A kind of induced mulberry leaves for preventing and treating silkworm bmnpv disease and its application

A technology of mulberry leaves and silkworm, applied in the field of insect breeding, can solve the problem of no clear method for the treatment of BmNPV disease, and achieve the effect of reducing the mortality rate of silkworm

Active Publication Date: 2020-08-28
开化明阳健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no clear way to treat BmNPV disease

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Freshly picked mulberry leaves in June were irradiated by UVB ultraviolet light with an intensity of 1000W for 10 minutes, and the air humidity was kept at 40% during the irradiation process. The mulberry leaves obtained in this way were induced mulberry leaves. The silkworms were divided into two groups. One group was raised with normal fresh mulberry leaves, and the other group was raised with induced mulberry leaves. Each group had 90 silkworms. They were inoculated with 200 μL BmNPV on the first day of the second instar, and 5 different concentrations of viruses were sprayed on the mulberry leaves. , namely: 1.22×10 4 units / mL, 1.22×10 5 units / mL, 1.22×10 6 units / mL, 1.22×10 7 units / mL, 1.22×10 8 units / mL. In this experiment, water was used instead of virus as a control group. It was found that the median lethal concentration (LC50) of BmNPV in the silkworm group fed normal fresh mulberry leaves was (1.86±0.15)×10 6 unit / mL, the LC50 of BmNPV in the silkworm g...

Embodiment 2

[0018] Freshly picked mulberry leaves in July were irradiated with 200W UVA ultraviolet light for 30 minutes, and the air humidity was kept at 50% during the irradiation process. The mulberry leaves obtained in this way were induced mulberry leaves. The silkworms were divided into two groups. One group was raised with normal fresh mulberry leaves, and the other group was raised with induced mulberry leaves. Each group had 90 silkworms. They were inoculated with 200 μL BmNPV on the first day of the second instar, and 5 different concentrations of viruses were sprayed on the mulberry leaves. , namely: 1.22×10 4 units / mL, 1.22×10 5 units / mL, 1.22×10 6 units / mL, 1.22×10 7 units / mL, 1.22×10 8 units / mL. In this experiment, water was used instead of virus as a control group. It was found that the LC50 of BmNPV in the silkworm group fed normal fresh mulberry leaves was (1.83±0.09)×10 6 unit / mL, the LC50 of BmNPV in the silkworm group fed with induced mulberry leaves was (2.51±0....

Embodiment 3

[0020] Freshly picked mulberry leaves in August were irradiated by UVB ultraviolet light with an intensity of 20W for 2 hours, and the air humidity was kept at 60% during the irradiation process. The mulberry leaves obtained in this way were induced mulberry leaves. The silkworms were divided into two groups. One group was raised with normal fresh mulberry leaves, and the other group was raised with induced mulberry leaves, with 90 silkworms in each group. They were inoculated with 200 μL BmNPV on the first day of the third instar, and 5 different concentrations of viruses were sprayed on the mulberry leaves. , namely: 1.22×10 4 units / mL, 1.22×10 5 units / mL, 1.22×10 6 units / mL, 1.22×10 7 units / mL, 1.22×10 8 units / mL. In this experiment, water was used instead of virus as a control group. It was found that the median lethal concentration (LC50) of BmNPV in the silkworm group fed normal fresh mulberry leaves was (1.94±0.35)×10 6 unit / mL, the LC50 of BmNPV in the silkworm g...

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Abstract

The invention discloses induced mulberry leaves for preventing bombyx mori from suffering from BmNPV diseases and an application of the induced mulberry leaves. Bombyx mori nuclear polyhedrosis viruses (BmNPV) are one of viruses causing largest damage to bombyx mori, and at present, an explicit treatment method for the BmNPV diseases is not developed. According to the induced mulberry leaves disclosed by the invention, fresh mulberry leaves are subjected to ultraviolet illumination, wherein ultraviolet rays are one or more of UVA wave bands, UVB wave bands and UVC wave bands, the ultraviolet illumination intensity is 10-1000W, the ultraviolet illumination time is 0.05-3h, and the humidity in the illumination process is kept to be 10-100%. The mulberry leaves have important pharmacological activity and a main food source for the bombyx mori. According to the induced mulberry leaves, the content of mulberry leaf secondary metabolism products can be notably changed, the mortality rate of the bombyx mori after being infected with viruses is reduced, and the bombyx mori nuclear polyhedrosis viruses can be resisted.

Description

technical field [0001] The invention belongs to insect breeding, and in particular relates to an induced mulberry leaf for preventing and treating BmNPV disease of silkworm and its application. Background technique [0002] Silkworm (Bombyx mori), Lepidoptera, Bombyridae, spins cocoons into silk and feeds on mulberry leaves. Bombyx mori nuclear polyhedrosis virus (BmNPV) particles are rod-shaped, belonging to the family Baculoviridae, the genus Baculovirus, the species of Bombyx mori nuclear polyhedrosis virus, and is a representative species of mononuclear capsid nucleopolyhedrosis virus. Its outer capsule, containing double-stranded DNA molecules, is one of the most sensitive and common pathogens of silkworm species. After infection, silkworms suppurate and die. The reaction is violent and rapid, which in turn causes huge economic losses in the sericulture industry. After the virus infects the silkworm, ODV is released in the alkaline midgut fluid. ODV passes through the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/30A23K50/90A61K36/605A61P31/20
CPCA23K10/30A23K50/90A61K36/605
Inventor 朱玮田景奎杨丙贤张琳胡瑾虞姣姣管祺杰
Owner 开化明阳健康科技有限公司
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