Primers and kits for double PCR detection of duck adenovirus type 2 and duck adenovirus type A

Abstract

The invention provides a dual PCR detection probe and kit for duck adenovirus 2 and duck adenovirus A, and belongs to the field of epizootiology. Two pairs of primers which are shown in SEQ ID NO.1-4 are adopted, a dual PCR detection method through which differential diagnosis can be conducted on duck adenovirus 2 and duck adenovirus A in a duck flock is built, a basis is laid for developing an epidemiological survey of adenovirus infection types and scientific prevention and control of relevant diseases in the duck flock, and thus the dual PCR detection probe and kit for duck adenovirus 2 and duck adenovirus A have very important study significance.

Description

technical field [0001] The invention provides double PCR detection primers and kits for duck adenovirus type 2 and duck adenovirus type A, belonging to the field of animal infectious diseases. Background technique [0002] According to the classification of the International Committee on Taxonomy of Viruses, Adenoviridae consists of five genera, namely, AT-rich adenovirus, avian adenovirus, fish adenovirus, mammalian adenovirus and sialidase virus. The total length of the adenovirus genome is about 33 kd, and the genome is linear double-stranded DNA; the virus-like particles are non-enveloped nucleocapsids with icosahedral symmetry, and the nucleocapsids of adenoviruses have three main structural proteins, respectively It is Hexon, Fiber and Penton. Among them, the Hexon protein is the most important structural protein of viruses belonging to the family Adenoviridae, which contains the main protective antigen gene cluster of the virus and is closely related to the pathogeni...

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Problems solved by technology

[0004] Currently, there are 2 pathogenic species of Adenoviridae but belonging to different genera of Adenoviridae in duck populations: duck adenovirus type 2 (belonging to the genus Avian Adenovirus in the family Adenoviridae) and duck adenovirus type A (belonging to the AT-rich family of Adenoviridae). adenovirus), however, there are currently no research reports on primers that can simultaneously perform dual PCR detection methods for duck adenovirus type 2 and duck adenovirus type A at home and abroad. The establishment of the present invention can fill in the gaps in related fields at home and abroad

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