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A preparation method and application of phosphorus-accumulating bacteria imprinted biochar

A technology of biochar and phosphorus-accumulating bacteria, applied in the direction of using microorganisms, chemical instruments and methods, biological water/sewage treatment, etc., can solve the limitations of adsorption performance and stability, limited adsorption sites, and reduce the mobility of heavy metals in the environment , bioavailability and other issues

Active Publication Date: 2021-02-02
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biochar prepared by pyrolysis and modification has a certain effect of adsorbing heavy metals, which can reduce the mobility and bioavailability of environmental heavy metals, and has been widely studied. However, due to the limited adsorption sites on the surface of a single biochar, its Adsorption performance and stability are greatly limited

Method used

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  • A preparation method and application of phosphorus-accumulating bacteria imprinted biochar
  • A preparation method and application of phosphorus-accumulating bacteria imprinted biochar
  • A preparation method and application of phosphorus-accumulating bacteria imprinted biochar

Examples

Experimental program
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Effect test

Embodiment 1

[0019](1) Take 5~10g of sawdust and conduct anaerobic constant temperature pyrolysis at 200-700℃ for 4h; after complete carbonization, cool to room temperature and take it out to obtain biochar. Mix and grind the biochar, pass through a 100 mesh sieve, and place it in a desiccator Save in

[0020](2) Take 10g of activated sludge into 90ml of sterile water and mix well, let it stand at room temperature for 10min, take the supernatant and make it into 6 kinds of bacteria suspension with the concentration of 10-1 to 10-6 by 10-fold dilution method. , Respectively take 0.2ml of the bacterial solution of different concentrations and evenly spread it on the solid phosphorus-rich plate, incubate at 30℃ for 24h, in the gradient plate with obvious single colony, pick the single colony and transfer it to the phosphorus-rich medium Repeatedly purify the above until the single colony on the plate has the same morphology to obtain the primary pure strain, which is connected to the LB solid medium s...

Embodiment 2

[0037]The application of the phosphorus-accumulating bacteria imprinted biochar of the present invention to the treatment of Cr ions in wastewater includes the following steps: taking 100 mL of a Cr6+ solution with an initial concentration of 10-100 mg / L, adjusting the pH of the solution to 6.0, and adding Example 1 The prepared phosphorous accumulating bacteria imprinted biochar adsorbent, the amount of the adsorbent is 0.25g / L, the adsorption reaction is carried out at a constant temperature oscillator of 30℃ for 3 hours, and then the adsorbent is separated from the wastewater, and the wastewater is measured by AAS The content of the remaining Cr6+ in, the calculated adsorption results are shown in Table 1:

[0038]Table 1: The adsorption capacity of phosphorus-accumulating bacteria imprinted biochar on wastewater with different initial concentrations of Cr6+

[0039]

[0040]It can be seen from Table 1 that under the condition of the initial concentration of 10.01mg / L, the adsorbent has a...

Embodiment 3

[0042]The phosphorus-accumulating bacteria imprinted biochar of the present invention is used to treat radioactive metal UO2+ ions in wastewater, including the following steps: take 100 mL of UO2+ solution with an initial concentration of 10-100 mg / L, adjust the pH of the solution to 6.0, and add it for implementation Phosphorus-accumulating bacteria imprinted biochar adsorbent prepared in Example 1, the amount of the adsorbent is 0.25g / L, the adsorption reaction is carried out at a constant temperature oscillator of 30°C for 3 hours, and then the adsorbent is separated from the wastewater and used AAS The content of UO2+ remaining in the waste water was determined, and the calculated adsorption results are shown in Table 1:

[0043]Table 2: Phosphorus-accumulating bacteria imprinted biochar for different Cr6+Adsorption capacity of initial concentration wastewater

[0044]

[0045]It can be seen from Table 2 that the adsorbent has an adsorption capacity of 17.13mg / g under the condition of an...

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Abstract

A preparation method and application of phosphorus-accumulating bacteria imprinted biochar according to the present invention. The biochar loaded with phosphorus-accumulating bacteria provided by the present invention can provide a good living environment for microorganisms, and can also help phosphorus-accumulating bacteria to improve resistance to high salinity And the ability of heavy metals, while the number of adsorption sites increases, and has considerable stability, the adsorption performance of heavy metals is greatly improved, the product is non-toxic, and environmentally friendly. The preparation method provided by the invention is simple and easy for industrial production.

Description

Technical field[0001]The invention relates to a preparation method of phosphorus accumulating bacteria imprinted biochar, in particular to a preparation method and application of phosphorus accumulating bacteria imprinted biochar.Background technique[0002]Every year in my country, a large amount of sawdust, straw and other biomass are burned and wasted, which will not only pollute the environment[0003]Hazards will also cause waste and loss of bio-energy. How to make full and reasonable use of such "waste" to turn waste into treasure is a problem that needs to be solved urgently. The biochar prepared by pyrolysis and modification has a certain effect of adsorbing heavy metals, which can reduce the mobility and bioavailability of heavy metals in the environment. It has been extensively studied. However, a single biochar has very limited adsorption sites on its surface. Adsorption performance and stability are greatly restricted. Therefore, research and development of a new type of ads...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N1/36C02F3/34C02F101/20
CPCC02F3/34C02F2003/003C02F2101/20C12N1/36C12N11/14
Inventor 谭文发丁蕾江雨萌范嘉庆吕俊文张杰谢超罗洋
Owner NANHUA UNIV
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