A gene-photothermal combined nanoparticle and its preparation method and application

Abstract

The present invention relates to preparation and antitumor applications of photothermal-gene combined treatment nanoparticles, and provides a preparation method of gene-loading nanoparticles based on OMCN and PEI, wherein the advantages of each component are effectively provided, and the obtained gene-loading nanoparticles are used for photothermal-gene combined treatment of breast cancer, and provide excellent treatment effects compared to the single treatment. According to the present invention, compared to the pure OMCN, the prepared OMCN grafted PEI vector has the following characteristics that the absorption at 808 nm after surface modification on PEI is increased, and the heat production capacity increased; compared to the pure PEI, the prepared OMCN grafted PEI vector has the following characteristics that the cytotoxicity is decreased and the gene transfection ability is enhanced by using OMCN as the support; and the new preparation technology for the efficient and low-toxicity photothermal-gene combined treatment nanoparticles is provided, wherein the efficient and low-toxicity photothermal-gene combined treatment nanoparticles completely provide the advantages of thermotherapy and photothermal therapy so as to effectively improve the tumor treatment effect.

Description

technical field [0001] The invention belongs to the field of biological technology and relates to gene-loaded nanoparticles, in particular to a gene-photothermal combined nanoparticle, its preparation method and its application in the preparation of anti-tumor preparations. Background technique [0002] According to the data, in the intervention of tumor treatment, the design and preparation of a multifunctional treatment platform can effectively integrate two treatment modes such as surgery, chemotherapy, radiotherapy, gene therapy, photothermal therapy or photodynamic therapy, etc., can reduce the Drug dosage, reducing side effects, overcoming multi-drug resistance, and improving anti-tumor effects have attracted widespread attention from scholars at home and abroad; such intelligent nano-platforms usually need to fully combine the advantages of organic and inorganic hybrid nanomaterials to achieve better results. play a combined therapeutic effect. [0003] Among them, f...

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Problems solved by technology

[0003] Among them, first of all, gene therapy is a potential tumor treatment method; because exogenous genes are easily degraded in body fluids and the efficiency of cell transfection is low, gene therapy needs to use vectors to achieve effective gene transfection; in many current Among gene carriers, viral vectors have high transfection efficiency, but there are potential safety hazards such as immunogenicity; non-viral vectors are relatively safe, but their transfection efficiency is limited; polyethyleneimine (PEI for short) which has been researched in recent years is not Viral gene vectors, because of their easy endosome escape and high transfection efficiency, have become the gold standard of gene therapy vectors; however, excessive use of PEI, especially high molecular weight PEI, will cause severe cytotoxicity and hemolysis; to overcome this Defects, there are studies on grafting PEI onto inorganic nanoparticles, such as the surface of mesoporous nanospheres with a large specific surface area, using it as a support. On the one hand, it can significantly reduce the amount of PEI used on the basis of ensuring effective gene transfection; on the other hand On the one hand, the special three-dimensional spherical structure of mesoporous nanospheres has low surface energy and small steric hindrance, which is conducive to further surface modification, and its internal pore structure can be used to load small molecule drugs to achieve multimodal combined therapy; therefore, PEI-grafted mesoporous nanomaterials have significant advantages in tumor gene therapy;

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