Barr virus (EBV), environmental factors and genetics. The o...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/166C12Q2600/178
Inventor 罗迪贤贺荣章王晴美胡政罗伟濠李佳段丽丽罗文娜
Owner CHENZHOU NO 1 PEOPLES HOSPITAL
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