Rapid propagation method of apple stock B9

Abstract

The invention belongs to the technical field of culturing of apple seedlings, and discloses a rapid propagation method of apple stock B9. The rapid propagation of the apple stocks can be realized by adding three-fifths field soil and upper part two-fifths vermiculite acclimating and transplanting through primary culture adopting the culture medium MS+6-BA0.6mg / L+IBA0.1mg / L+PVP600mg / L, subculture treated by MS+6-BA0.8mg / L+IBA0.1mg / L, and rooting culture adopting 1 / 2MS+IBA1.4mg / L+melatonin 0.01mg / L, not only can higher survival rate be guaranteed, but also the excellent property of the stocks can be kept, and convenient and fast method and approach can be provided for developing and further expanding apple industry; and the invention provides a rapid propagation method for culturing apple seedlings in China, so as to quicken the large-scale upgrading of apple in China.

Description

technical field [0001] The invention belongs to the technical field of apple seedling cultivation, in particular to a method for rapid propagation of apple rootstock B9. Background technique [0002] A cold-resistant dwarf rootstock bred by crossing M9 with RedStandard by Professor Budagovsky of Michurin University in the former Soviet Union. B9 was adopted as the main cold-resistant intermediate stock in the former Soviet Union and Poland, and it is also one of the rootstocks generally reflecting better drought resistance in the world. B9 is used as a dwarf stock, and after grafting, it is about 45% of the tree stock, with slightly large and small feet, and the middle stock heals well. At present, my country's apple production model has gradually changed from the traditional low-efficiency canopy orchard model to the modern low-stock dense planting intensive and high-efficiency production and cultivation model, and gradually transformed from the world's largest apple produ...

AI Technical Summary

Problems solved by technology

At present, the dwarf apple seedling market in my country is in short supply, and the key to restricting the development of the seedling industry is that, on the one hand, there is a lack of adaptable and excellent dwarf rootstocks, and on the other hand, no breakthrough has been made in the existing dwarf rootstock breeding technology.

[0003] In summary, the problems in the prior art are: at present, the dwarf apple seedlings in my country lack wide adaptability and excellent dwarf rootstocks, and no breakthrough has been made in the existing dwarf rootstock breeding technology.

[0004] In summary, the problems in the prior art are: (1) rootstock explant browning is more serious, affecting the survival rate

(2) Subculture hormone concentration ratio is not suitable, resulting in too high proportion of vitrified seedlings

(3) Improper ratio of substrate for seedling hardening and transplanting, resulting in poor air permeability and poor growth of seedlings due to lack of nutrients in the later stage

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